2016
DOI: 10.1097/wnr.0000000000000554
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Abstract: In cranial and spinal nerve ganglia, both axotomized primary sensory neurons without regeneration (axotomy-nonregenerative neurons) and spared intact primary sensory neurons adjacent to axotomized neurons (axotomy-spared neurons) have been definitely shown to participate in pain transmission in peripheral neuropathic pain states. However, whether axotomized primary sensory neurons with regeneration (axotomy-regenerative neurons) would be integral components of neural circuits underlying peripheral neuropathic … Show more

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Cited by 6 publications
(14 citation statements)
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“…For imaging convenience and scalability, we utilized optical clearing-assisted, wide-field epifluorescent imaging and 3-D blind deconvolution, which were shown in previous studies by our group and others to enable high-contrast 3-D visualization of thick tissue samples comparable to confocal imaging (10,12). All of the fluorescently immunolabeled free-floating thick tissue sections were imaged using a fully motorized, Olympus IX-83 widefield (WF) epifluorescence microscope with a DP80 CCD camera under the monochromatic mode controlled by Olympus cellSens Dimension software (Olympus; Tokyo, Japan) (10).…”
Section: Methodsmentioning
confidence: 99%
“…In brief, after extensive washing with PBS-G (0.3 M glycine in 0.01 M PBS, pH 7.4) at RT, free-floating thick tissue sections were preconditioned for tissue loosening: ( i ) skin: neat DMSO for 15 min to loosen collagen structures in the upper dermis (UD) and dense cellular networks in the epidermis (10); ( ii ) spinal cord: antigen retrieval with sodium citrate solution (0.01 M sodium citrate, 0.05% Tween-20, pH 6.0) at 80C for 45 min to improve antigenic reactivity and antibody penetration (9,11); and ( iii ) sciatic nerve: thermally-assisted delipidation with 0.2 M sodium dodecyl sulfate (SDS) (in PBS, pH 9.0) at 37C for 3 h (7). After extensive washing with PBST (0.2% Tween-20 in 0.01 M PBS, pH 7.4) at RT, thick tissue sections were then permeabilized with PBS-Tx (0.3% Triton X-100 in 0.01 M PBS, pH 7.4): 4 15 min for skin and spinal cords, and 6 15 min for sciatic nerves.…”
Section: Methodsmentioning
confidence: 99%
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