Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria

Nakamura, Keisuke; Ishiyama, Kirika; Sheng, Hong; Ikai, Hiroyo; Kanno, Taro; Niwano, Yoshimi

doi:10.1021/jf5058588

Cited by 106 publications

(96 citation statements)

References 33 publications

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“…Recently, Nakamura et al discovered the synergistic effect between aBL and polyphenols, which are naturally occurring polyphenolic compounds in fruits, nuts, and flowers (Nakamura et al, 2015; Nakamura et al, 2012). The polyphenols tested include caffeic acid, gallic acid, chlorogenic acid, epigallocatechin, epigallocatechin gallate, and proanthocyanidin.…”

Section: Synergism Of Antimicrobial Blue Light With Other Agentsmentioning

confidence: 99%

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Wang

et al. 2017

Drug Resistance Updates

223

192

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As an innovative non-antibiotic approach, antimicrobial blue light in the spectrum of 400–470 nm has demonstrated its intrinsic antimicrobial properties resulting from the presence of endogenous photosensitizing chromophores in pathogenic microbes and, subsequently, its promise as a counteracter of antibiotic resistance. Since we published our last review of antimicrobial blue light in 2012, there have been a substantial number of new studies reported in this area. Here we provide an updated overview of the findings from the new studies over the past 5 years, including the efficacy of antimicrobial blue light inactivation of different microbes, its mechanism of action, synergism of antimicrobial blue light with other angents, its effect on host cells and tissues, the potential development of resistance to antimicrobial blue light by microbes, and a novel interstitial delivery approach of antimicrobial blue light. The potential new applications of antimicrobial blue light are also discussed.

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Section: Synergism Of Antimicrobial Blue Light With Other Agentsmentioning

confidence: 99%

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Wang

et al. 2017

Drug Resistance Updates

223

192

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“…A novel antimicrobial technique based on photo-oxidation of polyphenols has been developed in our laboratory 13–16 . Exposing an aqueous solution of polyphenols such as caffeic acid (CA), chlorogenic acid, gallic acid, proanthocyanidin, or crude extract of grape to blue light (wavelength: 400 nm) leads to the photo-oxidation of the polyphenolic hydroxyl group.…”

Section: Introductionmentioning

confidence: 99%

“…This generates hydrogen peroxide (H 2 O 2 ) in the presence of dissolved oxygen 15 . The H 2 O 2 is subsequently photolysed by the same light, resulting in the generation of hydroxyl radicals 13 , which are more potent oxidants than H 2 O 2 17 . When the hydroxyl radicals interact with bacterial cells, they cause lethal oxidative damage such as DNA oxidation and lipid peroxidation 13, 18 .…”

Section: Introductionmentioning

confidence: 99%

“…The H 2 O 2 is subsequently photolysed by the same light, resulting in the generation of hydroxyl radicals 13 , which are more potent oxidants than H 2 O 2 17 . When the hydroxyl radicals interact with bacterial cells, they cause lethal oxidative damage such as DNA oxidation and lipid peroxidation 13, 18 . Since hydroxyl radicals are generated only at the time and place of light irradiation, the area and antimicrobial activity of the treatment are controllable.…”

Section: Introductionmentioning

confidence: 99%

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Photo-irradiated caffeic acid exhibits antimicrobial activity against Streptococcus mutans biofilms via hydroxyl radical formation

Nakamura

Shirato

Kanno

et al. 2017

Sci Rep

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An antimicrobial technique based on photo-oxidation of caffeic acid (CA) has recently been developed, but its effect on biofilm-forming bacteria is unknown. The present study aimed to evaluate the effect of photo-irradiated CA against Streptococcus mutans (cariogenic bacteria) biofilm as it relates to hydroxyl radical formation. S. mutans biofilms grown on hydroxyapatite disks were immersed in CA solution (0–2 mg/mL) and irradiated with LED light at wavelengths of 365, 385, and 400 nm and at irradiances of 500, 1000, and 2000 mW/cm2 for 4 min. Biofilm viable bacterial counts were determined by colony counting. The yield of hydroxyl radicals generated by the LED irradiation of CA solution was quantified by electron spin resonance analysis. Of the conditions tested, the highest bactericidal effect, with a > 5-log reduction in viable bacterial counts, was obtained by irradiation of a 1 mg/mL CA solution with 385 nm LED and at an irradiance of 2000 mW/cm2. Hydroxyl radical formation was related to this bactericidal effect. The present study suggests that the antimicrobial technique based on the 385 nm LED irradiation of CA is effective against cariogenic biofilms and can be applied as an adjunctive chemotherapy for dental caries.

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“…It was reported that an important anticancer mechanism of plant polyphenols is mediated through intracellular copper mobilization and reactive oxygen species (ROS) generation, which is a characteristic feature of pro-oxidant properties of polyphenolic compounds, leading to cancer cell death (Khan et al 2014). In our previous studies, the pro-oxidant potential of polyphenols was applied to the development of a novel disinfection technique (Nakamura et al 2012b(Nakamura et al , 2013(Nakamura et al , 2015. Exposing an aqueous solution of polyphenols to blue light led to photooxidation of the polyphenolic hydroxyl group, resulting in the generation of hydrogen peroxide (H 2 O 2 ) produced via electron transfer from photooxidized polyphenols to dissolved oxygen.…”

Section: Introductionmentioning

confidence: 99%

Microbicidal action of photoirradiated aqueous extracts from wine lees

et al. 2016

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Wine lees, a major waste product of winemaking, is a rich source of polyphenolic compounds. LED-light irradiation at 400-nm elicited microbicidal activity of aqueous extract from wine lees (WLE) against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans, in addition to reactive oxygen species (ROS) formation, including hydroxyl radical (ÁOH) and hydrogen peroxide (H 2 O 2 ). Although treatment for 20 min of photoirradiation alone exerted bactericidal activity with a 2-to 3-log reduction, photoirradiated WLE for 20 min achieved a 5-log or greater reduction in viable S. aureus and P. aeruginosa cells. Regarding C. albicans, a 1-log reduction (90 % reduction) of viable cells was achieved by photoirradiated WLE for 40 min, whereas photoirradiation alone did not show any fungicidal effect. ROS analyses revealed that approximately 170 lM ÁOH and 600 lM H 2 O 2 were generated in photoirradiated WLE for 20 min. Because the bactericidal activity of photoirradiated WLE was abolished by ÁOH scavengers, ROS, especially highly oxidative ÁOH, may be responsible for the microbicidal activity of photoirradiated WLE. In addition to its microbicidal activity, WLE may act as an antioxidant as it exerted radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl, a stable free radical.

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Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria

Cited by 106 publications

References 33 publications

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Antimicrobial blue light inactivation of pathogenic microbes: State of the art

Photo-irradiated caffeic acid exhibits antimicrobial activity against Streptococcus mutans biofilms via hydroxyl radical formation

Microbicidal action of photoirradiated aqueous extracts from wine lees

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