Bacterial microbiome in Armillaria ostoyae rhizomorphs inhabiting the root zone during progressively dying Scots pine

Przemieniecki, Sebastian Wojciech; Damszel, Marta; Ciesielski, Sławomir; Kubiak, Katarzyna; Mastalerz, Jędrzej; Sierota, Z.; Gorczyca, Anna

doi:10.1016/j.apsoil.2021.103929

Cited by 16 publications

(15 citation statements)

References 52 publications

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“…From the results obtained by Siebyła and Hilszczańska (2020) on the identification of soil bacterial communities, it can be concluded that the weather conditions (here: in the autumn season of 2017) resulted in an increase in the amount of bacterial population. Similar relationships are described by Przemieniecki et al (2021), who point to the significant influence of weather conditions at different times of the year on the activity of the bacterial microbiome in Armillaria ostoyae rhizomorphs inhabiting the roots of Scots pine trees.…”

Section: Discussionsupporting

confidence: 78%

Comparison of bacterial communities in roots of selected trees with and without summer truffle (Tuber aestivum) ectomycorrhiza

Siebyła

Szyp-Borowska

2021

Folia Forestalia Polonica

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In this study, we examined the effect of the presence of mycorrhiza and ascomata of summer truffle (Tuber aestivum) on the bacterial composition of roots from small trees growing in selected sites of the Nida Basin. Qualitative DNA sequencing methods such as Sanger and next-generation sequencing (NGS) were used. The Sanger method revealed different bacterial species compositions between the samples where summer truffle ascomata was recorded and control samples. Five genera of bacteria could be distinguished: Bacillus, Erwinia, Pseudomonas, Rahnella and Serratia, among which the most numerous were Pseudomonas (Gammmaproteobacteria class) at 32.9%. The results obtained by the NGS method also showed differences in species composition of the bacteria depending on the study sample. Seven genera of bacteria were distinguished: Rhizorhabdus, Methylotenera, Sphingomonas, Nitrosospira, Streptomyces, Methyloceanibacter and Niastella, which dominated in roots from the truffle sites. Telmatobacter, Roseiarcus, Granulicella, Paludibaculum, Acidipila, Acidisphaera and Aliidongia dominated in roots from the control sites. With the NGS method, it is possible to identify the microbiome of a whole root, while only a root fragment can be analysed by the Sanger method. These results extend the scope of knowledge on the preferences of certain groups of bacteria associated with truffles and their influence on the formation of ascomata in summer truffles. Our results may also be useful in selecting and monitoring sites that promote ascomata of Tuber aestivum.

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Section: Discussionsupporting

confidence: 78%

Comparison of bacterial communities in roots of selected trees with and without summer truffle (Tuber aestivum) ectomycorrhiza

Siebyła

Szyp-Borowska

2021

Folia Forestalia Polonica

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“…The Armillaria species found here (A. cepistipes and A. borealis) are mainly described as weak pathogens of deciduous trees and as opportunistic pathogens or saprotrophs of conifers [96][97][98][99]. In the current study, A. cepistipes was identified by genetic analyses of DNA from underneath bark, rhizomorphs, and basidiocarps collected in all stands studied, but surprisingly, more frequently in stands established on post-agricultural soils than on forest soils, which is described by other authors [100][101][102].…”

Section: Discussionsupporting

confidence: 73%

Fungi Occurring in Norway Spruce Wood Decayed by Heterobasidion parviporum in Puszcza Borecka Stands (Northeastern Poland)

Szczepkowski

Kowalczuk²,

Sikora

et al. 2022

Forests

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In many spruce stands, trees are frequently attacked by the pathogen Heterobasidion parviporum, albeit without visible symptoms in the crown. In the present work, the results of the presence of stem rot, assessed by PICUS Sonic Tomography, and the fungal biota on trees and stumps in eight plots in the Puszcza Borecka Forest are described. The plots were located in stands on original forest soil (4) and on post-agricultural soil (4), where around a stump with H. parviporum symptoms (signs of internal rot and basidiocarps), 30 trees were selected and examined for internal rot. Wood samples were collected from two selected trees for fungal molecular analysis. A total of 79 fungal taxa were found, including 57 taxa in plots on post-agricultural soil and 45 on forest soil. There were 395 fungal records on stumps and 22 records on trees, therein, from the inner parts of felled trunks. Significant differences in the Chao-1 diversity index indicate that the origin of the soil—post-agricultural or forest soil—influenced the alpha diversity of the fungal communities in the forests studied. The values of the Shannon and Simpson indices show that the two communities were similar in terms of species numbers. The presence of basidiomata of H. parviporum and two species of Armillaria (mainly A. cepistipes) in samples on all plots is striking, although Armillaria spp. was detected more frequently. Most of the species identified were typical saprotrophs, although rare species were also found, such as Entoloma byssisedum, Onnia tomentosa, Physisporinus vitreus, Postia ptychogaster, and Ramaria apiculata. The presence of H. parviporum in the inner woody parts was confirmed by PCR analysis, and decay was detected even up to a stem height of 6 m. Armillaria was the dominant genus in the studied stands and plays a significant and underestimated role in heartwood decay of old spruce trees in Puszcza Borecka Forest.

