Baicalin inhibits inflammation and attenuates myocardial ischaemic injury by aryl hydrocarbon receptor

Xue, Yiqiang; Shui, Xiaorong; Su, Weiqing; He, Yuan; Lu, Xin-Jiang; Yan, Guosen; Huang, Shian; Lei, Wei; Chen, Can

doi:10.1111/jphp.12484

Cited by 26 publications

(16 citation statements)

References 50 publications

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“…Therefore, cytokines expression and neutrophil infiltration are central mediators of inflammation. On the other hand, previous evidence showed that baicalin inhibited inflammatory responses via down-regulation of cytokines in an animal model with myocardial ischemic injury [26]. Another report also showed that baicalin suppressed the production of cytokines and leukocyte migration in in vitro and in vivo studies [27].…”

Section: Discussionmentioning

confidence: 99%

Baicalin Attenuates IL-17-Mediated Acetaminophen-Induced Liver Injury in a Mouse Model

et al. 2016

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BackgroundIL-17 has been shown to be involved in liver inflammatory disorders in both mice and humans. Baicalin (BA), a major compound extracted from traditional herb medicine (Scutellariae radix), has potent hepatoprotective properties. Previous study showed that BA inhibits IL-17-mediated lymphocyte adhesion and downregulates joint inflammation. The aim of this study is to investigate the role of IL-17 in the hepatoprotective effects of BA in an acetaminophen (APAP)-induced liver injury mouse model.MethodsEight weeks male C57BL/6 (B6) mice were used for this study. Mice received intraperitoneal hepatotoxic injection of APAP (300 mg/kg) and after 30 min of injection, the mice were treated with BA at a concentration of 30 mg/kg. After 16 h of treatment, mice were killed. Blood samples and liver tissues were harvested for analysis of liver injury parameters.ResultsAPAP overdose significantly increased the serum alanine transferase (ALT) levels, hepatic activities of myeloperoxidase (MPO), expression of cytokines (TNF-α, IL-6, and IL-17), and malondialdehyde (MDA) activity when compared with the control animals. BA treatment after APAP administration significantly attenuated the elevation of these parameters in APAP-induced liver injury mice. Furthermore, BA treatment could also decrease hepatic IL-17-producing γδT cells recruitment, which was induced after APAP overdose.ConclusionOur data suggested that baicalin treatment could effectively decrease APAP-induced liver injury in part through attenuation of hepatic IL-17 expression. These results indicate that baicalin is a potential hepatoprotective agent.

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Section: Discussionmentioning

confidence: 99%

Baicalin Attenuates IL-17-Mediated Acetaminophen-Induced Liver Injury in a Mouse Model

et al. 2016

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“…For example, Baicalin was reported to ameliorate isoproterenol‐triggered acute myocardial infarction by suppressing inducible nitric oxide synthase, oxidative stress, inflammation, and P38MAPK pathway in rat (Sun, Wu, Song, & Li, ). Besides that, Xue et al reported that Baicalin attenuated myocardial ischemic damage by suppressing the inflammatory response via devitalizing the aryl hydrocarbon receptor (Xue et al, ). However, the effect of Baicalin on myocarditis has not been studied thoroughly.…”

Section: Introductionmentioning

confidence: 99%

Retracted: Baicalin relieves lipopolysaccharide‐evoked inflammatory injury through regulation of miR‐21 in H9c2 cells

Liu

Wang

Zhao

2020

Phytotherapy Research

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Myocarditis is a common heart disease which lacks effective treatment till now.Baicalin possesses plenty of activities, including anti-inflammation. In this investigation, we attempted to investigate the influences of Baicalin on Lipopolysaccharide (LPS)-evoked H9c2 cells.Cells viability, apoptosis, and expressions of apoptosisassociated proteins were, respectively, measured utilizing CCK-8 assay, flow cytometry and western blot. The levels of IL-6 and TNF-α were detected through enzyme-linked immunosorbent assay, western blot and qRT-PCR. miR-21 expression was detected through qRT-PCR and was silenced using cell transfection. The expressions of NF-κB and PDCD4/JNK pathways related proteins were measured through western blot. We found that LPS stimulation induced cell apoptosis and upregulation of IL-6 and TNF-α. Baicalin treatment effectively suppressed LPS-induced inflammation and apoptosis. The NF-κB and PDCD4/JNK pathways were blocked by Baicalin. Additionally, the enhanced expression of miR-21 triggered by LPS was further elevated by Baicalin. Further study revealed that the inhibiting effects of Baicalin on LPS-evoked injury were largely attenuated by knockdown of miR-21. Moreover, the associated NF-κB and JNK pathways, which were suppressed by Baicalin treatment, were then activated by knockdown of miR-21. Our present study revealed that Baicalin alleviated LPS-evoked inflammatory injury via suppressing the NF-κB and PDCD4/JNK pathways through regulating miR-21 expression. K E Y W O R D S apoptosis, Baicalin, inflammatory injury, MiR-21, myocarditis

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“…baicalensis is mainly distributed in East Asia and its dry roots were prevalently used to treat inflammatory and bacterial diseases as old-line China traditional medicine (Yamamoto, 1991;Huang et al, 2012;Xue et al, 2015). In present study, the bioinformatic analyses of 4cl family from S. baicalensis were completed, which would pave for further studies of physichemical properties of 4CL protein family and its related molecular mechanism of flavonoid biosynthesis.…”

Section: Fig 1 Flavonoid Biosynthetic Pathway In Scutellaria Cellsmentioning

confidence: 93%

<i>In silico</i> Analysis of 4CL Family in <i>Scutellaria baicalensis</i> through Biocomputational Tools and Servers

Li¹,

Lan²,

Shui³

et al. 2017

American Journal of Biochemistry and Biotechnology

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The gene sequences of 4-coumarate:coenzyme A ligase (4CL) family of Scutellaria baicalensis came from the GenBank Database. With help of some bioinformatics tools such as Vector NTI Suite 8, ProtParam and SWISS-MODEL and so on, a series of biological information of their nucleic acid sequences and amino acid sequences were predicted and analyzed and the results were revealed as following: Two 4cl member genes share high similarity in structure and properties on level of nucleic acid and amino acid molecular. 4CLs are hydrophic proteins without any transmembrane topological structure and some crucial motifs were found. The secondary structures of Sb4CLs are mainly composed of random coil and α-helix and the models of their tertiary structures were built. In silico analysis of Sv4CL was finished, which would pave for further studies of physichemical properties of 4CL family and its related molecular mechanism of flavonoid metabolic regulation.

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Baicalin inhibits inflammation and attenuates myocardial ischaemic injury by aryl hydrocarbon receptor

Cited by 26 publications

References 50 publications

Baicalin Attenuates IL-17-Mediated Acetaminophen-Induced Liver Injury in a Mouse Model

Baicalin Attenuates IL-17-Mediated Acetaminophen-Induced Liver Injury in a Mouse Model

Retracted: Baicalin relieves lipopolysaccharide‐evoked inflammatory injury through regulation of miR‐21 in H9c2 cells

<i>In silico</i> Analysis of 4CL Family in <i>Scutellaria baicalensis</i> through Biocomputational Tools and Servers

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