Benefits of inoculation of the common bean ( Phaseolus vulgaris ) crop with efficient and competitive Rhizobium tropici strains

Hungría, Mariangela; Campo, R. J.; Mendes, I. C.

doi:10.1007/s00374-003-0682-6

Cited by 160 publications

(136 citation statements)

References 23 publications

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“…It confirms that traits related to biological N 2 fixation are more responsive to soil P supply than plant growth (Almeida et al, 2000;Araújo & Teixeira, 2000). Even control plants showed a strong nodulation (Figure 1b), which reinforces the argument that the common bean is a promiscuous host regarding native soil rhizobia (Hungria et al, 2003). The addition of mineral N strongly reduced nodulation at both soil P levels (Figure 1b) since the Phaseolus-Rhizobium symbiosis is quite sensitive to soil nitrate (Westermann et al, 1981;Leidi & Rodríguez-Navarro, 2000).…”

Section: Plant Growthsupporting

confidence: 79%

“…Therefore, in spite of the intense nodulation of control plants (Figure 1b), the inoculation of selected rhizobia strains contributed to improve N 2 fixation in common bean. Hungria et al (2003) also verified that inoculation with selected strains increased N 2 fixation of field-grown common beans, expressed by higher N content in shoots and higher percentage of N as ureides. Higher soil P level increased the %Ndfa in bean leaves (Table 3).…”

Section: N Isotope Dilutionsupporting

confidence: 61%

“…The common bean (Phaseolus vulgaris L.) has been considered of low biological N 2 fixation capacity as compared to other grain legumes, which is attributed to the poor soil conditions where the crop is usually grown, the susceptibility of the species to nutritional and environmental stresses, the short vegetative period available for N 2 fixation, the sensitivity of the symbiosis to soil nitrate, and the competition from indigenous but often ineffective soil rhizobia (Piha & Munns, 1987;Leidi & Rodríguez-Navarro, 2000;Graham et al, 2003;Hungria et al, 2003). Nevertheless, ranges of biologically fixed N 2 in fieldgrown bean lines were reported as from 25 to 65 kg ha -1 (Ruschel et al, 1982) or 40 to 125 kg ha -1 (Rennie & Kemp, 1983) in shoots at physiological maturity, or from 21 to 44 kg ha -1 in grains (Pereira et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

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Seeds enriched with phosphorus and molybdenum improve the contribution of biological nitrogen fixation to common bean as estimated by 15n isotope dilution

Chagas¹,

Araújo

Alves

et al. 2010

Rev. Bras. Ciênc. Solo

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SUMMARYSeeds with a high concentration of P or Mo can improve the growth and N accumulation of the common bean (Phaseolus vulgaris L.), but the effect of enriched seeds on biological N 2 fixation has not been established yet. This study aimed to evaluate the effect of seeds enriched with P and Mo on growth and biological N 2 fixation of the common bean by the 15 N isotope dilution technique. An experiment was carried out in pots in a 2 x 3 x 2 x 2 factorial design in randomized blocks with four replications, comprising two levels of soil applied P (0 and 80 mg kg -1 ), three N sources (without N, inoculated with rhizobia, and mineral N), two seed P concentrations (low and high), and two seed Mo concentrations (low and high). Non-nodulating bean and sorghum were used as non-fixing crops. The substrate was 5.0 kg of a Red Latosol (Oxisol) previously enriched with 15 N and mixed with 5.0 kg of sand. Plants were harvested 41 days after emergence. Seeds with high P concentration increased the growth and N in shoots, particularly in inoculated plants at lower applied P levels. Inoculated plants raised from high P seeds showed improved nodulation at both soil P levels. Higher soil P levels increased the percentage of N derived from the atmosphere (%Ndfa) in bean leaves. Inoculation with the selected strains increased the %Ndfa. High seed P increased the %Ndfa in inoculated plants at lower soil P levels. High seed Mo increased the %Ndfa at lower soil P levels in plants that did not receive inoculation or mineral N. It is concluded that high seed P concentration increases the growth, N accumulation RESUMO: SEMENTES ENRIQUECIDAS COM FÓSFORO E MOLIBDÊNIO

