Bean Golden Mosaic Geminivirus Type II Isolates from the Dominican Republic and Guatemala: Nucleotide Sequences, Infectious Pseudorecombinants, and Phylogenetic Relationships

Faria, J. C.; Gilbertson, R. L.; Hanson, Stephen F.; Morales, Francisco J.; Ahlquist, Paul; Loniello, Amy; Maxwell, D. P.

doi:10.1094/phyto-84-321

Cited by 66 publications

(33 citation statements)

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“…To date, no unequivocal, naturally occurring reassortants have been discovered in planta, although compelling evidence for such phenomena has been provided (8,17,29). Experimental reassortment has been carried out with test hosts that are susceptible to the parental (wild-type) viruses.…”

Section: Squash Leaf Curl China Virusmentioning

confidence: 99%

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Idris

Mills-Lujan

Martin

et al. 2008

J Virol

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The genome components of the Melon chlorotic leaf curl virus (MCLCuV) were cloned from symptomatic cantaloupe leaves collected in Guatemala during 2002. The MCLCuV DNA-A and DNA-B components shared their closest nucleotide identities among begomoviruses, at ϳ90 and 81%, respectively, with a papaya isolate of MCLCuV from Costa Rica. The closest relatives at the species level were other members of the Squash leaf curl virus (SLCV) clade, which is endemic in the southwestern United States and Mexico. Biolistic inoculation of cantaloupe seedlings with the MCLCuV DNA-A and -B components resulted in the development of characteristic disease symptoms, providing definitive evidence of causality. MCLCuV experimentally infected species within the Cucurbitaceae, Fabaceae, and Solanaceae. The potential for interspecific reassortment was examined for MCLCuV and its closest relatives, including the bean-restricted Bean calico mosaic virus (BCaMV), and three other cucurbit-infecting species, Cucurbit leaf crumple virus (CuLCrV), SLCV, and SMLCV. The cucurbit viruses have distinct but overlapping host ranges. All possible reassortants were established using heterologous combinations of the DNA-A or DNA-B components. Surprisingly, only certain reassortants arising from MCLCuV and BCaMV, or MCLCuV and CuLCrV, were viable in bean, even though it is a host of all of the "wild-type" (parent) viruses. The bean-restricted BCaMV was differentially assisted in systemically infecting the cucurbit test species by the components of the four cucurbit-adapted begomoviruses. In certain heterologous combinations, the BCaMV DNA-A or -B component was able to infect one or more cucurbit species. Generally, the reassortants were less virulent in the test hosts than the respective wild-type (parent) viruses, strongly implicating adaptive modulation of virulence. This is the first illustration of reassortment resulting in the host range expansion of a host-restricted begomovirus.

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Section: Squash Leaf Curl China Virusmentioning

confidence: 99%

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Idris

Mills-Lujan

Martin

et al. 2008

J Virol

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“…CTTAT TATTAC CTTAGTATTAC X(TA)TA ATATTA C CTAGTATTAC TATAGTATTAC BBTV-C1 BBTV-C2 (Yeh et al, 1994) GEMINI (Faria et al, 1994) CFDV (Rohde et al, 1990) FBNYV (AATAAG) which falls 34 nucleotides upstream from the TGA stop codon (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

“…Lazarowitz et al (1992) and Fontes et al (1994) have pointed out that the stem-loop structure is highly conserved among the components of each geminivirus and have suggested that it is necessary for DNA replication and may be part of the viral replication origin. Partial comparisons of the noncoding region of FBNYV DNA 1 with that of BBTV Yeh et al, 1994), CFDV (Rohde et at., 1990) and several geminiviruses (Faria et at., 1994) confirmed earlier findings that the potential loop structure is a highly conserved region among these viruses (Fig. 3b).…”

Section: Introductionmentioning

confidence: 99%

Sequence analysis of a faba bean necrotic yellows virus DNA component containing a putative replicase gene

Katul¹,

Maiß²,

Hj³

1995

Journal of General Virology

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with the NTP-binding motif GGEGKS. No obvious functions could be assigned to two smaller ORFs (7"4 and 9-3 kDa) occurring in the complementary oftentation. Amino acid sequence comparisons of the putative replicase of FBNYV with that of other similar ssDNA viruses yielded higher homologies to subterranean clover stunt virus than to banana bunchy top and coconut foliar decay viruses. A potential stem-loop structure and a TATA box were identified within the noncoding region. Two oligonucleotides derived from FBNYV DNA 1 were used for direct sequencing of the virion ssDNA to determine its virion polarity and for amplifying part of this component by immunocapture PCR in extracts from FBNYV-infected plants.

