Bacteriophage Display of Combinatorial Antibody Libraries

Abstract

Bacteriophage display of combinatorial antibody libraries is one means by which monoclonal antibodies of a desired specificity can be selected without the use of conventional hybridoma technology. Phage display is the expression of proteins and peptides on the surface of a bacteriophage particle, and a combinatorial antibody library is a collection of rearranged variable‐region gene segments that have been randomly combined through the use of recombinant molecular biology techniques to yield a large ‘library’ of antibodies.