Barley FASCIATED EAR genes determine inflorescence meristem size and yield traits

Wang, Chengyu; Yang, Xiujuan; Zhang, Yueya; Shen, Chaoqun; Jin, Shi; Xia, Chongjing; Fang, Taohong; Tu, Qiang; Li, Ling; Zhou, Xinli; Zhang, Dabing; Li, Gang

doi:10.1016/j.cj.2022.10.001

Cited by 6 publications

(1 citation statement)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Poaceae family is one of the largest angiosperm families and has evolved complex inflorescence morphologies, such as branches and spikelets (Zhang and Yuan, 2014). In maize, the inflorescence structures initially derive from inflorescence meristem (IM) which is responsible for initiating lateral primordia (Wang et al, 2022). IM is transferred from shoot apical meristem (SAM), which initiates leaf development during the reproductive transition.…”

Section: Introductionmentioning

confidence: 99%

Genetic mapping and functional analysis of a classical tassel branch number mutant Tp2 in maize

Wang

Wei

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Tassel branch number is a key trait that contributes greatly to grain yield in maize (Zea mays). We obtained a classical mutant from maize genetics cooperation stock center, Teopod2 (Tp2), which exhibits severely decreased tassel branch. We conducted a comprehensive study, including phenotypic investigation, genetic mapping, transcriptome analysis, overexpression and CRISPR knock-out, and tsCUT&Tag of Tp2 gene for the molecular dissection of Tp2 mutant. Phenotypic investigation showed that it is a pleiotropic dominant mutant, which is mapped to an interval of approximately 139-kb on Chromosome 10 harboring two genes Zm00001d025786 and zma-miR156h. Transcriptome analysis showed that the relative expression level of zma-miR156h was significantly increased in mutants. Meanwhile, overexpression of zma-miR156h and knockout materials of ZmSBP13 exhibited significantly decreased tassel branch number, a similar phenotype with Tp2 mutant, suggesting that zma-miR156h is the causal gene of Tp2 and targets ZmSBP13 gene. Besides, the potential downstream genes of ZmSBP13 were uncovered and showed that it may target multiple proteins to regulate inflorescence structure. Overall, we characterized and cloned Tp2 mutant, and proposed a zma-miR156h-ZmSBP13 model functioning in regulating tassel branch development in maize, which is an essential measure to satisfy the increasing demands of cereals.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic mapping and functional analysis of a classical tassel branch number mutant Tp2 in maize

Wang

Wei

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

Precise Gene Editing of Cereals Using CRISPR/Cas Technology

Pouramini,

Hensel

2023

A Roadmap for Plant Genome Editing

View full text Add to dashboard Cite

Targeted mutagenesis using CRISPR/Cas technology has become routine in elucidating biological processes or their application in breeding and agriculture. This means that the change to be achieved can be accurately predicted. However, knockout of a gene function is not always desirable, as reducing activity or affecting a protein domain can influence its properties and, thus, the phenotype. This chapter will therefore focus on precise genome modification in temperate cereals. The methods used, including some representative examples, are summarised here.

show abstract

CRISPR/Cas9-mediated genome editing techniques and new breeding strategies in cereals – current status, improvements, and perspectives

Ahmar,

Hensel,

Gruszka

2023

Biotechnology Advances

View full text Add to dashboard Cite

Barley FASCIATED EAR genes determine inflorescence meristem size and yield traits

Cited by 6 publications

References 63 publications

Genetic mapping and functional analysis of a classical tassel branch number mutant Tp2 in maize

Genetic mapping and functional analysis of a classical tassel branch number mutant Tp2 in maize

Precise Gene Editing of Cereals Using CRISPR/Cas Technology

CRISPR/Cas9-mediated genome editing techniques and new breeding strategies in cereals – current status, improvements, and perspectives

Contact Info

Product

Resources

About