Bactericidal Activity in Filtrated Supernatant of Streptococcus Sanguinis against Multidrug-Resistant Pseudomonas Aeruginosa

Watanabe, Kiwao; Senba, Masachika; Ichinose, Akitoyo; Yamamoto, Takeshi; Ariyoshi, Koya; Matsumoto, Keizō

doi:10.1620/tjem.219.79

Cited by 10 publications

(10 citation statements)

References 22 publications

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“…Although the prevalence of oral streptococci in CF patients is poorly understood, the ability of oral streptococci to exist in the lungs of these patients and utilize nitrite sources during chronic infection could potentially interfere with P. aeruginosa colonization or virulence. In support of our findings, S. sanguinis isolated from sputum has been shown to exhibit bactericidal activity against 19 nonmucoid and mucoid P. aeruginosa isolates recovered from patients with pulmonary disease, although the bactericidal agent was unknown (26). Other studies have shown that acidified nitrite inhibits P. aeruginosa cells grown under anaerobic conditions (27,28).…”

Section: Discussionsupporting

confidence: 84%

Oral Streptococci and Nitrite-Mediated Interference of Pseudomonas aeruginosa

Scoffield

2015

Infect Immun

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The oral cavity harbors a diverse community of microbes that are physiologically unique. Oral microbes that exist in this polymicrobial environment can be pathogenic or beneficial to the host. Numerous oral microbes contribute to the formation of dental caries and periodontitis; however, there is little understanding of the role these microbes play in systemic infections. There is mounting evidence that suggests that oral commensal streptococci are cocolonized with Pseudomonas aeruginosa during cystic fibrosis pulmonary infections and that the presence of these oral streptococci contributes to improved lung function. The goal of this study was to examine the underlying mechanism by which Streptococcus parasanguinis antagonizes pathogenic P. aeruginosa. In this study, we discovered that oral commensal streptococci, including Streptococcus parasanguinis, Streptococcus sanguinis, and Streptococcus gordonii, inhibit the growth of P. aeruginosa and that this inhibition is mediated by the presence of nitrite and the production of hydrogen peroxide (H 2 O 2 ) by oral streptococci. The requirement of both H 2 O 2 and nitrite for the inhibition of P. aeruginosa is due to the generation of reactive nitrogenous intermediates (RNI), including peroxynitrite. Transposon mutagenesis showed that a P. aeruginosa mutant defective in a putative ABC transporter permease is resistant to both streptococcus/nitrite-and peroxynitrite-mediated killing. Furthermore, S. parasanguinis protects Drosophila melanogaster from killing by P. aeruginosa in a nitrite-dependent manner. Our findings suggest that the combination of nitrite and H 2 O 2 may represent a unique anti-infection strategy by oral streptococci during polymicrobial infections.T he human oral cavity provides residence to more than 700 diverse bacterial species (1). A fraction of the microbial communities that colonize the oral cavity are responsible for localized pathogenic infections, such as dental caries and aggressive periodontitis (1, 2). In contrast, other oral microbes are classified as commensal bacteria (Streptococcus parasanguinis, Streptococcus sanguinis, Streptococcus gordonii, and Streptococcus salivarius) and are involved in facilitating the attachment of pathogenic bacteria to the tooth surface (3) and modulating bacterial dysbiosis (4). Microorganisms unique to the oral cavity have been considered to be mainly involved in localized oral infections; however, there is increasing evidence that suggests that oral microbes may play a more prominent role in systemic infections (5). Oral pathogens and commensals have the ability to disseminate through the bloodstream and cause infective endocarditis (6). Moreover, periodontal pathogens have been associated with cases of atherosclerosis and respiratory tract infections (5, 7).Current evidence suggests that some oral streptococci reside in locations of the body that are distant to the oral cavity, including the respiratory tracts of cystic fibrosis (CF) patients (8). Historically, Pseudomonas aeruginosa was considered to...

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Section: Discussionsupporting

confidence: 84%

Oral Streptococci and Nitrite-Mediated Interference of Pseudomonas aeruginosa

Scoffield

2015

Infect Immun

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“…In the study by Kumar et al Streptococcus and Veillonella also accounted for a significantly greater fraction of the plaques in the healthy individuals (Kumar et al, 2005 ). Streptococcus , one of the earliest and most abundant colonizers of biofilms, can produce an antimicrobial effect against several exogenous pathogens (Watanabe et al, 2009 ), and Veillonella can utilize the lactate produced by the Streptococcus as an energy source (Kuramitsu et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Ecological Shifts of Supragingival Microbiota in Association with Pregnancy

Lin

Jiang

et al. 2018

Front. Cell. Infect. Microbiol.

