Bacteriological study of post-operative wound infections with special reference to MRSA and ESBL in a tertiary care hospital

Abstract

Background: Surgical site infections are known to be one of the most common causes of nosocomial infections worldwide and raises an important public health concern.Methods: A prospective observational study was conducted across 12 months (July 2018-June 2019) in a tertiary care hospital. The present study includes 50 pus samples from clinically suspected cases of post-operative wound infections from various surgical wards which were inoculated in Department of Microbiology. The phenotypic identification of different bacterial isolates especially MRSA and ESBL producers along with their antimicrobial susceptibility testing was interpreted according to CLSI guidelines.Results: Out of 50 samples, 44 showed positive culture. The infections were more common in emergency situations, age group of 20-30 years and in females. The most common bacterial isolates were Staphylococcus aureus (43.18%) followed by E. coli (22.72%), Klebsiella (15.9%), CONS (13.63%) and Pseudomonas (4.54%). Out of 19 Staphylococcus aureus 9 were MRSA and these gram-positive bacteria were highly sensitive to Linejolid and Clindamycin. In gram negative group 22.72% of E. coli and 15.9% of Klebsiella were ESBL producers who were susceptible mainly to Aztreonam, Linezolid or Cefoxitine.Conclusions: Post-operative wound or surgical site infections is the most important factor responsible for significant morbidity, mortality, prolonged hospitalization and additional cost to treatment in surgical patients. Marked resistance of isolates to commonly used antibiotics indicates the need of judicious use of these drugs to prevent the emergence of multi-drug resistant strains.

A Study on Surveillance of Surgical Site Infection in Surgical Ward of A Tertiary Care Hospital

“…On the other hand a study by Rajput et al, (2019) reported 22.72% ESBL producing Escherichia coli, 15.9% ESBL producing Klebsiella pneumonia. A study by Zahran et al,reported 88.2% MRSA,62.5% MRCONS, 65.1% ESBL producing Enterobacteriace and 73% carbapenem resistant which is not similar to the present study.…”

A Silent Pandemic of Antimicrobial Resistance: Challenges and Strategy for Preparedness in India

“…coli and 15.9% of Klebsiella were extended-spectrum beta-lactamases producing strains. 13 Similarly, Patel et al also documented increasing emergence of MRSA in 54.8% surgical infections in India. 14…”

Prevalence of Extended Spectrum Beta-Lactamase Producing Gram-Negative Bacilli causing Surgical Site Infections in a Tertiary Care Centre

“…Using the second swab, pus samples were inoculated on Blood agar, MacConkey agar and Thioglycolate broth and were incubated aerobically at 37°C for 18-24 hours. In case of no growth on plates after 24 hours, the respective Thioglycolate broth were examined for turbidity and subcultured if required 11,12 . The bacterial colonies obtained were further processed and identified conventionally, based on colony morphology on culture plates and standard biochemical tests.…”