Baicalein attenuates inflammatory responses by suppressing TLR4 mediated NF-κB and MAPK signaling pathways in LPS-induced mastitis in mice

He, Xuexiu; Wei, Zhengkai; Zhou, Ershun; Chen, Libin; Kou, Jinhua; Wang, Jingjing; Yang, Zhengtao

doi:10.1016/j.intimp.2015.07.012

Cited by 111 publications

(72 citation statements)

References 30 publications

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“…The most abundant activated form of NF-κB is a heterodimer of p50 and p65, çontaining transcriptional activation domains necessary for gene induction (28). In resting cells, the NF-κB heterodimer is held in the cytosol through interaction with IκB inhibitory proteins.…”

Section: Discussionmentioning

confidence: 99%

Wogonin protects human retinal pigment epithelium cells from LPS-induced barrier dysfunction and inflammatory responses by regulating the TLR4/NF-κB signaling pathway

Chen

Guo

2017

Molecular Medicine Reports

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Inflammation in the retinal pigment epithelium is an important contributor to the pathogenesis of age-related macular degeneration. Wogonin is a flavonoid isolated from the root of Scutellaria baicalensis and has multiple pharmacological effects, including anti‑inflammatory effects. The present study sought to determine if the pharmacological effects of wogonin were relevant to the treatment of AMD. ARPE‑19 cells were pre‑conditioned with different concentrations of wogonin (0‑50 µM) prior to induction of inflammation with LPS (2 µg/ml). Transepithelial electrical resistance analysis demonstrated that 24 h treatment with 10 and 50 µM wogonin ameliorated LPS‑induced changes. Reverse transcription-quantitative polymerase chain reaction (RT‑qPCR) and immunoﬂuorescence analyses revealed that wogonin restrained LPS-induced tight junction proteins, claudin‑1 and ZO‑1. LPS‑induced upregulation of inflammatory mediators in ARPE‑19 cells, including IL‑1β, IL‑6, IL‑8, cyclooxygenase‑2 (COX‑2), inducible nitric oxide synthase (iNOS) and TNF‑α was reduced after pre-treatment with wogonin. In addition, RT‑qPCR and western blotting demonstrated that wogonin inhibited the expression of TLR4 in LPS‑stimulated ARPE‑19 cells. This is a novel mechanism indicating that pre‑treatment with wogonin could attenuate the TLR4/NF‑κB‑mediated inflammatory response in LPS‑stimulated ARPE‑19 cells, and thus could be a potential therapy for the treatment of AMD.

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Section: Discussionmentioning

confidence: 99%

Wogonin protects human retinal pigment epithelium cells from LPS-induced barrier dysfunction and inflammatory responses by regulating the TLR4/NF-κB signaling pathway

Chen

Guo

2017

Molecular Medicine Reports

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“…S. baicalensis contains various components, and more than 60 flavonoids have been identified from different sources of S. baicalensis [3031]. Many reports show that baicalein and wogonin are the principal active components of S. baicalensis and have potent anti-cancer and anti-inflammatory effects [18192021222331]. Here, compared with their anti-inflammatory effects, we demonstrated the anti-allergic and anti-anaphylactic effects of these flavonoids using OVA-induced asthma and compound 48/80-induced systemic anaphylaxis mice models and studied with mast cell-mediated responses in vitro .…”

Section: Discussionmentioning

confidence: 99%

“…Recently, investigations have shown that S. baicalensis has beneficial properties such as anti-oxidative effect [16], and inhibits anti-dinitrophenyl IgE-medicated anaphylactic reactions and compound 48/80-induced histamine release and calcium uptake into rat peritoneal mast cells (RPMC) [1517]. The flavonoids such as baicalein and wogonin isolated from S. baicalensis have also various biological activities such as anti-oxidative, anti-inflammatory and anti-allergic effects [181920212223]. However, despite studies on anti-allergic effects of S. baicalensis have progressed extensively, little is known about the detailed effects of baicalein, wogonin, and S. baicalensis ethanol extract on ovalbumin (OVA)-induced Th2 cytokine-dependent murine asthma model and compound 48/80-induced systemic anaphylaxis in vivo and compound 48/80-induced mast cell activation in vitro .…”