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“…For other begomoviruses [e.g. Dominican Republic (DR) and Guatemalan (GA) isolates of BGMV, common and severe strains of TGMV, and SqLCV isolates with broad and restricted host ranges], exchange of genomic components resulted in infectious pseudorecombinants (Lazarowitz, 1991;von Arnim & Stanley, 1992;Ingham & Lazarowitz, 1993;Faria et al, 1994) and induced symptoms resembling wild-type infections, except for BGMV-[DR] DNA-A and BGMV-[GA] DNA-B, which produced delayed and attenuated symptoms (Faria et al, 1994). In the latter case, the heterologous combinations may not interact as efficiently as the homologous combinations.…”

Section: Discussionmentioning

confidence: 99%

“…Viable pseudorecombinants have been produced in the laboratory by reassortment of the genomic components of closely related virus isolates of ACMV, squash leaf curl virus (SqLCV), tomato golden mosaic virus (TGMV) and bean golden mosaic virus (BGMV) (Stanley et al, 1985;Lazarowitz, 1991;von Arnim & Stanley, 1992;Ingham & Lazarowitz, 1993;Faria et al, 1994) or from isolates of the two species Tomato mottle virus and Bean dwarf mosaic virus (Gilbertson et al, 1993). However, in no case was pseudorecombination between viruses belonging to two different species shown to lead to a more virulent virus.…”

Section: Introductionmentioning

confidence: 99%

Supervirulent pseudorecombination and asymmetric synergism between genomic components of two distinct species of begomovirus associated with severe tomato leaf curl disease in India

Chakraborty

Ramachandran

Chattopadhyay

et al. 2008

Journal of General Virology

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Isolates of two distinct begomovirus species, the severe strain of the species Tomato leaf curl New Delhi virus (tomato leaf curl New Delhi virus-[India:New Delhi:Severe:1992]; ToLCNDV-[IN:ND:Svr:92], bipartite) and the Varanasi strain of the species Tomato leaf curl Gujarat virus (tomato leaf curl Gujarat virus-[India:Varanasi:2001]; ToLCGV-[IN:Var:01], mono/bipartite) infect tomato (Lycopersicon esculentum) and cause severe yield losses in northern India. This study investigated the infectivity properties of genomic components of these two species. Both pseudorecombinants were infectious in Nicotiana benthamiana, Nicotiana tabacum and L. esculentum. Enhanced pathogenicity was observed when DNA-A of ToLCNDV-[IN:ND:Svr:92] was trans-complemented with ToLCGV-[IN:Var:01] DNA-B, and was consistently associated with an increase in accumulation of ToLCGV-[IN:Var:01] DNA-B. Mixed infection of ToLCNDV-[IN:ND:Svr:92] and ToLCGV-[IN:Var:01] always showed extremely severe symptoms, suggesting a synergistic interaction between these two viruses. Southern blot analysis of viral DNAs from infected plants showed a significantly higher level of accumulation of both ToLCNDV components and DNA-B of ToLCGV-[IN:Var:01] with no alteration to levels of DNA-A of ToLCGV-[IN:Var:01]. Symptom development and/or higher infectivity of the supervirulent pseudorecombinants correlated with the increased levels of DNA-B accumulation. Protoplast replication assays revealed that enhanced infectivity by the pseudorecombinant occurred at the level of replication, as DNA-A of ToLCNDV-[IN:ND:Svr:92] enhanced ToLCGV-[IN:Var:01] DNA-B replication, whose accumulation was in turn increased by ToLCGV-[IN:Var:01] DNA-A. This is the first report demonstrating a virulent pseudorecombinant between two distinct species of begomoviruses that infect tomato, and is the second report on synergism between begomoviruses. The results revealed that ToLCGV-[IN:Var:01] DNA-B is capable of associating with different DNA-A components, despite having different iteron sequences.

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Bean Golden Mosaic Geminivirus Type II Isolates from the Dominican Republic and Guatemala: Nucleotide Sequences, Infectious Pseudorecombinants, and Phylogenetic Relationships

Cited by 66 publications

References 0 publications

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Melon Chlorotic Leaf Curl Virus : Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus

Sequence analysis of a faba bean necrotic yellows virus DNA component containing a putative replicase gene

Supervirulent pseudorecombination and asymmetric synergism between genomic components of two distinct species of begomovirus associated with severe tomato leaf curl disease in India

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