Bacterial lipopolysaccharide-induced oxidative stress in the impairment of steroidogenesis and spermatogenesis in rats

Reddy, M Mallikarjuna; Mahipal, Suraneni V.K.; Jagu, Subhashini; Reddy, Madhava C.; Roy, Kunal; Reddy, Gorla V.; Reddy, P. Raveendra; Reddanna, Pallu

doi:10.1016/j.reprotox.2006.03.003

Cited by 131 publications

(104 citation statements)

References 42 publications

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“…Decreased GSH levels and antioxidant enzymes such as catalase have been identified in the process of LPS treatment. 21) In our results, both GSH levels and catalase activity decreased in LPS-induced inflammatory liver. In the groups pretreated with HPE, the GSH levels and catalase activity improved significantly.…”

Section: Discussionsupporting

confidence: 61%

“…Male Sprague-Dawley rats (260 AE 10 g), used in the studies, were purchased from the National Laboratory Animal Breeding and Research Center (Taipei, Taiwan). The animals were housed under laboratory conditions (18)(19)(20)(21)(22)(23) C, humidity 55-60%, 12 h light/dark cycle) for at least 1 week before each study. All animal studies were conducted according to the guidelines for care and use of laboratory animals approved by the Institute of Animal Care and Use Committee (IACUC) of Chung Shan Medical University.…”

Section: Methodsmentioning

confidence: 99%

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Polyphenols Extracted fromHibiscus sabdariffaL. Inhibited Lipopolysaccharide-Induced Inflammation by Improving Antioxidative Conditions and RegulatingCyclooxygenase-2 Expression

Kao

Hsu

Wang

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionsupporting

confidence: 61%

Section: Methodsmentioning

confidence: 99%

Polyphenols Extracted fromHibiscus sabdariffaL. Inhibited Lipopolysaccharide-Induced Inflammation by Improving Antioxidative Conditions and RegulatingCyclooxygenase-2 Expression

Kao

Hsu

Wang

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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“…Emerging evidence has suggested a possible role of NO in the inhibition of STAR protein expression in Leydig cells (Diemer et al 2003, Herman & Rivier 2006, Reddy et al 2006, indicating the existence of at least one additional site of action of NO in the regulation of steroidogenesis (Del Punta et al 1996). In agreement with the results obtained for NO, HO induction by hemin inhibited STAR protein expression, suggesting that CO may also regulate steroidogenesis by modulating cholesterol transport to the inner mitochondrial membrane.…”

Section: Discussionsupporting

confidence: 79%

Effects of heme oxygenase isozymes on Leydig cells steroidogenesis

Piotrkowski¹,

Monzón²,

Pagotto³

et al. 2009

Journal of Endocrinology

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In the present study, we demonstrate the expression of heme oxygenase (HO) isozymes, HO-1 and HO-2 (listed as HMOX1 and HMOX2 in the MGI Database), in MA-10 Leydig tumor cells and its effect on steroidogenesis. The wellknown HO inducer, hemin, increased both HO-1 and HO-2 protein levels and HO-specific activity. Induction of HO by hemin inhibited basal, hCG-, and dibutyryl cAMP (db-cAMP)-induced steroidogenesis in a reversible way. When we studied the effect of HO isozymes along the steroid synthesis, we found that steroidogenic acute regulatory protein levels were decreased, and the conversion of cholesterol to pregnenolone was inhibited by hemin treatment, with no changes in the content of cholesterol side-chain cleavage enzyme (P450scc). hCG and db-cAMP also stimulated the expression of HO-1 and HO-2, and HO enzymatic activity in MA-10 cells. Basal and hCG-stimulated testosterone synthesis was also inhibited by hemin in rat normal Leydig cells. Taken together, these results suggest that: i) at least one of HO products (presumably carbon monoxide) inhibits cholesterol transport to the inner mitochondrial membrane and Leydig cell steroidogenesis by binding to the heme group of the cytochrome P450 enzymes, in a similar way as we described for nitric oxide, and ii) hCG stimulation results in the induction of an antioxidant enzymatic system (HO) acting as a cytoprotective mechanism in Leydig cells, as already demonstrated in the adrenal gland.

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“…This oxidative stress was associated with the transient generation of pro-inflammatory mediators such as interleukin 1β, inducible nitric oxide synthase and cyclo-oxygenase-2. 88 The same experimental infection model has also been used to demonstrate the particular sensitivity of Leydig cell steroidogenesis to oxidative stress induced by bacterial LPS. In these studies, the oxidative stress induced by LPS stimulated lipid peroxidation in Leydig cell membranes as well as significant reductions in steroidogenic acute regulatory protein (StAR) and 3β-hydroxysteroid dehydrogenase isomerase (3β-HSD) activity.…”

Section: Disruption Of the Antioxidant Status Of The Testesmentioning

confidence: 99%