Bark depolymerization during submerged fermentation using monofloral honey, a natural mediator substitute, and integration between laccases vs. bark biopolymers, characterized by Py-GC-MS

Ferhan, Muhammad; Yan, Ning; Sain, Mohini

doi:10.1039/c4ra13841d

Cited by 2 publications

(2 citation statements)

References 63 publications

(54 reference statements)

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“…The enzyme kinetics parameters K m and k cat of native GOx, GOx@cPAAm, and GOx@Fla-c-PAAm were calculated by Lineweaver–Burk plots defined by the following equation where K m is Michaelis constant. V max is the maximum reaction rate of the enzyme.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of Activity-Reporting Glucose Oxidase Nanocapsules with Oxygen-Independent Fluorescence Variation

Chen

Huang

Jiang

et al. 2018

ACS Appl. Mater. Interfaces

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Glucose oxidase (GOx) has seen large-scale technological applications, and the determinations of its activity that is directly related to the enzymatic functions are extremely important. However, conventional methods to analyze the enzymatic activity involving high oxygen dependency and indirect redox reactions are usually tedious and restricted in complicated environments. For analyzing enzymatic activity by direct detection of the electron signals from the active centers, mediators are often used for facilitating the electron transfer. Differing from common methods of preparing electron mediators-contained GOx composites, a strategy aiming at remolding of the enzyme itself has been proposed in this work. Cofactor-like molecule 2'-diallyamino-ethyl flavin (DAA-flavin) derived from riboflavin is synthesized and incorporated as cross-linker into the polyacrylamide (PAAm) network around GOx surface by in situ polymerization to obtain enzyme nanocapsules termed as GOx@Fla-c-PAAm. The peripheral polymer shell confines the orientation of GOx and prevents it from denaturing, whereas incorporated DAA-flavin can replace the oxygen as an alternative electron acceptor to interact with the active centers of GOx in the presence of the substrate, thus giving the nanocapsules oxygen-independent characteristics. The introduced unlimited cofactor-like molecules endow the nanocapsules redox-related fluorescence, and the intensity variation is closely correlated with the enzymatic activity. There is a high goodness of fitting ( R ∼ 0.990) between the slope of linear fluorescence-time plots and enzymatic activity, thereby making the nanocapsules a reliable activity-reporting enzymatic nanosystem with oxygen-independent fluorescence variation for further extended potential application in biofuel cells and biosensors.

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Section: Resultsmentioning

confidence: 99%

Fabrication of Activity-Reporting Glucose Oxidase Nanocapsules with Oxygen-Independent Fluorescence Variation

Chen

Huang

Jiang

et al. 2018

ACS Appl. Mater. Interfaces

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“…The low reactivity of lignin hinders the direct utilization of lignin, so the modification of lignin has been developed as an alternative strategy to improve the lignin application [100,101]. The most widely used methods include demethylation [102,103], methylolation [104,105], phenolation [106,107], oxidative degradation and hydrothermal depolymerization/liquefaction [108,109]. Fungi and bacteria have been used for decades to carry out the demethylation of lignin, with advantages of less investments and shorter processing times.…”

Section: Demethylationmentioning

confidence: 99%

Lignin Biodegradation and Its Valorization

et al. 2022

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Lignin, a rigid polymer composed of phenolic subunits with high molecular weight and complex structure, ranks behind only cellulose in the contribution to the biomass of plants. Therefore, lignin can be used as a new environmentally friendly resource for the industrial production of a variety of polymers, dyes and adhesives. Since laccase was found to be able to degrade lignin, increasing attention had been paid to the valorization of lignin. Research has mainly focused on the identification of lignin-degrading enzymes, which play a key role in lignin biodegradation, and the potential application of lignin degradation products. In this review, we describe the source, catalytic specificity and enzyme reaction mechanism of the four classes of the lignin-degrading enzymes so far discovered. In addition, the major pathways of lignin biodegradation and the applications of the degradative products are also discussed. Lignin-degrading bacteria or enzymes can be used in combination with chemical pretreatment for the production of value-added chemicals from lignin, providing a promising strategy for lignin valorization.

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Bark depolymerization during submerged fermentation using monofloral honey, a natural mediator substitute, and integration between laccases vs. bark biopolymers, characterized by Py-GC-MS

Abstract: This is a new green biochemical approach to edify that how we can replace synthetic, toxic and costly mediators into natural mediator using monofloral honey which accelerates oxidation mechanism with combination of fungal laccases.

Cited by 2 publications

References 63 publications

Fabrication of Activity-Reporting Glucose Oxidase Nanocapsules with Oxygen-Independent Fluorescence Variation

Fabrication of Activity-Reporting Glucose Oxidase Nanocapsules with Oxygen-Independent Fluorescence Variation

Lignin Biodegradation and Its Valorization

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