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Pregnancy is a physiological process with pronounced hormonal fluctuations in females, and relatively little is known regarding how pregnancy influences the ecological shifts of supragingival microbiota. In this study, supragingival plaques and salivary hormones were collected from 11 pregnant women during pregnancy (P1, ≤14 weeks; P2, 20–25 weeks; P3, 33–37 weeks) and the postpartum period (P4, 6 weeks after childbirth). Seven non-pregnant volunteers were sampled at the same time intervals. The microbial genetic repertoire was obtained by 16S rDNA sequencing. Our results indicated that the Shannon diversity in P3 was significantly higher than in the non-pregnant group. The principal coordinates analysis showed distinct clustering according to gestational status, and the partial least squares discriminant analysis identified 33 genera that may contribute to this difference. There were differentially distributed genera, among which Neisseria, Porphyromonas, and Treponema were over-represented in the pregnant group, while Streptococcus and Veillonella were more abundant in the non-pregnant group. In addition, 53 operational taxonomic units were observed to have positive correlations with sex hormones in a redundancy analysis, with Prevotella spp. and Treponema spp. being most abundant. The ecological events suggest that pregnancy has a role in shaping an at-risk-for-harm microbiota and provide a basis for etiological studies of pregnancy-associated oral dysbiosis.

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“…Oral streptococci produces antimicrobials against several exogenous pathogens [57], being responsible for the maintenance of oral microbial homeostasis. On the other hand, oral streptococci are markedly affected by broad-spectrum antibiotics, which are one of the main factors leading to microbial M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 13 dysbiosis, especially in children and elderly [58].…”

Section: Discussionmentioning

confidence: 99%

Periodontal-disease-associated biofilm: A reservoir for pathogens of medical importance

Colombo

Magalhães

Hartenbach

et al. 2016

Microbial Pathogenesis

235

154

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The ecological diversity of the periodontal microenvironment may provide suitable conditions for the colonization of species not usually considered members of the oral microbiota. In this investigation, we aimed to determine the prevalence and levels of pathogenic species of medical relevance in the microbiota of individuals with distinct periodontal clinical status. Subgingival biofilm was obtained from patients with periodontal health (H, n = 81), gingivitis (G, n = 55), generalized aggressive (AgP, n = 36) or chronic periodontitis (CP, n = 98), and analyzed for 39 microbial taxa using a checkerboard DNA-DNA hybridization technique. Microbial differences among groups, as well as associations between clinical and microbiological parameters were sought by non-parametric and univariate correlation tests. Neisseria spp., Peptostreptococus anaerobius, Candida albicans, enterobacteria, Pseudomonas aeruginosa, Eubacterium saphenum, Clostridium difficile and Olsenella uli were detected in high mean prevalence and counts in the subgingival microbiota of the study population. Species that were more related to periodontal inflammation and tissue destruction at the patient and site levels included enterobacteria, C. albicans, Neisseria spp., P. aeruginosa, O. uli, Hafnia alvei, Serratia marcescens and Filifactor alocis (p < 0.05). In contrast, Fusobacterium necrophorum, Lactobacillus acidophilus, Staphylococcus aureus and Streptococcus pneumoniae were associated with periodontal health (p < 0.05). Pathogenic species of medical importance may be detected in high prevalence and levels in the periodontal microbiota. Regardless of their role in periodontal health or disease, the periodontal biofilm may be a source for dissemination and development of systemic infections by these pathogenic microorganisms.

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Bactericidal Activity in Filtrated Supernatant of Streptococcus Sanguinis against Multidrug-Resistant Pseudomonas Aeruginosa

Cited by 10 publications

References 22 publications

Oral Streptococci and Nitrite-Mediated Interference of Pseudomonas aeruginosa

Oral Streptococci and Nitrite-Mediated Interference of Pseudomonas aeruginosa

Ecological Shifts of Supragingival Microbiota in Association with Pregnancy

Periodontal-disease-associated biofilm: A reservoir for pathogens of medical importance

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