Behavior of Tissue-Engineered Skin

Muhart, Michelle; McFalls, Susan; Kirsner, Robert S.; Elgart, George W.; Kerdel, Francisco A.; Sabolinski, Michael L.; Hardin-Young, Janet; Eaglstein, William H.

doi:10.1001/archderm.135.8.913

Cited by 88 publications

(23 citation statements)

References 18 publications

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“…1A, 1B). Structurally, engineered and human skin have been shown to be similar in use42 and these simplified tissue models have retained many important skin functions. We compared both the structure and the spectral features of the common tissue sub-types between human and engineered skin.…”

Section: Resultsmentioning

confidence: 99%

Characterization of tumor progression in engineered tissue using infrared spectroscopic imaging

Kong

Reddy

Bhargava

2010

Analyst

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Engineered tissues can provide models for imaging and disease progression and the use of such models is becoming increasingly prevalent. While structural characterization of these systems is documented, a combination of biochemical and structural knowledge is often helpful. Here, we apply Fourier transform infrared (FT-IR) spectroscopic imaging to examine an engineered tissue model of melanoma. We first characterize the biochemical properties and spectral changes in different layers of growing skin. Second, we introduce malignant melanocytes to simulate tumor formation and growth. Both cellular changes associated with tumor formation and growth can be observed. In particular, chemical changes associated with tumor-stromal interactions are observed during the course of tumor growth and appear to influence a 50-100 μm region. The development of this analytical approach combining engineered tissue with spectroscopy, imaging and computation will allow for quality control and standardization in tissue engineering and novel scientific insight in cancer progression.

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Section: Resultsmentioning

confidence: 99%

Characterization of tumor progression in engineered tissue using infrared spectroscopic imaging

Kong

Reddy

Bhargava

2010

Analyst

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“…On the other hand, a skin equivalent Bell et al [6] devised in 1983 has been clinically utilized as a skin substitute for acute and chronic wound closure [13,22,28]. It is comparatively easy to handle and has the advantage of a higher take rate than cultured epithelial sheet.…”

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confidence: 99%

Three-Dimensional Culture of Keratinocytes and the Formation of Basement Membrane for Canine Footpad Substitute

Yamazoe

Miyamoto

Hikosaka

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. A pad equivalent for a dog was prepared as a substitute for the loss of footpad. In addition to the time course of formation on epidermal morphogenesis, we investigated expressions of α 6 integrin subunit as adhesive molecule, and laminin and type IV and VII collagens as extracellular matrices of basement membrane components. Epithelium of the pad equivalent was thick enough to be easily confirmed at 5 days at the air-liquid interface, but many creases appeared on it at 7 days, and it shrank at 10 and 14 days. Keratinocytes were increased in 4 to 5 cell layers at 1 day at the air-liquid interface, differentiating into basal cell layer. Granular and corneal cell layers were confirmed until 5 days, and maintained their shape at least until 14 days. Alpha 6 integrin was expressed at almost the same fluorescent intensity as native pad tissue at 1 day at the dermal-epidermal junction. Laminin and type IV collagen were intermittently expressed at 5 and 10 days, respectively, at the dermal-epidermal junction, and at 14 days the fluorescence showed almost the same intensity as native pad tissue. The expression of type VII collagen was discontinuous at 2 days at the dermal-epidermal junction, but remained as it was at 14 days. The present findings suggested that although the formation of anchoring fibrils in basement membrane was incomplete, the pad equivalent in the dog was reconstructed similar to a native pad by epidermal morphogenesis. KEY WORDS: air-liquid interface, canine, epidermis, footpad, keratinocyte.

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“…24–27 One study of 20 patients compared a living skin equivalent to autograft and polyurethane occlusive dressing for management of the STSG donor site and showed a living skin equivalent and autograft to be similar, and both superior to the polyurethane occlusive dressing. 28 Other case reports have reported various methods of autologous grafting of the donor site, without reporting complication rates or other statistics. 1,29 One case study describing an autologous grafting method used in 46 patients reported no complications in their cohort.…”

Section: Discussionmentioning

confidence: 99%

Back Grafting the Split-Thickness Skin Graft Donor Site

Goverman

Kraft

Fagan

et al. 2017

Journal of Burn Care & Research

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Split-thickness skin grafting is a useful method of wound repair in burn and reconstructive operations. However, skin grafts require a donor site injury that creates a secondary wound at risk for delayed wound healing. Though in young healthy patients such donor sites have minimal risk, patients with risk factors for delayed wound healing are more challenging. We present a method for graft donor site management that offers an alternative to healing by secondary intention for patients with higher risk of poor wound healing. In those patients considered to be at high risk for donor site healing complications, we chose to treat the donor site with a split-thickness skin graft, or “graft back” procedure. An additional graft is taken adjacent to the initial donor site, and meshed 4:1 to cover both donor sites at once. Out of the 17 patients who received this procedure, 1 patient had a complication from the procedure that did not require an operation, and all patients appear to have good functional and cosmetic outcomes. No patients had any graft loss or graft infection. Histologic analysis showed complete epithelialization of the back-grafted area. The graft back method converts an open wound to a covered wound and may result in decreased wound healing time, improved cosmetic outcomes, and fewer complications, particularly in patients where wound healing is a concern. Importantly, it seems to have minimal morbidity. More detailed prospective studies are needed to ensure no additional risk is incurred by this procedure.

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Behavior of Tissue-Engineered Skin

Cited by 88 publications

References 18 publications

Characterization of tumor progression in engineered tissue using infrared spectroscopic imaging

Characterization of tumor progression in engineered tissue using infrared spectroscopic imaging

Three-Dimensional Culture of Keratinocytes and the Formation of Basement Membrane for Canine Footpad Substitute

Back Grafting the Split-Thickness Skin Graft Donor Site

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