volume 38, issue 5, P1281-1288 2004
DOI: 10.1016/j.watres.2003.10.052
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Matthew B. Mesarch, Cindy H. Nakatsu, Loring Nies

Abstract: The objective of this work was to test a molecular genetic method for in situ monitoring of aerobic benzene, toluene, and xylene (BTX) biodegrading microorganisms. Catechol 2,3-dioxygenase (C23DO) genes occur in bacteria that biodegrade benzene, toluene, xylenes, phenol, biphenyl, and naphthalene. A competitive quantitative polymerase chain reaction (QC-PCR) technique using a single set of primers specific for an entire subfamily of C23DO genes was recently developed. To determine whether bacteria containing t…

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