Behavioral Responses of Javanese Medaka (Oryzias Javanicus) Versus Zebrafish (Danio Rerio) in Open Field Test.

Sataa, Nurul Syafida Asma’ Mohd; Bakar, Noraini Abu; Hodin, Nur Atikah Saleh; Ramlan, Nurul Farhana; Ibrahim, Wan Nazihah Wan

doi:10.21203/rs.3.rs-50649/v1

Cited by 5 publications

(3 citation statements)

References 58 publications

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“…In terms of their differences from the zebrafish, all of the medaka fishes exhibited a relatively lower locomotor activity than the zebrafish most of the time. This result is somewhat similar to a prior study in another species of medaka ( Oryzias javanicus ), which found that the medaka fish were passive compared to the D. rerio who were more active and aggressive [ 43 ]. Moreover, a high thigmotaxis level, a preference of animals towards the periphery of a novel arena and avoiding the center area, during the test was displayed by all of the medaka fish [ 44 ].…”

Section: Discussionsupporting

confidence: 90%

Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics

Audira

Siregar

Chen

et al. 2021

IJMS

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Recently, medaka has been used as a model organism in various research fields. However, even though it possesses several advantages over zebrafish, fewer studies were done in medaka compared to zebrafish, especially with regard to its behavior. Thus, to provide more information regarding its behavior and to demonstrate the behavioral differences between several species of medaka, we compared the behavioral performance and biomarker expression in the brain between four medaka fishes, Oryzias latipes, Oryzias dancena, Oryzias woworae, and Oryzias sinensis. We found that each medaka species explicitly exhibited different behaviors to each other, which might be related to the different basal levels of several biomarkers. Furthermore, by phenomics and genomic-based clustering, the differences between these medaka fishes were further investigated. Here, the phenomic-based clustering was based on the behavior results, while the genomic-based clustering was based on the sequence of the nd2 gene. As we expected, both clusterings showed some resemblances to each other in terms of the interspecies relationship between medaka and zebrafish. However, this similarity was not displayed by both clusterings in the medaka interspecies comparisons. Therefore, these results suggest a re-interpretation of several prior studies in comparative biology. We hope that these results contribute to the growing database of medaka fish phenotypes and provide one of the foundations for future phenomics studies of medaka fish.

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Section: Discussionsupporting

confidence: 90%

Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics

Audira

Siregar

Chen

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…In terms of their differences from zebrafish, all medaka fishes exhibited a relatively lower locomotor activity than zebrafish most of the time. This result is somewhat similar to a prior study in another species of medaka (O. javanicus), which found that the medaka fish were passive compared to D. rerio that were more active and aggressive [37]. Moreover, a high thigmotaxis level, a preference of animals towards the periphery of a novel arena and avoiding the center area, during the test was displayed by all medaka fish [38].…”

Section: Discussionsupporting

confidence: 90%

Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics

Audira¹,

Siregar²,

Chen³

et al. 2021

Preprint

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Recently, medaka has been used as a model organism in various research fields. However, even though it possesses several advantages over zebrafish, fewer studies were done in medaka than zebrafish, especially its behaviour. Thus, to provide more information regarding its behaviour and to demonstrate the be-havioural differences between several species of medaka, we compared the behavioural performance and biomarker expression in the brain between four medaka fishes, which were Oryzias latipes, O. dancena, O. woworae, and O. sinensis. From the results, we found that each medaka species explicitly exhib-ited different behaviours to each other, which might be related to the different basal levels of several biomarkers. Furthermore, by phenomics and ge-nomic-based clustering, the differences between these medaka fishes were further investigated. Interestingly, even though both phenomics and ge-nomic-based clustering showed some resemblances to each other in terms of the interspecies relationship between medaka and zebrafish, however, in the medaka interspecies comparisons, this similarity was not displayed. Therefore, these results suggest a re-interpretation of several prior studies in comparative biology. We hope that these results contribute to the growing database of medaka fish phenotypes and provide one of the foundations for future phe-nomics studies of medaka fish.

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“…The open eld test (OFT) was utilized to analyze the swimming behavior and locomotor activity of adult zebra sh as described by Sattaa et al, [29]. The OFT is made up of plexiglass (dimensions 30 m height X 30 cm width X10 cm height) containing 5L of water at 28•C.…”

Section: Open Eld Testmentioning

confidence: 99%

Pre-Treatment with Quercetin Prevents Dementia in Streptozotocin Treated Zebrafish via Modulation of Neuroinflammation and Oxidative Stress

Pradhan

Devi

Sharma

et al. 2021

Preprint

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Aim: To examine the protective outcome of quercetin against streptozotocin induced dementia in adult zebrafish. Materials and method: In this study, adult zebrafish (weighing 470-530 mg) were subjected to the streptozotocin administration (300 mg/kg; i.p) followed by a quercetin (50 & 100mg/kg i.p) pre-treatment before 24 hr of streptozotocin administration followed by blood glucose level measurement, behavioral parameters (light-dark test, novel diving test, open field test and T-maze test), biochemical parameters (lipid peroxidase, reduced glutathione, nitrite and AChEs activity), molecular and histopathological analysis. Results: In light-dark test, streptozotocin treated zebrafish shown their preference in dark compartment as compared to normal group. In novel diving tank, streptozotocin zebrafish spent less time and decrease in total number of entries to the top zone when compared to the normal group. In the T-maze test, streptozotocin treated zebrafish has shown significantly more time spent in unfavorable zone and less time spent in favorable zone with increase in total latency as compared to normal group. However, in the open field test no significant effect was seen among any group. Moreover, pre-treatment with quercetin (50 mg/kg and 100 mg/kg) significantly ameliorated the memory deficits in all the behavioral, biochemical and molecular parameters as compared to animals treated with streptozotocin. Additionally, results of histopathology studies showed less disruption in neuronal cells in quercetin treated group when compared to STZ treated animals.Conclusion: Pre-treatment with quercetin ameliorates streptozotocin induced dementia in adult zebrafish by preventing oxidative stress and neuroinflammation.

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Behavioral Responses of Javanese Medaka (Oryzias Javanicus) Versus Zebrafish (Danio Rerio) in Open Field Test.

Cited by 5 publications

References 58 publications

Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics

Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics

Interspecies Behavioral Variability of Medaka Fish Assessed by Comparative Phenomics

Pre-Treatment with Quercetin Prevents Dementia in Streptozotocin Treated Zebrafish via Modulation of Neuroinflammation and Oxidative Stress

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