Bacterial Inositol Catabolism—A Sweet Ride into the Host

Kohler, Petra R. A.; Rossbach, Silvia

doi:10.1002/9781118297674.ch111

Cited by 1 publication

(1 citation statement)

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“…The first compound has been previously described as cytotoxic against B-lymphoblast and T-cell lines [22] as well as an antiviral agent against Vero cells infected with HSV-1 [23]. The second compound and its inositiol-related derivatives are common in biochemical pathways of plant-associated alpha-proteobacteria, where the influence of inositol catabolism on microbe-plant interactions has recently been described [24], however, it is unlikely that this simple sugar metabolite to have a role in QS inhibition, either. Some other linear molecules (glycerol, butanedioic acid, propanal, glycerol acetate, etc. )…”

Section: Resultsmentioning

confidence: 99%

Antibacterial and Anti-Quorum Sensing Molecular Composition Derived from Quercus cortex (Oak bark) Extract

Deryabin

Tolmacheva

2015

Molecules

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Abstract:Quercus cortex (Oak bark) has been used in European folk medicine since medieval times for treatment of diarrhea, stomatitis, pharyngitis and skin inflammations. Its antimicrobial activity is a well-known therapeutic property of oak bark, and its novel anti-quorum sensing (QS) ability has also been described recently. In this study, we examined the bioactive compounds of Quercus cortex extract and compared their direct antibacterial and regulatory anti-QS effects against Chromobacterium violaceum CV026 in a biotest. Evaluation of the original Quercus cortex extract showed weak antibacterial and prominent anti-QS activities that were retained and completely restored when the samples were dried and re-hydrated. The one-step liquid chromatography result indicated that the anti-QS activity might be determined by hydrophobic compounds; however, the subsequent reverse phase high performance liquid chromatography led to dissipation and loss of the activity. The gas chromatography-mass spectrometry gave excellent resolution between a majority of the compounds. Based on this result, 10 of the 35 identified small molecules were selected for further screening. The subsequent investigation indicated several compounds determined both the antibacterial and anti-QS activities of the Quercus cortex extract. Direct antibacterial activity was shown for 1,2,3-benzenetriol and 4-propyl-1,3-benzenediol, while sub-inhibitory concentrations of these compounds led to anti-QS effects. Five compounds: 4-(3-hydroxy-1-propenyl)-2-methoxy-phenol; 3,4,5-trimethoxyphenol; 4-hydroxy-3-methoxybenzaldehyde; 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one and 2H-1-benzopyran-2-one were characterized as QS inhibitors independent of any effect on bacterial growth. Biologically relevant concentrations of each single component showed weak activity only while reconstruction of OPEN ACCESSMolecules 2015, 20 17094 the small molecule composition derived from the Quercus cortex extract provided comparable complementary activity against C. violaceum CV026 in the biotest as the crude extract.

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Section: Resultsmentioning

confidence: 99%