Beta adrenergic stimulation of endogenous epidermal cyclic AMP formation

Beta Adrenergic Receptors in Keratinocytes

“…The first studies to suggest the presence of betaAR in the epidermis were functional ones that demonstrated an increase in levels of cAMP in keratinocytes after stimulation with non-specific betaAR agonists [8,9]. Subsequently, work using agonists and antagonists with specificity for the different adrenergic receptor subtypes demonstrated that the increase in keratinocyte…”

“…Studies by Voorhees and colleagues and Flaxman and Harper over 30 years ago demonstrated the regulation of keratinocyte proliferation by intracellular levels of cAMP, and that elevation of intracellular cAMP by catecholamine stimulation resulted in a decrease in proliferation [8,9,[63][64][65]. Work by these laboratories also suggested that a decrease in the ability of psoriatic keratinocytes to respond to beta adrenergic agonists with an increase in cAMP could be, in part, responsible for the increase in cell proliferation characteristic of the disease [66][67][68][69].…”

“…The beta adrenergic receptor is a classic seven transmembrane G protein coupled receptor. These receptors serve as the endogenous receptor to the catecholamine hormones norepinephrine and epinephrine, and can be further subdivided into beta1, beta2, and beta3 subtypes, based on their differential pharmacological response to catecholamines and specific antagonists, as well as differences in their protein sequences [5][6][7].The first studies to suggest the presence of betaAR in the epidermis were functional ones that demonstrated an increase in levels of cAMP in keratinocytes after stimulation with non-specific betaAR agonists [8,9]. Subsequently, work using agonists and antagonists with specificity for the different adrenergic receptor subtypes demonstrated that the increase in keratinocyte…”

In vitro Noradrenaline Accumulation by Sheep Skin: Partial Characterization and Localization

“…The predominant site of [3H]NA uptake is cocaine-sensitive and, since active uptake is abolished in 60HDA-treated skin, may reasonably be considered to be sympathetic nerves. This localization of uptake sites does not allow correlation of the uptake with observed oc-adrenoceptor-mediated non-vascular effects of intradermally administered NA on wool growth (Cunningham et al 1979), or with the reported epidermal p-adrenoceptor-mediated antimitotic effect of NA (Powell et al 1971).…”

Biochemical studies of taste sensation: II. Labeling of cyclic amp of bovine taste papillae in response to Sweet and bitter stimuli

“…Those in papillae include, for instance, epithelial cells and nerve fibers (e.g., Beidler, 1969;Zalewski, 1968). Adenylate cyclase is widely distributed among tissues (Sutherland et al, 1962), including mammalian skin (Brdnstad et al, 1971;Powell et al, 1971;Mier and Urselmann, 1970). The presence of activity in the control tissues without taste buds (Table I) is therefore not surprising, but may be at variance with other results of Kurihara and Koyama (1972), who found a high level of adenylate cyclase in a particulate preparation derived from a homogenate of taste papillae, but very little activity in a similar preparation from nontaste tongue epithelium.…”