Bactericidal Action of Photoirradiated Gallic Acid via Reactive Oxygen Species Formation

Nakamura, Keisuke; Yamada, Yukie; Ikai, Hiroyo; Kanno, Taro; Sasaki, Keiichi; Niwano, Yoshimi

doi:10.1021/jf303177p

Cited by 85 publications

(95 citation statements)

References 38 publications

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“…9−13 In our previous study, it was demonstrated that the antimicrobial activity of polyphenols, such as gallic acid and proanthocyanidin, was considerably enhanced by blue light irradiation at a wavelength of around 400 nm. 14,15 When Staphylococcus aureus suspended in an aqueous solution of the polyphenols was exposed to blue light, they were killed with a >5 log reduction of viable counts within 15−30 min.…”

Section: ■ Introductionmentioning

confidence: 99%

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Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria

Nakamura

Ishiyama

Sheng

et al. 2015

J. Agric. Food Chem.

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106

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The bactericidal effect of various types of photoirradiated polyphenols against Gram-positive and -negative bacteria was evaluated in relation to the mode of action. Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus, and Streptococcus mutans) and Gram-negative bacteria (Aggregatibacter actinomycetemcomitans, Escherichia coli, and Pseudomonas aeruginosa) suspended in a 1 mg/mL polyphenol aqueous solution (caffeic acid, gallic acid, chlorogenic acid, epigallocatechin, epigallocatechin gallate, and proanthocyanidin) were exposed to LED light (wavelength, 400 nm; irradiance, 260 mW/cm(2)) for 5 or 10 min. Caffeic acid and chlorogenic acid exerted the highest bactericidal activity followed by gallic acid and proanthocyanidin against both Gram-positive and -negative bacteria. It was also demonstrated that the disinfection treatment induced oxidative damage of bacterial DNA, which suggests that polyphenols are incorporated into bacterial cells. The present study suggests that blue light irradiation of polyphenols could be a novel disinfection treatment.

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Section: ■ Introductionmentioning

confidence: 99%

“…14 Hydrogen peroxide (H 2 O 2 ) is produced via electron transfer from photo-oxidized polyphenols to dissolved oxygen. The H 2 O 2 is subsequently photolyzed by the blue light, resulting in the generation of hydroxyl radicals ( • OH), 16 which would be a main contributor of the bactericidal activity.…”

Section: ■ Introductionmentioning

confidence: 99%

Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria

Nakamura

Ishiyama

Sheng

et al. 2015

J. Agric. Food Chem.

Self Cite

106

View full text Add to dashboard Cite

show abstract

“…17.2). Nakamura et al demonstrated that hydroxyl radicals were generated when gallic acid, a polyphenolic compound, was irradiated with blue light (wavelength: 400 nm) at an irradiance of 80 mW/cm 2 [72]. It was also demonstrated that S. aureus was killed by the photo-irradiation of gallic acid with a >5-log reduction of viable counts within 15 min.…”

Section: Other Hydroxyl Radical Generation Systemsmentioning

confidence: 99%

Microbicidal Activity of Artificially Generated Hydroxyl Radicals

Sheng

Nakamura

Kanno

et al. 2015

Interface Oral Health Science 2014

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The hydroxyl radical, one of the reactive oxygen species, has one unpaired electron in the structure, so that it tends to deprive other substances of an electron which is so-called oxidation. It is known that hydroxyl radicals produced by immunological response kill invading microorganisms by the oxidation. Besides the immune system, it has been demonstrated that hydroxyl radicals play an important role in the bactericidal action of antibiotics. In this context, we have conducted a research to develop disinfection techniques utilizing artificially generated hydroxyl radicals. We adopted photolysis of H 2 O 2 , sonolysis of water and the other photo-chemical reaction as generators of hydroxyl radicals. A series of studies demonstrated that the microbicidal activity of hydroxyl radicals was sufficient to kill bacteria in an experimental biofilm as well as planktonic bacteria and fungi within a short-treatment time. In addition, the safety aspect is confirmed by an in vivo study and a literature review. Thus, it is suggested that disinfection treatment utilizing artificially generated hydroxyl radicals can be applicable to medical/dental therapy as novel disinfection treatments.

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“…It was reported that an important anticancer mechanism of plant polyphenols is mediated through intracellular copper mobilization and reactive oxygen species (ROS) generation, which is a characteristic feature of pro-oxidant properties of polyphenolic compounds, leading to cancer cell death (Khan et al 2014). In our previous studies, the pro-oxidant potential of polyphenols was applied to the development of a novel disinfection technique (Nakamura et al 2012b(Nakamura et al , 2013(Nakamura et al , 2015. Exposing an aqueous solution of polyphenols to blue light led to photooxidation of the polyphenolic hydroxyl group, resulting in the generation of hydrogen peroxide (H 2 O 2 ) produced via electron transfer from photooxidized polyphenols to dissolved oxygen.…”

Section: Introductionmentioning

confidence: 99%

Microbicidal action of photoirradiated aqueous extracts from wine lees

et al. 2016

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Wine lees, a major waste product of winemaking, is a rich source of polyphenolic compounds. LED-light irradiation at 400-nm elicited microbicidal activity of aqueous extract from wine lees (WLE) against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans, in addition to reactive oxygen species (ROS) formation, including hydroxyl radical (ÁOH) and hydrogen peroxide (H 2 O 2 ). Although treatment for 20 min of photoirradiation alone exerted bactericidal activity with a 2-to 3-log reduction, photoirradiated WLE for 20 min achieved a 5-log or greater reduction in viable S. aureus and P. aeruginosa cells. Regarding C. albicans, a 1-log reduction (90 % reduction) of viable cells was achieved by photoirradiated WLE for 40 min, whereas photoirradiation alone did not show any fungicidal effect. ROS analyses revealed that approximately 170 lM ÁOH and 600 lM H 2 O 2 were generated in photoirradiated WLE for 20 min. Because the bactericidal activity of photoirradiated WLE was abolished by ÁOH scavengers, ROS, especially highly oxidative ÁOH, may be responsible for the microbicidal activity of photoirradiated WLE. In addition to its microbicidal activity, WLE may act as an antioxidant as it exerted radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl, a stable free radical.

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Bactericidal Action of Photoirradiated Gallic Acid via Reactive Oxygen Species Formation

Cited by 85 publications

References 38 publications

Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria

Bactericidal Activity and Mechanism of Photoirradiated Polyphenols against Gram-Positive and -Negative Bacteria

Microbicidal Activity of Artificially Generated Hydroxyl Radicals

Microbicidal action of photoirradiated aqueous extracts from wine lees

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