Baltetin: a new C-type lectin-like isolated from Bothrops alternatus snake venom which act as a platelet aggregation inhibiting

Pereira, Déborah Fernanda da Cunha; Ribeiro, Mariana Santos Matias; Simamoto, Bruna Barbosa de Sousa; Dias, Edigar Henrique Vaz; Costa, Júnia de Oliveira; Santos-Filho, Norival A.; Bordon, Karla de Castro Figueiredo; Arantes, Eliane Candiani; Dantas, Noélio O.; Silva, Anielle Christine Almeida; Oliveira, Fábio de; Mamede, Carla Cristine Neves

doi:10.1016/j.jchromb.2021.122695

Cited by 3 publications

(1 citation statement)

References 63 publications

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“…80 Baltetin and BmLec are snaclecs capable of inhibiting and inducing platelet aggregation, respectively. 81,82 BmooAi is a protein that inhibits platelet aggregation, but its class has not yet been determined. It showed a very specific inhibitory effect on platelet aggregation induced by collagen, ADP, or epinephrine in human platelet-rich plasma in a dose-dependent manner, whereas it had little or no effect on platelet aggregation induced by ristocetin.…”

Section: Other Classesmentioning

confidence: 99%

Bothrops Moojeni Snake Venom: A Source of Potential Therapeutic Agents Against Hemostatic Disorders

Silva,

Dias,

Costa

et al. 2024

International Journal of Cardiovascular Sciences

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Elements of hemostasis: a blood coagulation cascade is initiated by release of chemical messengers such as TF and vWF injured tissue, activating the extrinsic coagulation pathway. Platelets meet tissue and release active compounds such as ADP, TXA2, ATP (Adenosine Triphosphate), GTP (Guanosine Triphosphate), and 5HT (Serotonin) that aggregate platelet amplification. Then, there is the conversion of prothrombin to thrombin by intrinsic and extrinsic factors of the coagulation cascade. Thrombin, together with fibrinogen and Ca2+ (calcium ions), form fibrin and activate factors V, VII, VIII, XII, and XIII for fibrin clot formation and stabilization. It occurs the clot fibrinolysis and plasmin-mediated destruction of coagulation factors, local from plasminogen and its activators. The dashed and solid arrows indicate, respectively, the activation and inhibition of various stages of the blood clotting process. TF: tissue factor; vWF: von Willebrand factor.Central Illustration: Bothrops moojeni proteins acting on the different components of hemostasis.

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Section: Other Classesmentioning

confidence: 99%

Bothrops Moojeni Snake Venom: A Source of Potential Therapeutic Agents Against Hemostatic Disorders

Silva,

Dias,

Costa

et al. 2024

International Journal of Cardiovascular Sciences

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Crosstalk of Inflammation and Coagulation in Bothrops Snakebite Envenoming: Endogenous Signaling Pathways and Pathophysiology

Cavalcante

Almeida

Santos-Filho

et al. 2023

IJMS

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Snakebite envenoming represents a major health problem in tropical and subtropical countries. Considering the elevated number of accidents and high morbidity and mortality rates, the World Health Organization reclassified this disease to category A of neglected diseases. In Latin America, Bothrops genus snakes are mainly responsible for snakebites in humans, whose pathophysiology is characterized by local and systemic inflammatory and degradative processes, triggering prothrombotic and hemorrhagic events, which lead to various complications, organ damage, tissue loss, amputations, and death. The activation of the multicellular blood system, hemostatic alterations, and activation of the inflammatory response are all well-documented in Bothrops envenomings. However, the interface between inflammation and coagulation is still a neglected issue in the toxinology field. Thromboinflammatory pathways can play a significant role in some of the major complications of snakebite envenoming, such as stroke, venous thromboembolism, and acute kidney injury. In addition to exacerbating inflammation and cell interactions that trigger vaso-occlusion, ischemia–reperfusion processes, and, eventually, organic damage and necrosis. In this review, we discuss the role of inflammatory pathways in modulating coagulation and inducing platelet and leukocyte activation, as well as the inflammatory production mediators and induction of innate immune responses, among other mechanisms that are altered by Bothrops venoms.

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Separation and Analytical Techniques Used in Snake Venomics: A Review Article

et al. 2022

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The deleterious consequences of snake envenomation are due to the extreme protein complexity of snake venoms. Therefore, the identification of their components is crucial for understanding the clinical manifestations of envenomation pathophysiology and for the development of effective antivenoms. In addition, snake venoms are considered as libraries of bioactive molecules that can be used to develop innovative drugs. Numerous separation and analytical techniques are combined to study snake venom composition including chromatographic techniques such as size exclusion and RP-HPLC and electrophoretic techniques. Herein, we present in detail these existing techniques and their applications in snake venom research. In the first part, we discuss the different possible technical combinations that could be used to isolate and purify SV proteins using what is known as bioassay-guided fractionation. In the second part, we describe four different proteomic strategies that could be applied for venomics studies to evaluate whole venom composition, including the mostly used technique: RP-HPLC. Eventually, we show that to date, there is no standard technique used for the separation of all snake venoms. Thus, different combinations might be developed, taking into consideration the main objective of the study, the available resources, and the properties of the target molecules to be isolated.

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Baltetin: a new C-type lectin-like isolated from Bothrops alternatus snake venom which act as a platelet aggregation inhibiting

Cited by 3 publications

References 63 publications

Bothrops Moojeni Snake Venom: A Source of Potential Therapeutic Agents Against Hemostatic Disorders

Bothrops Moojeni Snake Venom: A Source of Potential Therapeutic Agents Against Hemostatic Disorders

Crosstalk of Inflammation and Coagulation in Bothrops Snakebite Envenoming: Endogenous Signaling Pathways and Pathophysiology

Separation and Analytical Techniques Used in Snake Venomics: A Review Article

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