Benthic-epiphytic dinoflagellates from the northern portion of the Mesoamerican Reef System

Almazán-Becerril, Antonio; Escobar-Morales, Sergio; Rosiles‐González, Gabriela; Valadez, Francisco

doi:10.1515/bot-2014-0093

Cited by 25 publications

(6 citation statements)

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“…Once in the laboratory, macroalgae samples were processed following the protocol by Reguera et al (2011) as follows: samples were vigorously shaken (2 min) to detach the cells. Macroalgae were removed, and the water suspension was filtered and washed with filtered seawater (TCLP glass fiber filters, 0.7 µm; Pall Laboratory, Port of Mexico and the Yucatán Peninsula (Okolodkov et al 2007, Almazán-Becerril et al 2015.…”

Section: Collection and Preliminary Handling Of Macroalgae Samplesmentioning

confidence: 99%

“…However, the seagrasses Halodule wrightii Ascherson 1868 and Syringodium filiforme Kützing 1860 are also dominant species in the Gulf of Mexico, along with mainly green macroalgal species, such as Caulerpa ashmeadii Harvey 1858, C. paspaloides (Bory) Greville 1030, C. prolifera (Forsskål) J. V. Lamouroux 1809, Halimeda incrassata (J. Ellis) J. V. Lamouroux 1816, and Udotea flabellum (J. Ellis & Solander) M. Howe 1904 (Okolodkov et al 2007). In the Mexican Caribbean, Coolia species have been found associated with Amphiroa J. V. Lamouroux, 1812 and Dictyota, J. V. Lamouroux, 1809; particularly, C. tropicalis is a very conspicuous species on a variety of macroalgae along the coast (Almazán-Becerril et al 2015). In Bahía de La Paz, southern Gulf of California, Coolia malayensis was isolated from Sargassum horridum Setchell & N. L. Gardner 1924(Sepúlveda-Villarraga 2017.…”

Section: Collection and Preliminary Handling Of Macroalgae Samplesmentioning

confidence: 99%

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Morphological and molecular characterization of the genus <italic>Coolia</italic> (Dinophyceae) from Bahía de La Paz, southwest Gulf of California

Morquecho¹,

Gárate-Lizárraga²,

Gu³

2022

Algae

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The genus Coolia A. Meunier 1919 has a global distribution and is a common member of epiphytic dinoflagellate assemblages in neritic ecosystems. Coolia monotis is the type species of the genus and was the only known species for 76 years. Over the past few decades, molecular characterization has unveiled two species complexes that group morphologically very similar species, so their limits are often unclear. To provide new knowledge on the biogeography and species composition of the genus Coolia, 16 strains were isolated from Bahía de La Paz, Gulf of California. The species were identified by applying morphological and molecular approaches. The morphometric characteristics of all isolated Coolia species were consistent with the original taxa descriptions. Phylogenetic analyses (large subunit [LSU] rDNA D1 / D2 and internal transcribed spacer [ITS] 1 / 5.8S / ITS2) revealed a species assemblage comprising Coolia malayensis, C. palmyrensis, C. tropicalis, and the C. cf. canariensis lineage. This is the first report of Coolia palmyrensis and C. cf. canariensis in Mexico and C. tropicalis in the Gulf of California. Our results strengthen the biogeographical understanding of these potentially harmful epiphytic dinoflagellate species.

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Section: Collection and Preliminary Handling Of Macroalgae Samplesmentioning

confidence: 99%

Section: Collection and Preliminary Handling Of Macroalgae Samplesmentioning

confidence: 99%

Morphological and molecular characterization of the genus <italic>Coolia</italic> (Dinophyceae) from Bahía de La Paz, southwest Gulf of California

Morquecho¹,

Gárate-Lizárraga²,

Gu³

2022

Algae

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“…Amphidiniella sp. Núñez-Vázquez, 2005;Okolodkov et al, 2007Okolodkov et al, , 2014Okolodkov et al, , 2016Gárate-Lizárraga, 2012, 2020Hernández-Castro, 2017;Aguilar-Trujillo et al, 2014Almazán-Becerril et al, 2015;Martínez-Cruz et al, 2015 Okolodkov & Gárate-Lizárraga, 2006;Okolodkov et al, 2007Okolodkov et al, , 2014Morquecho et al, 2016;Almazán-Becerril et al, 2016b Okolodkov et al, 2007Okolodkov et al, , 2014Okolodkov et al, , 2016Aguilar-Trujillo et al, 2014Almazán-Becerril et al, 2015, 2016bIrola-Sansores et al, 2018;Méndez-Torres, 2019 The first sequence data for genus Amphidinium from Mexico was provided from the study to determine the toxigenicity and cytotoxic effects of A. operculatum in culture (Mejía-Camacho et al, 2021). The identification of Amphidinium strain AA60 as A. operculatum was confirmed by SEM and sequencing of the LSU and ITS regions of rDNA.…”

Section: Referencesmentioning

confidence: 99%

“…The primary focus on a handful of key BHAB species has been necessary because of resource and personnel limitations, but has led to a relative neglect of cryptic, rare and non-BHAB taxa -hence a likely underestimate of diversity of benthic dinoflagellates in Mexican waters. In most cases, taxonomic studies have simply corroborated original descriptions based on morphological traits and strengthened local knowledge for accurate species identification (Cortés-Altamirano & Sierra-Beltrán, 2003;Hernández-Becerril & Almazán-Becerril, 2004;Almazán-Becerril et al, 2015, 2016a. A few more profound taxonomic investigations have indeed contributed to reclassification of genera or species within newly created genera or to resolving species complexes (Tarazona-Janampa et al, 2020;Cembella et al, 2021).…”