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Section: Plant Growthsupporting

confidence: 79%

Section: N Isotope Dilutionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

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Seeds enriched with phosphorus and molybdenum improve the contribution of biological nitrogen fixation to common bean as estimated by 15n isotope dilution

Chagas¹,

Araújo

Alves

et al. 2010

Rev. Bras. Ciênc. Solo

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“…A maioria dos solos com histórico de cultivo de feijão contém rizóbios nativos que podem interferir no estabelecimento e na eficácia das estirpes inoculadas, reduzindo as respostas à inoculação (Vlassak et al, 1996;Vargas et al, 2000;Raposeiras et al, 2006 (Mostasso et al, 2002;Raposeiras et al, 2006), na Região Sul Hungria et al, 2003;Stocco et al, 2008) e na Região Sudeste (Soares et al, 2006;Lombardi et al, 2009;Torres et al, 2009). Além dessas estirpes de Rhizobium, grande número de isolados classificados como Sinorhizobium sp.…”

Section: Introductionunclassified

Resposta do Feijoeiro Comum à Inoculação com Rizóbio e Suplementação com Nitrogênio Mineral em Dois Biomas Brasileiros

Brito

Pacheco

Filho

et al. 2015

Rev. Bras. Ciênc. Solo

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O feijoeiro comum (Phaseolus vulgaris L.) pode se beneficiar da fixação biológica de N2, mas respostas inconsistentes da cultura à inoculação com rizóbio indicam a necessidade de aplicação de N mineral complementar. Este trabalho teve por objetivo avaliar a resposta do feijoeiro à inoculação com rizóbio, associada à suplementação com N mineral, nos biomas Cerrado e Mata Atlântica. Foram conduzidos quatro experimentos de campo, dois em Santo Antônio de Goiás, GO, um em Valença, RJ e um em Macaé, RJ, onde a inoculação com estirpes comerciais de rizóbio foi comparada à inoculação com a estirpe BR 923 de Sinorhizobium sp., à adubação com N mineral e à suplementação com N na semeadura e em cobertura. A avaliação da população nativa de rizóbio indicou 105 células g-1 no solo na área experimental em Goiás, anteriormente cultivada com feijão, e 102células g-1 em Valença, anteriormente mantida com pastagem. Nos dois experimentos em Goiás, o rendimento de grãos, da ordem de 2.100 kg ha-1, não diferiu entre os tratamentos testemunha absoluta, inoculação com rizóbio ou aplicação de 120 kg ha-1 de N. Em Valença, a inoculação com estirpes comerciais forneceu rendimentos da cultivar Ouro Negro superiores à testemunha absoluta, na ausência de adubação de cobertura; na presença de 40 kg ha-1 de N em cobertura, a inoculação com rizóbio proporcionou rendimento de 3.420 kg ha-1, superior aos demais tratamentos. Na média das diferentes fontes de N na semeadura, a adubação de cobertura aumentou a produção de grãos de 2.367 para 2.542 kg ha-1. Em Macaé, em solo com alto teor de matéria orgânica, os maiores rendimentos foram obtidos com inoculação das estirpes comerciais associada a 40 kg ha-1 de N em cobertura, com efeito deletério da adubação de 80 kg ha-1 de N no plantio. Concluiu-se que em áreas sem cultivo prévio de feijão, a inoculação com estirpes comerciais de rizóbio aumentou o rendimento de grãos, em particular quando associada à adubação de cobertura com N.