Section: Introductionmentioning

confidence: 99%

Baicalein, wogonin, andScutellaria baicalensisethanol extract alleviate ovalbumin-induced allergic airway inflammation and mast cell-mediated anaphylactic shock by regulation of Th1/Th2 imbalance and histamine release

et al. 2017

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Asthma is characterized by chronic inflammation, goblet cell hyperplasia, the aberrant production of the Th2 cytokines, and eosinophil infiltration into the lungs. In this study, we examined the effects of baicalein, wogonin, and Scutellaria baicalensis ethanol extract on ovalbumin (OVA)-induced asthma by evaluating Th1/Th2 cytokine levels, histopathologic analysis, and compound 48/80-induced systemic anaphylaxis and mast cell activation, focusing on the histamine release from rat peritoneal mast cells. Baicalein, wogonin, and S. baicalensis ethanol extract also decreased the number of inflammatory cells especially eosinophils and downregulated peribronchial and perivascular inflammation in the lungs of mice challenged by OVA. Baicalein, wogonin, and S. baicalensis ethanol extract significantly reduced the levels of tumor necrosis factor α, interleukin (IL)-1β, IL-4, IL-5 and the production of OVA-specific IgE and IgG1, and upregulated the level of interferon-γ and OVA-specific IgG2a. In addition, oral administration of baicalein, wogonin, and S. baicalensis ethanol extract inhibited compound 48/80-induced systemic anaphylaxis and plasma histamine release in mice. Moreover, baicalein, wogonin, and S. baicalensis ethanol extract suppressed compound 48/80-induced mast cell degranulation and histamine release from rat peritoneal mast cells. Conclusively, baicalein and wogonin as major flavonoids of S. baicalensis may have therapeutic potential for allergic asthma through modulation of Th1/Th2 cytokine imbalance and histamine release from mast cells.

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“…The chemical structure and properties of baicalein are shown in Figure 1. Emerging evidence has demonstrated that baicalein exerts multiple pharmacological effects including anti-inflammatory [17], antioxidant [18], and antiviral [19] properties, as well as protection against cardiovascular illness [20]. In the past decade, there has been great progress in exploring the target mechanisms and signaling pathways of baicalein’s anti-cancer potential.…”

Section: The Property and Antitumor Effect Of Baicaleinmentioning

confidence: 99%

The Fascinating Effects of Baicalein on Cancer: A Review

Liu

Dong

Gao

et al. 2016

IJMS

189

133

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Cancer is one of the leading causes of death worldwide and a major global health problem. In recent decades, the rates of both mortality and morbidity of cancer have rapidly increased for a variety of reasons. Despite treatment options, there are serious side effects associated with chemotherapy drugs and multiple forms of drug resistance that significantly reduce their effects. There is an accumulating amount of evidence on the pharmacological activities of baicalein (e.g., anti-inflammatory, antioxidant, antiviral, and antitumor effects). Furthermore, there has been great progress in elucidating the target mechanisms and signaling pathways of baicalein’s anti-cancer potential. The anti-tumor functions of baicalein are mainly due to its capacities to inhibit complexes of cyclins to regulate the cell cycle, to scavenge oxidative radicals, to attenuate mitogen activated protein kinase (MAPK), protein kinase B (Akt) or mammalian target of rapamycin (mTOR) activities, to induce apoptosis by activating caspase-9/-3 and to inhibit tumorinvasion and metastasis by reducing the expression of matrix metalloproteinase-2/-9 (MMP-2/-9). In this review, we focused on the relevant biological mechanisms of baicalein involved in inhibiting various cancers, such as bladder cancer, breast cancer, and ovarian cancer. Moreover, we also summarized the specific mechanisms by which baicalein inhibited the growth of various tumors in vivo. Taken together, baicalein may be developed as a potential, novel anticancer drug to treat tumors.

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Baicalein attenuates inflammatory responses by suppressing TLR4 mediated NF-κB and MAPK signaling pathways in LPS-induced mastitis in mice

Cited by 111 publications

References 30 publications

Wogonin protects human retinal pigment epithelium cells from LPS-induced barrier dysfunction and inflammatory responses by regulating the TLR4/NF-κB signaling pathway

Wogonin protects human retinal pigment epithelium cells from LPS-induced barrier dysfunction and inflammatory responses by regulating the TLR4/NF-κB signaling pathway

Baicalein, wogonin, andScutellaria baicalensisethanol extract alleviate ovalbumin-induced allergic airway inflammation and mast cell-mediated anaphylactic shock by regulation of Th1/Th2 imbalance and histamine release

The Fascinating Effects of Baicalein on Cancer: A Review

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