Benzo[a]pyrene osteotoxicity and the regulatory roles of genetic and epigenetic factors: A review

Mo, Jiezhang; Au, Doris W.T.; Guo, Jiahua; Winkler, Christoph; Kong, Richard Yuen-Chong; Seemann, Frauke

doi:10.1080/10643389.2021.1915052

Cited by 20 publications

(54 citation statements)

References 189 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The consequences of environmental pollution with B[ a ]P/PAHs can thus be serious and may need reassessment. Mo et al [ 142 ], in a review, suggested that transgenerational inheritance of osteotoxicity in fish, induced by ancestral exposure to B[ a ]P, is mediated by epigenetically deregulated bone miRNA/genes. Harmful effects of B[ a ]P on aquatic animals and their offspring have been confirmed by subsequent studies.…”

Section: Adverse Effects Observed In In Vitro and In Vivo Studiesmentioning

confidence: 99%

Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Bukowska

Mokra

Michałowicz

2022

IJMS

156

View full text Add to dashboard Cite

Benzo[a]pyrene (B[a]P) is the main representative of polycyclic aromatic hydrocarbons (PAHs), and has been repeatedly found in the air, surface water, soil, and sediments. It is present in cigarette smoke as well as in food products, especially when smoked and grilled. Human exposure to B[a]P is therefore common. Research shows growing evidence concerning toxic effects induced by this substance. This xenobiotic is metabolized by cytochrome P450 (CYP P450) to carcinogenic metabolite: 7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE), which creates DNA adducts, causing mutations and malignant transformations. Moreover, B[a]P is epigenotoxic, neurotoxic, and teratogenic, and exhibits pro-oxidative potential and causes impairment of animals’ fertility. CYP P450 is strongly involved in B[a]P metabolism, and it is simultaneously expressed as a result of the association of B[a]P with aromatic hydrocarbon receptor (AhR), playing an essential role in the cancerogenic potential of various xenobiotics. In turn, polymorphism of CYP P450 genes determines the sensitivity of the organism to B[a]P. It was also observed that B[a]P facilitates the multiplication of viruses, which may be an additional problem with the widespread COVID-19 pandemic. Based on publications mainly from 2017 to 2022, this paper presents the occurrence of B[a]P in various environmental compartments and human surroundings, shows the exposure of humans to this substance, and describes the mechanisms of its toxicity.

show abstract

Section: Adverse Effects Observed In In Vitro and In Vivo Studiesmentioning

confidence: 99%

Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Bukowska

Mokra

Michałowicz

2022

IJMS

156

View full text Add to dashboard Cite

show abstract

“…Parental exposure to BaP has been known to induce bone deformities in unexposed offspring [ 6–9 ]; however, no BaP has been detected in the F1 embryos [ 8 ]. BaP-induced changes in germ cell DNA methylation were proposed as one of the epigenetic mechanisms responsible for cross- and transgenerational phenotype transmission (reviewed in 10 ), which has been corroborated by reduced DNA methyltransferase activity upon BaP exposure in zebrafish (F0) and mice (F1), as well as altered offspring methylation profiles [ 11 , 12 ]. Furthermore, DNA methylation profiles were investigated as potential genomic markers of environmental BaP exposures and may indicate the intergenerational inheritance of phenotype impairments [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…A growing body of evidence is suggesting that parental BaP exposure is inducing systemic impairment manifested as reproductive toxicity, cardiotoxicity, osteotoxicity, and neurotoxicity (reviewed in 10 ). Although several studies have covered multiple biological levels to understand ancestral BaP-induced osteotoxicity, the impacts of cross-generational neurotoxicity have only been reported at the organism level.…”

Section: Introductionmentioning

confidence: 99%

Assessment of parental benzo[a]pyrene exposure-induced cross-generational neurotoxicity and changes in offspring sperm DNA methylome in medaka fish

