2013
DOI: 10.18632/aging.100593
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Abstract: Berberine (BRB), a natural alkaloid, has a long history of medicinal use in both Ayurvedic and old Chinese medicine. Recently, available as a dietary supplement, Berberine is reported to have application in treatment of variety diseases. Previously we observed that BRB inhibited mTOR/S6 signaling concurrently with reduction of the level of endogenous oxidants and constitutive DNA damage response. We currently tested whether Berberine can affect premature, stress-induced cellular senescence caused by mitoxantro… Show more

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Cited by 54 publications
(45 citation statements)
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References 89 publications
(161 reference statements)
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“…38,[46][47][48] In particular, hypoxia, starvation and contact inhibition all suppress geroconversion. 38,[47][48][49] As we will discuss, this can explain how quiescent cells remain quiescent in the organism for so long.…”
Section: P21-and P16 -Induced Senescencementioning
confidence: 98%
“…38,[46][47][48] In particular, hypoxia, starvation and contact inhibition all suppress geroconversion. 38,[47][48][49] As we will discuss, this can explain how quiescent cells remain quiescent in the organism for so long.…”
Section: P21-and P16 -Induced Senescencementioning
confidence: 98%
“…Such treatment causes persistent DNA replication stress. Berberine (BRB), a naturally occurring alkaloid with a long history of use in traditional Chinese and Ayurvedic medicine, is used as an example of a potential anti-aging agent (Lee et al, 2006; Halicka et al, 2012; Zhao et al, 2013). A549 cultures are treated with Mxt with or without BRB, and the depth of cellular senescence is assessed and compared between BRB-treated and untreated cultures.…”
Section: Quantitative Analysis Of Changes In Cell Morphology Associatmentioning
confidence: 99%
“…This methodology can also be used to measure senescence-associated changes in other cell types (e.g., WI-38), as well as changes in other biomarkers, such as induction of SA-β-gal or γH2AX and activation of ATM (Zhao et al, 2010, 2013; Halicka et al, 2012; McKenna et al, 2012; Zhao and Darzynkiewicz, 2013). Activation of SA-β-gal is measured using the Chemicon Cellular Senescence Assay Kit (Millipore) and the capability of LSC to measure both light absorption and intensity of fluorescence (Bedner et al, 1999; Darzynkiewicz et al, 1999; Henriksen et al, 2011; Zhao et al, 2013).…”
Section: Quantitative Analysis Of Changes In Cell Morphology Associatmentioning
confidence: 99%
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