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“…Similar to our study, however, Byers et al [120] found that Solirubrobacterales were more abundant with trees in decline. Przemieniecki et al [121] surveyed bacterial communities associated A. ostoyae rhizomorphs during three stages of tree decline. They observed that rhizomorphs that were rich in Parabacteriodes, Clostridium, and Bacillus were able to hydrolyze diverse organic compounds that could assist Armillaria rhizomorph enzymes in wood decomposition.…”

Section: Discussionmentioning

confidence: 99%

Genomic Comparisons of Two Armillaria Species with Different Ecological Behaviors and Their Associated Soil Microbial Communities

et al. 2022

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Armillaria species show considerable variation in ecological roles and virulence, from mycorrhizae and saprophytes to important root pathogens of trees and horticultural crops. We studied two Armillaria species that can be found in coniferous forests of northwestern USA and southwestern Canada. Armillaria altimontana is considered as a weak, opportunistic pathogen of coniferous trees, but it also appears to exhibit in situ biological control against A. solidipes, formerly North American A. ostoyae, which is considered a virulent pathogen of coniferous trees. Here, we describe their genome assemblies and present a functional annotation of the predicted genes and proteins for the two Armillaria species that exhibit contrasting ecological roles. In addition, the soil microbial communities were examined in association with the two Armillaria species within a 45-year-old plantation of western white pine (Pinus monticola) in northern Idaho, USA, where A. altimontana was associated with improved tree growth and survival, while A. solidipes was associated with reduced growth and survival.ostoyae) is considered one of the more virulent pathogens [13], although virulence varies depending on isolate, host age and other factors [14]. Armillaria mellea and A. borealis are also considered virulent pathogens, while A. gallica, A. cepistipes, A. gemina, A. calvescens, A. sinapina, and A. nabsnona are considered less virulent or secondary pathogens [10, 12,15, 16]. A recently described species, Armillaria altimontana, formerly North American biological species (NABS) X, is also usually considered as a weak pathogen [17], but evidence for pathogenicity is not well documented [18]. Armillaria solidipes (as A. ostoyae) and A. altimontana have been documented to co-occur within forest stands in the inland northwestern USA [18][19][20]. A previous study in northern Idaho, USA provided evidence that A. altimontana can provide natural biological control of Armillaria root disease of western white pine (Pinus monticola) caused by A. solidipes. In this study, A. solidipes was uncommon in areas dominated by A. altimontana, and trees colonized by A. solidipes were associated with a lower growth and survival than trees colonized only by A. altimontana. The results demonstrated that A. altimontana was not harmful to western white pine within the northern Idaho planting site, and further suggest that A. altimontana behaves as a long-term, in situ biological control agent against A. solidipes [20]. Recognizing the genetic or underlying soil factors that drive host-fungal interactions may provide approaches for enhancing the management of Armillaria root disease.The distribution, life cycle, pathogenicity, and evolutionary relationships have been studied for several Armillaria species [10, 12,14, 16,[21][22][23][24][25]. Collins et al. [26] studied the genome and proteome of A. mellea, identifying carbohydrate degrading enzymes, laccases, and lignin peroxidases among other geneencoded proteins. characterized the transcriptome of an A. solidipe...

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Bacterial microbiome in Armillaria ostoyae rhizomorphs inhabiting the root zone during progressively dying Scots pine

Cited by 16 publications

References 52 publications

Comparison of bacterial communities in roots of selected trees with and without summer truffle (Tuber aestivum) ectomycorrhiza

Comparison of bacterial communities in roots of selected trees with and without summer truffle (Tuber aestivum) ectomycorrhiza

Fungi Occurring in Norway Spruce Wood Decayed by Heterobasidion parviporum in Puszcza Borecka Stands (Northeastern Poland)

Genomic Comparisons of Two Armillaria Species with Different Ecological Behaviors and Their Associated Soil Microbial Communities

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