Baicalein protects HT22 murine hippocampal neuronal cells against endoplasmic reticulum stress-induced apoptosis through inhibition of reactive oxygen species production and CHOP induction

Choi, Ji Hyun; Choi, Ae Jin; Yoon, Hana; Choe, Wonchae; Yoon, Kyung‐Sik; Ha, Joohun; Yeo, Eui‐Ju; Kang, Insug

doi:10.3858/emm.2010.42.12.084

Cited by 110 publications

(75 citation statements)

References 41 publications

(71 reference statements)

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“…However, glutamate-induced cytotoxicity is associated not only with the excitotoxic pathway, but also with ROS-driven oxidative stress (15,63). We examined whether glutamate-induced HT22 injuries are associated with reduced expression of Prdx6 and increased oxidative load.…”

Section: Glutamate Induced Cell Death and Increased Ros Production Wimentioning

confidence: 99%

Delivery of a protein transduction domain-mediated Prdx6 protein ameliorates oxidative stress-induced injury in human and mouse neuronal cells

Singh

Chhunchha

Fatma

et al. 2016

American Journal of Physiology-Cell Physiology

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Section: Glutamate Induced Cell Death and Increased Ros Production Wimentioning

confidence: 99%

Delivery of a protein transduction domain-mediated Prdx6 protein ameliorates oxidative stress-induced injury in human and mouse neuronal cells

Singh

Chhunchha

Fatma

et al. 2016

American Journal of Physiology-Cell Physiology

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“…Baicalein (BAI,5,6, is obtained from Traditional Chinese Medicine -Scutellaria baicalensis with diverse interesting bioactivities such as anti-inflammation (Lee et al, 2011), attenuating oxidative stress (Choi et al, 2010;Stavniichuk et al, 2011) and anticancer (Wang et al, 2010;Takahashi et al, 2011). In China, combined with other herbs, BAI has been widely used in the treatment of inflammation, fever, cough, dysentery, jaundice, hepatitis and hypertension for many years (Zhang et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Long-circulating nanoliposomes (LCNs) sustained delivery of baicalein (BAI) with desired oral bioavailabilityin vivo

Liang¹,

Liu³

et al. 2013

Drug Delivery

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Long-circulating nanoliposomes (LCNs) containing Baicalein (BAI) were prepared by diethyl ether injection method in this paper. Using soybean phosphatidylcholine (SPC) and cholesterol (CHOL) as the carrier ingredients, BAI-loaded LCNs were formulated in optimized condition with encapsulation efficiency (EE) of 41.5 AE 4.77%. The average diameter of BAI-loaded LCNs was $709 nm determined by dynamic light scattering (PDI 0.43). Drug storage stability study showed that BAI-loaded LCNs were highly stable in phosphate-buffered saline (PBS, pH 7.4) at 4 C. Additionally, the pharmacokinetic behaviors of BAI-loaded LCNs were studied using Kunming mice as experimental animals. Parallelly, free BAI solution was studied as control. As expected, it is found that LCNs-encapsulated BAI yields greater BAI oral bioavailability with 4.52 times of free BAI. These results suggest the potential applications of LCNs for the delivery of BAI.

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“…For this study, cells were trypsinized and cell pellets were re-suspended in PBS and incubated with 10 µM JC-1 for 20 min at 37˚C. Cells were subsequently washed once with cold PBS, suspended, and analyzed using a flow cytometer (19).…”

Section: Mitochondrial Membrane Potential (Mmp δψM) Assaymentioning

confidence: 99%

Bufalin induces apoptosis through activation of both the intrinsic and extrinsic pathways in human bladder cancer cells

Choi

2011

Oncol Rep

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Abstract. Bufalin, a major digoxin-like immunoreactive component of the Chinese medicine Chansu, is prepared from toad venom. This compound has been shown to exert a potential for anticancer activity against various human cancer cell lines in vitro. However, the detailed molecular mechanisms of its induction of apoptosis are still unclear. In this study, we investigated the apoptosis-inducing effect of bufalin in T24 human bladder cancer cells. Our data revealed that bufalin treatment resulted in a concentration-response growth inhibition of T24 cells by inducing cell cycle arrest at the G2/M phase and apoptosis, as evidenced by formation of apoptotic bodies, chromatin condensation and accumulation of cells in the sub-G1 phase. Apoptosis induction of T24 cells by bufalin showed correlation with proteolytic activation of caspase-3, -8 and -9, and concomitant degradation of poly (ADP-ribose) polymerases, and collapse of the mitochondria membrane potential. In addition, bufalin treatment resulted in an increase of the Bax/Bcl-2 (or Bcl-xL) ratio and caused down-regulation of inhibitor of apoptosis protein (IAP) family members. The increase in apoptosis by bufalin treatment was also associated with up-regulation of death receptor-related factors. Our data indicate that the growth inhibitory effects of bufalin occur through blockade of the G2/M phase, and that these cancer cells do not enter cell cycle progression and die through apoptosis via both intrinsic and extrinsic pathways.

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Baicalein protects HT22 murine hippocampal neuronal cells against endoplasmic reticulum stress-induced apoptosis through inhibition of reactive oxygen species production and CHOP induction

Cited by 110 publications

References 41 publications

Delivery of a protein transduction domain-mediated Prdx6 protein ameliorates oxidative stress-induced injury in human and mouse neuronal cells

Delivery of a protein transduction domain-mediated Prdx6 protein ameliorates oxidative stress-induced injury in human and mouse neuronal cells

Long-circulating nanoliposomes (LCNs) sustained delivery of baicalein (BAI) with desired oral bioavailabilityin vivo

Bufalin induces apoptosis through activation of both the intrinsic and extrinsic pathways in human bladder cancer cells

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