Basal levels and alcohol-induced changes in nociceptin/orphanin FQ, dynorphin, and enkephalin levels in C57BL/6J mice

Ploj, Karolina; Roman, Erika; Gustavsson, Lisa; Nylander, Ingrid

doi:10.1016/s0361-9230(00)00328-2

Cited by 66 publications

(47 citation statements)

References 47 publications

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“…The lowest amount of OFQ/N-IR was detected in the cerebellum and striatum. The regional distribution of OFQ/N-IR in the present study is consistent with previous observations in rat (Lindholm et al 2002), mouse (Ploj et al 2000) or human (Witta et al 2004) brain. The novelty of our study is that a single cocaine administration, as compared to saline, decreased the level of OFQ/N-IR in the midbrain and to a lesser extent in the striatum and cortical regions in rats, showing that cocaine could reduce tissue content of OFQ/N-IR, a response that we interpreted as an increase in the release or degradation of endogenous OFQ/N.…”

Section: Discussionsupporting

confidence: 93%

Alterations in the level of OFQ/N-IR in rat brain regions by cocaine

2008

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We have previously shown that administration of orphanin FQ/nociceptin (OFQ/N), the endogenous ligand of the opioid receptor-like (ORL-1) receptor, into the lateral ventricles or VTA blocked cocaine sensitization. In the present study, we determined the effect of acute and chronic cocaine treatment on the level of endogenous OFQ/N in rat brain regions. Male Sprague Dawley rats were tested for motor activity in response to saline or cocaine (20 mg/kg) injection once daily for three consecutive days. To determine the effect of single or repeated cocaine administration on the level of OFQ/N, rats were sacrificed one hour following saline or cocaine injection either on day 1 or 3, respectively. Additional groups of rats were treated similarly with saline or cocaine on days 1-3 and sacrificed or tested for locomotor sensitization on day 8. Consistent with previous studies, repeated cocaine administration induced locomotor sensitization to a challenge dose of cocaine (7.5 mg/kg) given on day 8. Measurements of tissue content of OFQ/N-IR using radioimmunoassay indicated that the rat hypothalamus and striatum respectively contained the highest and lowest level of the peptide among the brain regions tested. Acute cocaine decreased the level of OFQ-IR in the rat midbrain and to a lesser extent in the striatum. On the other hand, the level of OFQ/N was higher in rats treated with cocaine on days 1-3 and sacrificed on day 8. These findings suggest that endogenous OFQ/N may be involved in the actions of cocaine and possibly in cocaine-induced motor stimulation and locomotor sensitization.

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Section: Discussionsupporting

confidence: 93%

Alterations in the level of OFQ/N-IR in rat brain regions by cocaine

2008

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“…N/OFQ-like immunoreactivity (N/OFQ-LI) present in microdialysis fractions was measured by a specific radioimmunoassay (Ploj et al, 2000). Lyophilized samples, reconstituted in 25 l of methanol in 0.1% HCl (1:1), were mixed with 100 l of […”

Section: Recovery and Analysis Of Endogenous N/ofq (Experiments 7)mentioning

confidence: 99%

Blockade of Nociceptin/Orphanin FQ Transmission Attenuates Symptoms and Neurodegeneration Associated with Parkinson's Disease

Marti¹,

Mela²,

Fantin³

et al. 2005

J. Neurosci.

105

216

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“…eral days. The presence of the dynorphin A-containing nervous system in the amygdala is well documented in the rat (Fallon and Leslie 1986;Ma et al 2003) and mouse (Jamensky and Gianoulakis 1997;Ploj et al 2000). Interestingly, dynorphin-containing neurons in the amygdala coexist with corticotroph releasing hormone (CRH) (Marchant et al 2007), which serves as a neurotransmitter regulating diverse extra-hypophysial systems under stress (Vale et al 1981).…”

Section: Discussionmentioning

confidence: 99%

“…Its precursor, prodynorphin, produces α-and β-neoendorphin, dynorphin A and B (rimorphin), Leu-enkephalin, and a C-terminal-extended dynorphin B (leumorphin) (Day and Akil 1989;Day et al 1998). Dynorphin is widely distributed in the brain, especially in the limbic system, hypothalamus, striatum, locus coeruleus and nucleus accumbens in the rat (Fallon and Leslie 1986;Ma et al 2003) and mouse (Jamensky and Gianoulakis 1997;Ploj et al 2000). Dynorphin specifically binds the κ-opioid receptor and has a protective role for stress through the κ-receptor.…”

Section: Introductionmentioning

confidence: 99%

Occlusal Disharmony Transiently Impairs Learning and Memory in the Mouse by Increasing Dynorphin A Levels in the Amygdala

Yamada

Ono

Kubo

et al. 2013

Tohoku J. Exp. Med.

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Occlusal disharmony sometimes causes not only stiffness of neck but also psychiatric depression, suggesting that the condition of oral cavity may affect the central nervous system. Dynorphin A is an endogenous opioid peptide that specifically binds the κ-opioid receptor and has a protective role against stress. Dynorphinergic nervous system is intensely distributed in the amygdala and hippocampus that are coping areas with stress. As a model of malocclusion, we placed dental resin on the molars to increase the occlusal vertical dimension (bite-raise). After various survival times, we analyzed the amygdala and hippocampus by immunohistochemistry and immunosorbent assay (ELISA). Furthermore, the effects on learning and memory were assessed by Morris water maze test. In the amygdala, the levels of dynorphin A were increased on the 1st day after increasing the vertical dimension as indicated by immunohistochemical and ELISA assessments. The levels of dynorphin A returned to control levels on the 5th day. In the hippocampus, there were no noticeable changes in dynorphin A levels. The water maze test indicated that increasing the vertical dimension caused longer escape latency times on the 3rd day compared to those of sham-operated group. However, the bite-raised mice treated with a dynorphin antagonist, nor-binaltorphimine, showed similar escape latency times to the times of sham-operated group, even on the 3rd day. These results suggest that occlusal disharmony causes stress resulting in a transient increase of dynorphin A levels at least in the amygdala and that the increased dynorphin A levels transiently impair learning and memory.

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Basal levels and alcohol-induced changes in nociceptin/orphanin FQ, dynorphin, and enkephalin levels in C57BL/6J mice

Cited by 66 publications

References 47 publications

Alterations in the level of OFQ/N-IR in rat brain regions by cocaine

Alterations in the level of OFQ/N-IR in rat brain regions by cocaine

Blockade of Nociceptin/Orphanin FQ Transmission Attenuates Symptoms and Neurodegeneration Associated with Parkinson's Disease

Occlusal Disharmony Transiently Impairs Learning and Memory in the Mouse by Increasing Dynorphin A Levels in the Amygdala

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