Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.03.051 View full text
Fabrice Gritti, Georges Guiochon

Abstract: The sources of band broadening in fast gradient elution chromatography include the contributions of the tubings placed upstream the column, the isocratic migration of the sample that takes place in the column until the moment when the front of the gradient catches up with it, dispersion and mass transfer resistance during band migration under gradient elution to the column outlet, and dispersion in the tubings downstream the column to the detector. The measurements of all these contributions is meticulously de…

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