Section: Speciesmentioning

confidence: 99%

A review on marine benthic dinoflagellates in Mexico

Okolodkov¹

2022

Hidrobiológica

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Background: Mexican studies on marine benthic dinoflagellates (MBD) began in 1942 from the offshore region of Oaxaca, based on water column samples. Subsequently, in ten Mexican maritime states, both epibenthos and plankton samples have been collected, species have been cultured, and field and laboratory studies have been carried out. Goals: The objective of this contribution is to review the studies on MBD in Mexican waters for current status and future risk assessment. Methods: Available literature on MBD from Mexico published from 1942 to 2022 was analyzed. Results: A review of the studies on MBD is presented herein, subdivided into four sections: (1) taxonomic diversity (morphological and molecular), (2) benthic phycotoxin vectors and toxigenicity, (3) toxicity and (4) species interactions. A map of the location of field populations of MBD and a reference list of taxonomic and ecological studies on MBD in Mexico is provided. A taxonomic list of ca. 60 species, mainly of Prorocentrum, followed in number by Amphidinium, Gambierdiscus, Ostreopsis, Coolia, and Sinophysis is presented. This list is accompanied by scanning electron microscopic images of 15 species. Knowledge of the toxigenicity of MBD is scarce, hence a reference table of known toxigenic species in Mexico and their respective associated syndromes is included. Conclusions: Studies on MBD in Mexico have slowly increased, probably due to the availability of a greater number of cultured strains, as well as to more extensive international collaborations. Confirmed links between benthic harmful algal bloom species and events associated with the etiology of toxic syndromes are rarely known in Mexico.

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“…Benthic dinoflagellate species are quite well described morphologically from Latin America (reviewed in Durán-Riveroll et al (2019) but there are many gaps in known phylogeographical distribution, few confirmed records of cell toxicity or toxin composition and a paucity of molecular confirmation of species identities and phylogenetic relationships. This poor state of knowledge also applies to benthic Prorocentrum species although members of the genus have been reported and described from both the Pacific [for references, see Okolodkov and Gárate-Lizárraga (2006); Gárate-Lizárraga et al (2007)], and Gulf of Mexico and Caribbean coasts of Mexico (Okolodkov et al, 2007(Okolodkov et al, , 2014Barón-Campis et al, 2014;Almazán-Becerril et al, 2015;Aguilar-Trujillo et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Phylogeography and Diversity Among Populations of the Toxigenic Benthic Dinoflagellate Prorocentrum From Coastal Reef Systems in Mexico

et al. 2021

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The marine dinoflagellate genus Prorocentrum Ehrenberg comprises many species occupying primarily benthic or epiphytic habitats, particularly in tropical and sub-tropical waters. Despite concerted efforts to establish phylogenetic associations, there remain unresolved issues in defining morphospecies and membership in species complexes. The study described herein addressed the inter- and infraspecific relationships of members of the Prorocentrum lima and Prorocentrum hoffmannianum species complexes (PLSC and PHSC, respectively) by applying multivariate approaches in morphotaxonomy, molecular phylogenetics and chemodiversity to establish affinities among multiple clonal isolates. Morphotaxonomic analysis showed consistency with classical morphospecies descriptors, and high variability in cell size and dimensions, but did not challenge current species complex concepts. Phylogenetic analysis of ITS/5.8S rDNA sequences from isolates from the Gulf of California, Caribbean Sea, and Gulf of Mexico coasts compared with archived global GenBank sequences served to define five consistent clades with separation of the PLSC and PHSC. Secondary structure modeling of ITS2 rRNA variation based on compensatory base changes (CBC) was effective in resolving details of the respective species complexes and even indicated putative incipient or cryptic speciation due to potential hybridization barriers. This study represents the largest (n = 67 isolates) chemodiversity analysis of polyketide-derived toxins associated with diarrheic shellfish poisoning (DSP) from a benthic dinoflagellate genus. Relative composition of some analogs (OA, OA-D8, DTX1, DTX1a, and DTX1a-D8), including two new undescribed isomers, distinguished P. lima from P. hoffmannianum sensu lato, but without clear associations with substrate type or geographical origin. Although all P. lima and most (one exception) P. hoffmannianum were toxigenic, the total cell toxin content could not be linked at the species level. This research demonstrates that clonal chemodiversity in toxin composition cannot yet be effectively applied to define ecological niches or species interactions within local assemblages. Phylogenetic analysis of the ITS/5.8 rDNA, particularly when combined with secondary structure modeling, rather than only a comparison of LSU rDNA sequences, is a more powerful approach to identify cryptic speciation and to resolve species complexes within benthic dinoflagellate groups.

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Benthic-epiphytic dinoflagellates from the northern portion of the Mesoamerican Reef System

Cited by 25 publications

References 0 publications

Morphological and molecular characterization of the genus <italic>Coolia</italic> (Dinophyceae) from Bahía de La Paz, southwest Gulf of California

Morphological and molecular characterization of the genus <italic>Coolia</italic> (Dinophyceae) from Bahía de La Paz, southwest Gulf of California

A review on marine benthic dinoflagellates in Mexico

Phylogeography and Diversity Among Populations of the Toxigenic Benthic Dinoflagellate Prorocentrum From Coastal Reef Systems in Mexico

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