Bacterial poly(A) polymerase: An enzyme that modulates RNA stability

The Bacillus subtilis Nucleotidyltransferase Is a tRNA CCA-Adding Enzyme

“…Our antiserum against E. coli PAP I was previously shown to detect proteins in several other gram-negative bacteria: Yersinia pseudotuberculosis, Erwinia carotovora, P. mirabilis, and D. gigas (39). We also detected a related protein in B. subtilis, a member of the low-GϩC gram-positive family of bacteria (Fig.…”

“…1C). By Western blotting, the B. subtilis protein, with a mass of 55 kDa, reacted weakly with the antibody against E. coli PAP I, requiring a 10-fold-longer exposure for detection by chemiluminescence (39). The 45-kDa protein characterized here, which is the B. subtilis tRNA nucleotidyltransferase, does not react with the antibody against E. coli PAP I.…”

“…The faintly detected protein in Fig. 3B, lane 2, which is slightly larger than the overexpressed PAP I, is the B. subtilis protein that cross-reacts with the PAP I antibody (39). Note that PapS (Fig.…”

The Bacillus subtilis Nucleotidyltransferase Is a tRNA CCA-Adding Enzyme

“…Our antiserum against E. coli PAP I was previously shown to detect proteins in several other gram-negative bacteria: Yersinia pseudotuberculosis, Erwinia carotovora, P. mirabilis, and D. gigas (39). We also detected a related protein in B. subtilis, a member of the low-GϩC gram-positive family of bacteria (Fig.…”

“…1C). By Western blotting, the B. subtilis protein, with a mass of 55 kDa, reacted weakly with the antibody against E. coli PAP I, requiring a 10-fold-longer exposure for detection by chemiluminescence (39). The 45-kDa protein characterized here, which is the B. subtilis tRNA nucleotidyltransferase, does not react with the antibody against E. coli PAP I.…”

“…The faintly detected protein in Fig. 3B, lane 2, which is slightly larger than the overexpressed PAP I, is the B. subtilis protein that cross-reacts with the PAP I antibody (39). Note that PapS (Fig.…”

Closely Related CC- and A-adding Enzymes Collaborate to Construct and Repair the 3′-Terminal CCA of tRNA in Synechocystis sp. and Deinococcus radiodurans

“…Because it is not yet possible to distinguish Class II CCA-adding enzymes from poly(A) polymerases based solely on sequence analysis (38), we included only those enzymes whose enzymatic activities had been experimentally demonstrated or could be confidently assumed. These are the H. influenzae CCAadding enzyme and poly(A) polymerase, both of which are almost identical to their experimentally characterized E. coli counterparts (21,23,39), the B. subtilis and M. leprae CCA-adding enzymes (38), and the CCA-adding enzyme from M. tuberculosis, which is almost identical to M. leprae enzyme (31). A multiple alignment of the 25-kDa core regions of these Class II enzymes, including the active site signature, is shown in Fig.…”

The Sm‐like protein Hfq regulates polyadenylation dependent mRNA decay in Escherichia coli

“…Besides the two different monomeric forms of PAP I, some multimeric species were also observed (Fig. 5A, stars; Raynal et al ., 1996).…”

The Bacillus subtilis Nucleotidyltransferase Is a tRNA CCA-Adding Enzyme

“…Our antiserum against E. coli PAP I was previously shown to detect proteins in several other gram-negative bacteria: Yersinia pseudotuberculosis, Erwinia carotovora, P. mirabilis, and D. gigas (39). We also detected a related protein in B. subtilis, a member of the low-GϩC gram-positive family of bacteria (Fig.…”

“…1C). By Western blotting, the B. subtilis protein, with a mass of 55 kDa, reacted weakly with the antibody against E. coli PAP I, requiring a 10-fold-longer exposure for detection by chemiluminescence (39). The 45-kDa protein characterized here, which is the B. subtilis tRNA nucleotidyltransferase, does not react with the antibody against E. coli PAP I.…”

“…The faintly detected protein in Fig. 3B, lane 2, which is slightly larger than the overexpressed PAP I, is the B. subtilis protein that cross-reacts with the PAP I antibody (39). Note that PapS (Fig.…”

Closely Related CC- and A-adding Enzymes Collaborate to Construct and Repair the 3′-Terminal CCA of tRNA in Synechocystis sp. and Deinococcus radiodurans

“…Because it is not yet possible to distinguish Class II CCA-adding enzymes from poly(A) polymerases based solely on sequence analysis (38), we included only those enzymes whose enzymatic activities had been experimentally demonstrated or could be confidently assumed. These are the H. influenzae CCAadding enzyme and poly(A) polymerase, both of which are almost identical to their experimentally characterized E. coli counterparts (21,23,39), the B. subtilis and M. leprae CCA-adding enzymes (38), and the CCA-adding enzyme from M. tuberculosis, which is almost identical to M. leprae enzyme (31). A multiple alignment of the 25-kDa core regions of these Class II enzymes, including the active site signature, is shown in Fig.…”

The Sm‐like protein Hfq regulates polyadenylation dependent mRNA decay in Escherichia coli

“…Besides the two different monomeric forms of PAP I, some multimeric species were also observed (Fig. 5A, stars; Raynal et al ., 1996).…”

The Bacillus subtilis Nucleotidyltransferase Is a tRNA CCA-Adding Enzyme

“…Our antiserum against E. coli PAP I was previously shown to detect proteins in several other gram-negative bacteria: Yersinia pseudotuberculosis, Erwinia carotovora, P. mirabilis, and D. gigas (39). We also detected a related protein in B. subtilis, a member of the low-GϩC gram-positive family of bacteria (Fig.…”

“…1C). By Western blotting, the B. subtilis protein, with a mass of 55 kDa, reacted weakly with the antibody against E. coli PAP I, requiring a 10-fold-longer exposure for detection by chemiluminescence (39). The 45-kDa protein characterized here, which is the B. subtilis tRNA nucleotidyltransferase, does not react with the antibody against E. coli PAP I.…”

“…The faintly detected protein in Fig. 3B, lane 2, which is slightly larger than the overexpressed PAP I, is the B. subtilis protein that cross-reacts with the PAP I antibody (39). Note that PapS (Fig.…”

Closely Related CC- and A-adding Enzymes Collaborate to Construct and Repair the 3′-Terminal CCA of tRNA in Synechocystis sp. and Deinococcus radiodurans

“…Because it is not yet possible to distinguish Class II CCA-adding enzymes from poly(A) polymerases based solely on sequence analysis (38), we included only those enzymes whose enzymatic activities had been experimentally demonstrated or could be confidently assumed. These are the H. influenzae CCAadding enzyme and poly(A) polymerase, both of which are almost identical to their experimentally characterized E. coli counterparts (21,23,39), the B. subtilis and M. leprae CCA-adding enzymes (38), and the CCA-adding enzyme from M. tuberculosis, which is almost identical to M. leprae enzyme (31). A multiple alignment of the 25-kDa core regions of these Class II enzymes, including the active site signature, is shown in Fig.…”

The Sm‐like protein Hfq regulates polyadenylation dependent mRNA decay in Escherichia coli

“…Besides the two different monomeric forms of PAP I, some multimeric species were also observed (Fig. 5A, stars; Raynal et al ., 1996).…”