Bacterial nanocellulose membranes combined with nisin: a strategy to prevent microbial growth

Santos, Carolina Alves dos; Santos, Gabriela R. dos; Soeiro, Victória Soares; Santos, Júlia Rodrigues dos; Rebelo, Márcia de Araújo; Chaud, Marco V.; Gerenutti, Marli; Grotto, Denise; Pandit, Raksha; Rai, Mahendra; Jozala, Ângela Faustino

doi:10.1007/s10570-018-2010-1

Cited by 46 publications

(29 citation statements)

References 48 publications

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“…Antimicrobial BC membranes have been obtained by Santos et al (2018), by impregnating BC sheets into nisin. FTIR revealed the immobilization of nisin to BC, which was ascribed to linkings of amine groups of nisin to C6 carboxylic acid groups of BC.…”

Section: Impregnation With Active Compoundsmentioning

confidence: 99%

Bacterial Cellulose as a Raw Material for Food and Food Packaging Applications

Azeredo

Barud

Farinas

et al. 2019

Front. Sustain. Food Syst.

348

221

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Bacterial cellulose (BC) has been produced for a number of applications, mainly focused on the biomedical area. Although there is a variety of interesting applications of BC for food and food packaging, only a few have been explored to the moment, since the high cost of BC production is usually considered as a limiting factor. On the other hand, several cost-effective culture media have been proposed, contributing to reduce BC production costs. This article overviews the bioprocess conditions that affects BC production and the main possible applications of BC for food and food packaging purposes.

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Section: Impregnation With Active Compoundsmentioning

confidence: 99%

Bacterial Cellulose as a Raw Material for Food and Food Packaging Applications

Azeredo

Barud

Farinas

et al. 2019

Front. Sustain. Food Syst.

348

221

View full text Add to dashboard Cite

show abstract

“…Bacterial nanocellulose films with adsorbed biomacromolecules such as the already-mentioned nisin or lactoferrin were fabricated to reduce most frequently met foodborne pathogens as E. coli , S. aureus, and L. monocytogenes [ 92 , 93 , 113 ]. Dos Santos et al [ 113 ] developed composites that could be used in an active food packing system by impregnating BNC films in nisin-EDTA solution. The agar disc diffusion assay showed inhibition zones around the tested samples, which indicated antimicrobial effect against two pathogenic strains E. coli and S. aureus .…”

Section: Bnc In Food Packaging Applicationsmentioning

confidence: 99%

“…Furthermore, it was found that the combination of nisin with chelating agent (EDTA) resulted in even stronger inhibition of bacterial cell wall synthesis. BNC/nisin films also exhibited antioxidant activity, which was examined by DPPH radical scavenging assay [ 113 ]. Another approach to produce this type of food packages is the employment of antimicrobial enzymes.…”

Section: Bnc In Food Packaging Applicationsmentioning

confidence: 99%

Bacterial Nanocellulose—A Biobased Polymer for Active and Intelligent Food Packaging Applications: Recent Advances and Developments

2020

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The aim of this review is to provide an overview of recent findings related to bacterial cellulose application in bio-packaging industry. This constantly growing sector fulfils a major role by the maintenance of product safety and quality, protection against environmental impacts that affect the shelf life. Conventional petroleum-based plastic packaging are still rarely recyclable and have a number of harmful environmental effects. Herein, we discuss the most recent studies on potential good alternative to plastic packaging—bacterial nanocellulose (BNC), known as an ecological, safe, biodegradable, and chemically pure biopolymer. The limitations of this bio-based packaging material, including relatively poor mechanical properties or lack of antimicrobial and antioxidant activity, can be successfully overcome by its modification with a wide variety of bioactive and reinforcing compounds. BNC active and intelligent food packaging offer a new and innovative approach to extend the shelf life and maintain, improve, or monitor product quality and safety. Incorporation of different agents BNC matrices allows to obtain e.g., antioxidant-releasing films, moisture absorbers, antimicrobial membranes or pH, freshness and damage indicators, humidity, and other biosensors. However, further development and implementation of this kind of bio-packaging will highly depend on the final performance and cost-effectiveness for the industry and consumers.

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“…They do not need chemical modification of either the matrix or the enzyme, allowing minimal configuration change of the enzyme (Choi 2004). Enzyme immobilization by physical adsorption has been described for lysozyme onto BC fibres in suspension (Bayazidi et al 2018), for lipase onto BC nanocrystals (Kim et al 2015) and for nisin, laccase and lipase onto BC membranes (Wu et al 2017;Yuan et al 2018;dos Santos et al 2018). Nevertheless, obtaining an enzymatically active BC nanocomposite that had the physical characteristics and the handiness of the paper would be of great interest.…”

Section: Introductionmentioning

confidence: 99%

Bacterial cellulose matrices to develop enzymatically active paper

et al. 2020

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This work studies the suitability of bacterial cellulose (BC) matrices to prepare enzymatically active nanocomposites, in a framework of more environmentally friendly methodologies. After BC production and purification, two kind of matrices were obtained: BC in aqueous suspension and BC paper. A lipase was immobilised onto the BC matrices by physical adsorption, obtaining Lipase/BC nanocomposites. Neither morphology nor crystallinity, measured by scanning electron microscopy (SEM) and X-Ray diffractometry (XRD) respectively, of the BC were affected by the binding of the protein. The activity of Lipase/BC suspension and Lipase/BC paper was tested under different conditions, and the operational properties of the enzyme were evaluated. A shift towards higher temperatures, a broader pH activity range, and slight differences in the substrate preference were observed in the immobilised lipase, compared with the free enzyme. Specific activity was higher for Lipase/BC suspension (4.2 U/mg) than for Lipase/BC paper (1.7 U/mg) nanocomposites. However, Lipase/BC paper nanocomposites showed improved thermal stability, reusability, and durability. Enzyme immobilised onto BC paper retained 60% of its activity after 48 h at 60 ºC. It maintained 100% of the original activity after being recycled 10 times at pH 7 at 60 ºC and it remained active after being stored for more than a month at room temperature. The results suggested that lipase/BC nanocomposites are promising biomaterials for the development of green biotechnological devices with potential application in industrials bioprocesses of detergents and food industry and biomedicine. Lipase/BC paper nanocomposite might be a key component of bioactive paper for developing simple, handheld, and disposable devices.

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Bacterial nanocellulose membranes combined with nisin: a strategy to prevent microbial growth

Cited by 46 publications

References 48 publications

Bacterial Cellulose as a Raw Material for Food and Food Packaging Applications

Bacterial Cellulose as a Raw Material for Food and Food Packaging Applications

Bacterial Nanocellulose—A Biobased Polymer for Active and Intelligent Food Packaging Applications: Recent Advances and Developments

Bacterial cellulose matrices to develop enzymatically active paper

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