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“…Nevertheless, further research to isolate new strains with genetic diversity and nitrogen fi xation effi ciency has been carried out. In this sense, Mostasso et al (2002) and Hungria et al (2003) evaluated the H12 strain (SEMIA 4088), and concluded that it was effi cient and competitive, providing high yield potential to common beans. Raposeiras et al (2006) and Soares et al (2006) carried out studies on agronomic effi ciency of new Rhizobium strains in common bean, in different Brazilian environments, and identifi ed only strains that made possible productivities similar to the obtained with CIAT-899 strain inoculation.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Gonzalez

Campanharo

Lemos

2008

Pesq. agropec. bras.

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-This study aimed to genetically characterize four new Rhizobium strains, and to evaluate their nodulation and fi xation capacity compared to commercial strains and to native rhizobia population of a Brazilian Rhodic Hapludox. Two experiments were carried out in randomized blocks design, under greenhouse conditions, in 2007. In the fi rst experiment, the nodulation and nitrogen fi xation capacity of new strains were evaluated, in comparison to the commercial strains CIAT-899 and PRF-81 and to native soil population. It was carried out in plastic tubes fi lled with vermiculite. DNA extractions and PCR sequencing of the intergenic space were made from the isolated pure colonies, in order to genetically characterize the strains and the native rhizobia population. In the second experiment, the nodulation and productivity of common beans Perola cultivar were determined, with the use of evaluated strains, alone or in mixture with PRF-81 strain. It was carried out in pots fi lled with soil. The native soil population was identifi ed as Rhizobium sp. and was ineffi cient in nitrogen fi xation. Three different Rhizobium species were found among the four new strains. The LBMP-4BR and LBMP-12BR new strains are among the ones with greatest nodulation and fi xation capacity and exhibit differential responses when mixed to PRF-81.Index terms: Phaseolus vulgaris, biological nitrogen fi xation, intergenic space, phylogeny, sequencing, soil rhizobia population. Caracterização genética e capacidade de fi xação de nitrogênio de estirpes de Rhizobium em feijoeiroResumo -O objetivo deste trabalho foi a caracterização genética de quatro novas estirpes de Rhizobium e a avaliação de sua capacidade de fi xação de N 2 e nodulação, comparadas a estirpes comerciais e à população nativa de rizóbios de um Latossolo Vermelho. Dois experimentos foram conduzidos em blocos ao acaso, em casa de vegetação. No primeiro experimento, conduzido em tubetes com vermiculita, avaliaram-se a nodulação e a capacidade de fi xação das novas estirpes, em comparação com as estirpes comerciais CIAT-899 e PRF-81 e com a população nativa do solo. Das colônias puras isoladas, extraiu-se o DNA genômico e realizou-se o seqüenciamento do espaço intergênico, para a caracterização genética das estirpes e da população nativa de rizóbios. O segundo experimento foi realizado em vasos com solo, para determinação da produtividade e da nodulação do feijoeiro, cultivar Pérola, com o uso das estirpes isoladamente ou em mistura com a PRF-81. A população nativa do solo foi identifi cada como Rhizobium sp. e se mostrou inefi ciente na fi xação de nitrogênio. Foram encontradas três espécies de Rhizobium entre as quatro novas estirpes. As estirpes LBMP-4BR e LBMP-12BR estão entre as que têm maior capacidade de nodulação e fi xação de N 2 , e apresentam respostas diferenciadas quando misturadas à PRF-81.Termos para indexação: Phaseolus vulgaris, fi xação biológica de nitrogênio, espaço intergênico, fi logenia, seqüenciamento, população nativa de rizóbios.

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Benefits of inoculation of the common bean ( Phaseolus vulgaris ) crop with efficient and competitive Rhizobium tropici strains

Cited by 160 publications

References 23 publications

Seeds enriched with phosphorus and molybdenum improve the contribution of biological nitrogen fixation to common bean as estimated by 15n isotope dilution

Seeds enriched with phosphorus and molybdenum improve the contribution of biological nitrogen fixation to common bean as estimated by 15n isotope dilution

Resposta do Feijoeiro Comum à Inoculação com Rizóbio e Suplementação com Nitrogênio Mineral em Dois Biomas Brasileiros

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

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