Berberine suppresses gero-conversion from cell cycle arrest to senescence

Zhao, Hong; Halicka, H. Dorota; Li, Jiangwei; Darzynkiewicz, Zbigniew

doi:10.18632/aging.100593

Cited by 54 publications

(45 citation statements)

References 89 publications

(161 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…38,[46][47][48] In particular, hypoxia, starvation and contact inhibition all suppress geroconversion. 38,[47][48][49] As we will discuss, this can explain how quiescent cells remain quiescent in the organism for so long.…”

Section: P21-and P16 -Induced Senescencementioning

confidence: 98%

Geroconversion: irreversible step to cellular senescence

2014

View full text Add to dashboard Cite

Section: P21-and P16 -Induced Senescencementioning

confidence: 98%

Geroconversion: irreversible step to cellular senescence

2014

View full text Add to dashboard Cite

“…Such treatment causes persistent DNA replication stress. Berberine (BRB), a naturally occurring alkaloid with a long history of use in traditional Chinese and Ayurvedic medicine, is used as an example of a potential anti-aging agent (Lee et al, 2006; Halicka et al, 2012; Zhao et al, 2013). A549 cultures are treated with Mxt with or without BRB, and the depth of cellular senescence is assessed and compared between BRB-treated and untreated cultures.…”

Section: Quantitative Analysis Of Changes In Cell Morphology Associatmentioning

confidence: 99%

“…This methodology can also be used to measure senescence-associated changes in other cell types (e.g., WI-38), as well as changes in other biomarkers, such as induction of SA-β-gal or γH2AX and activation of ATM (Zhao et al, 2010, 2013; Halicka et al, 2012; McKenna et al, 2012; Zhao and Darzynkiewicz, 2013). Activation of SA-β-gal is measured using the Chemicon Cellular Senescence Assay Kit (Millipore) and the capability of LSC to measure both light absorption and intensity of fluorescence (Bedner et al, 1999; Darzynkiewicz et al, 1999; Henriksen et al, 2011; Zhao et al, 2013).…”

Section: Quantitative Analysis Of Changes In Cell Morphology Associatmentioning

confidence: 99%

“…Activation of SA-β-gal is measured using the Chemicon Cellular Senescence Assay Kit (Millipore) and the capability of LSC to measure both light absorption and intensity of fluorescence (Bedner et al, 1999; Darzynkiewicz et al, 1999; Henriksen et al, 2011; Zhao et al, 2013). …”

Section: Quantitative Analysis Of Changes In Cell Morphology Associatmentioning

confidence: 99%

“…In Ctrl cells, intensity of the mean value of maximal pixels is highest in mitotic (M) and immediately post-mitotic (pM) G 1 cells; nuclei of these cells also have a small area of DAPI fluorescence, consistent with their high degree of chromatin condensation (Bedner et al, 1999; Darzynkiewicz et al, 1999; Henriksen et al, 2011). In senescing cells, nuclear area increases while maximal pixel intensity decreases (Zhao et al, 2010, 2013; McKenna et al, 2012). These morphometric changes reflect enlargement of the projected nuclear area and decreased intensity of DAPI local staining per unit area due to a flattened cellular appearance, the hallmark of cellular senescence (Dimri et al, 1995; Itahana et al, 2007).…”

Section: Figure 9471mentioning

confidence: 99%

See 2 more Smart Citations

Attenuation of Replication Stress–Induced Premature Cellular Senescence to Assess Anti‐Aging Modalities

Zhao

Darzynkiewicz

2014

Current Protocols in Cytometry

Self Cite

View full text Add to dashboard Cite

Described is an in vitro model of premature senescence in pulmonary adenocarcinoma A549 cells induced by persistent DNA replication stress in response to treatment with the DNA damaging drug mitoxantrone (Mxt). The degree of cellular senescence, based on characteristic changes in cell morphology, is measured by laser scanning cytometry. Specifically, the flattening of cells grown on slides (considered the hallmark of cellular senescence) is measured as the decline in local intensity of DNA‐associated DAPI fluorescence (represented by maximal pixels). This change is paralleled by an increase in nuclear area. Thus, the ratio of mean intensity of maximal pixels to nuclear area provides a very sensitive morphometric biomarker for the degree of senescence. This analysis is combined with immunocytochemical detection of senescence markers, such as overexpression of cyclin kinase inhibitors (e.g., p21WAF1) and phosphorylation of ribosomal protein S6 (rpS6), a key marker associated with aging/senescence that is detected using a phospho‐specific antibody. These biomarker indices are presented in quantitative terms defined as a senescence index (SI), which is the fraction of the marker in test cultures relative to the same marker in exponentially growing control cultures. This system can be used to evaluate the anti‐aging potential of test agents by assessing attenuation of maximal senescence. As an example, the inclusion of berberine, a natural alkaloid with reported anti‐aging properties and a long history of use in traditional Chinese medicine, is shown to markedly attenuate the Mxt‐induced SI and phosphorylation of rpS6. The multivariate analysis of senescence markers by laser scanning cytometry offers a promising tool to explore the potential anti‐aging properties of a variety agents. Curr. Protoc. Cytom. 69:9.47.1‐9.47.10. © 2014 by John Wiley & Sons, Inc.

show abstract

Berberine suppresses gero-conversion from cell cycle arrest to senescence

Cited by 54 publications

References 89 publications

Geroconversion: irreversible step to cellular senescence

Geroconversion: irreversible step to cellular senescence

Attenuation of Replication Stress–Induced Premature Cellular Senescence to Assess Anti‐Aging Modalities

Contact Info

Product

Resources

About