Wan

et al. 2022

Environmental Epigenetics

Self Cite

View full text Add to dashboard Cite

Previous studies have revealed that DNA methylation changes could serve as potential genomic markers for environmental benzo[a]pyrene (BaP) exposure and intergenerational inheritance of various physiological impairments (e.g., obesity and reproductive pathologies). As a typical aromatic hydrocarbon pollutant, direct BaP exposure has been shown to induce neurotoxicity. To unravel the inheritance mechanisms of the BaP-induced bone phenotype in freshwater medaka, we conducted whole-genome bisulfite sequencing of F1 sperm and identified 776 differentially-methylated genes (DMGs). Ingenuity Pathway Analysis (IPA) revealed DMGs were significantly enriched in pathways associated with neuronal development and function. Therefore, it was hypothesized that parental BaP exposure (1 μg/L, 21 days) causes offspring neurotoxicity. Furthermore, the possibility for sperm methylation as an indicator for a neurotoxic phenotype was investigated. The F0 adult brains and F1 larvae were analyzed for BaP-induced direct and inherited toxicity. Acetylcholinesterase activity was significantly reduced in the larvae, together with decreased swimming velocity. Molecular analysis revealed that the marker genes associated with neuron development and growth (alpha1-tubulin, mbp, syn2a, shh, and gap43) as well as brain development (dlx2, otx2, and krox-20) were universally downregulated in the F1 larvae (3-days post-hatching). While parental BaP exposure at an environmentally relevant concentration could induce neurotoxicity in the developing larvae, the brain function of the exposed F0 adults was unaffected. This indicates that developmental neurotoxicity in larvae may result from impaired neuronal development and differentiation, causing delayed brain growth. The present study demonstrates that the possible adverse health effects of BaP in the environment are more extensive than currently understood. Thus, the possibility of multigenerational BaP toxicity should be included in environmental risk assessments.

show abstract

“…Among over 400 carcinogenic PAHs and their derivates, benzo[a]pyrene (BaP) shows the strongest carcinogenicity [ 29 , 30 ]. It is a five-ring polycyclic aromatic hydrocarbon with a negative impact on human health, including teratogenicity, tumor formation, immunosuppression, osteotoxicity and hormonal effects [ 31 , 32 , 33 ]. The highest content of BaP was detected in parsley from Site 3 (46.53 ng/g FW), while its content in parsley collected from Site 2 was under the detection limit of the applied chromatographic method.…”

Section: Resultsmentioning

confidence: 99%

Determination of Polycyclic Aromatic Hydrocarbon Content in Garden Herbal Plants Using Liquid Chromatographic Analysis (HPLC-FL)

Woźniak

Hoppe

Drzewiecka

2023

Plants

View full text Add to dashboard Cite

Polycyclic aromatic hydrocarbons (PAHs) are a group of chemical compounds generated as a result of the incomplete combustion of fossil fuels or wood. PAHs are known for their negative effect on living organisms, including teratogenic, carcinogenic and mutagenic activity. The objective of this study is to determine the contamination of three popular herbal species showing pro-health properties, i.e., lavender, parsley and mint, with polycyclic aromatic hydrocarbons, collected from three different backyard gardens in Poland. The concentration of PAHs in plant material was determined by high-performance liquid chromatography with a fluorescence detector (HPLC-FL). The concentration of eleven PAHs in plant material was determined with high-pressure liquid chromatography after extraction using the QuEChERS purification technique. Mint collected within an area of a mining and energy production complex (the city of Konin) was characterized by the highest Σ of 11 PAHs, equaled to 902.35 µg/g FW, with anthracene being the most abundant compound. However, it contained the lowest sum of PAHs, among all tested plants, with high carcinogenicity. Parsley from the city of Poznań showed the highest content of benzo[a]pyrene (BaP), showing the strongest carcinogenicity, while the highest value of BaP equivalent was calculated for mint collected in Konin. The obtained results suggest that the level and profile of plant contamination with PAHs depend on the species and the location of herb cultivation. In particular, mining and energy industry facilities are sources of PAHs, which contaminate plant material for further direct use or as bioactive herbal extracts.

show abstract

Benzo[a]pyrene osteotoxicity and the regulatory roles of genetic and epigenetic factors: A review

Cited by 20 publications

References 189 publications

Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Benzo[a]pyrene—Environmental Occurrence, Human Exposure, and Mechanisms of Toxicity

Assessment of parental benzo[a]pyrene exposure-induced cross-generational neurotoxicity and changes in offspring sperm DNA methylome in medaka fish

Determination of Polycyclic Aromatic Hydrocarbon Content in Garden Herbal Plants Using Liquid Chromatographic Analysis (HPLC-FL)

Contact Info

Product

Resources

About