Bacterial flora associated with diseased freshwater ornamental fish

Walczak, Natalia; Puk, Krzysztof; Guz, Leszek

doi:10.1515/jvetres-2017-0070

Cited by 60 publications

(40 citation statements)

References 25 publications

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“…Serratia, Edwardsiella, Plesiomonas, and Reyranella are genera belonging to the Enterobacteriaceae family and the Proteobacteria phylum. These isolated bacterial species are facultative pathogens for fish and humans and may be isolated from fish without apparent symptoms of the disease (Walczak et al, 2017). Serratia produces serrawettin that acts as a wetting agent to reduce the surface tension of the environment (Chan et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)

Méndez-Pérez

García-López

Bautista-López

et al. 2020

Lat. Am. J. Aquat. Res.

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Tropical gar (Atractosteus tropicus) is freshwater and estuarine fish, inhabiting the Earth since the Mesozoic era and undergoing limited physiological variation ever since. Besides its recognized cultural andscientific relevance, the species has seen remarkable growth in its economic impact due to pisciculture. In this study, we present the first report of the whole taxonomic composition of microbial communities in gut contents in juveniles and adults of A. tropicus, by sex and origin (wild and cultivated). For this study, 508 genera were identified, with the most and least abundant being Cetobacterium and Paludibacter, respectively. Fusobacteria, Proteobacteria, Firmicutes, and Bacteroidetes phyla are the core gut microbiome of A. tropicus juvenile and adult by sex and origin. Deinococcus-Thermus phylum sequence was only identified in wild-type males. In the phylogenetic trees reconstruction Lactococcus lactis strains CAU929 and CAU6600, Cp6 and CAU9951,Cetobacterium strain H69, Aeromonas hydrophila strain P5 and WR-5-3-2, Aeromonas sobria strain CP DC28 and Aeromonas hydrophila were identified, some of them with probiotic potential within the three dominant phyla in core gut microbiome in A. tropicus adults, especially in wild-type organisms. Myroides genus was recognized in microbiota gut of the cultivated juvenile A. tropicus. Nevertheless, Alpha diversity indicated that the highest gut microbiota abundance and richness is found in cultivated juvenile and wild-type adult A. tropicus female, rather than adult wild-type males and the least gut microbiota abundance and richness is found in a cultivated adult of A. tropicus for both sexes.

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Section: Discussionmentioning

confidence: 99%

High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)

Méndez-Pérez

García-López

Bautista-López

et al. 2020

Lat. Am. J. Aquat. Res.

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“…Other bacterial species from this study are known to be opportunistic pathogens in fish, some already described in S. aurata : C. freundii complex, Staphylococcus spp., K. pneumoniae , E. faecalis and E. cloacae [ 55 , 56 , 57 , 58 , 59 ]. All these species have pathogenic significance for humans, causing foodborne diseases, meningitides, wound and urinary tract infections, bacteremia, bone and joint infections, endocarditis, among others [ 59 , 60 , 61 , 62 , 63 ].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Diversity and Antibiotic Susceptibility of Sparus aurata from Aquaculture

et al. 2020

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In a world where the population continues to increase and the volume of fishing catches stagnates or even falls, the aquaculture sector has great growth potential. This study aimed to contribute to the depth of knowledge of the diversity of bacterial species found in Sparus aurata collected from a fish farm and to understand which profiles of diminished susceptibility to antibiotics would be found in these bacteria that might be disseminated in the environment. One hundred thirty-six bacterial strains were recovered from the S. aurata samples. These strains belonged to Bacillaceae, Bacillales Family XII. Incertae Sedis, Comamonadaceae, Enterobacteriaceae, Enterococcaceae, Erwiniaceae, Micrococcaceae, Pseudomonadaceae and Staphylococcaceae families. Enterobacter sp. was more frequently found in gills, intestine and skin groups than in muscle groups (p ≤ 0.01). Antibiotic susceptibility tests found that non-susceptibility to phenicols was significantly higher in gills, intestine and skin samples (45%) than in muscle samples (24%) (p ≤ 0.01) and was the most frequently found non-susceptibility in both groups of samples. The group of Enterobacteriaceae from muscles presented less decreased susceptibility to florfenicol (44%) than in the group of gills, intestine and skin samples (76%). We found decreased susceptibilities to β-lactams and glycopeptides in the Bacillaceae family, to quinolones and mupirocin in the Staphylococcaceae family, and mostly to β-lactams, phenicols and quinolones in the Enterobacteriaceae and Pseudomonadaceae families. Seven Enterobacter spp. and five Pseudomonas spp. strains showed non-susceptibility to ertapenem and meropenem, respectively, which is of concern because they are antibiotics used as a last resort in serious clinical infections. To our knowledge, this is the first description of species Exiguobacterium acetylicum, Klebsiella michiganensis, Lelliottia sp. and Pantoea vagans associated with S. aurata (excluding cases where these bacteria are used as probiotics) and of plasmid-mediated quinolone resistance qnrB19-producing Leclercia adecarboxylata strain. The non-synonymous G385T and C402A mutations at parC gene (within quinolone resistance-determining regions) were also identified in a Klebsiella pneumoniae, revealing decreased susceptibility to ciprofloxacin. In this study, we found not only bacteria from the natural microbiota of fish but also pathogenic bacteria associated with fish and humans. Several antibiotics for which decreased susceptibility was found here are integrated into the World Health Organization list of “critically important antimicrobials” and “highly important antimicrobials” for human medicine.

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“…Serratia, Edwardsiella, Plesiomonas and Reyranella are genera belonging to Enterobacteriaceae family and Proteobacteria phylum. This isolated bacterial species are facultative pathogens for fish and humans and may be isolated from fish without apparent symptoms of the disease (Walczak et al . 2017).…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiome Analysis In Adult Tropical Gars (Atractosteus tropicus)

Méndez-Pérez

García-López

Bautista-López

et al. 2019

Preprint

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Tropical gar (Atractosteus tropicus), is freshwater and estuarine fish that has inhabited 17 the Earth since the Mesozoic era, undergoing limited physiological variation ever since. This 18 omnivorous fish is endemic to southern Mexico and part of Central America. Besides its 19 recognized cultural and scientific relevance, the species has seen remarkable growth in its 20 economic impact due to pisciculture. Previous studies have highlighted the role of microbial 21 communities in fish, particularly those in the gut microbiome, in maintaining their host 22 homeostasis or disease. In this study, we present the first report of the whole taxonomic 23 composition of microbial communities in gut contents of adults' A. tropicus, by sex (female/male) 24 and origin (wild/cultivated). Using culture-independent techniques, we extracted metagenomic 25 DNA that was used for high throughput 16S rDNA profiling by amplifying the V4 -V5 26 hypervariable regions of the bacterial gene. A total of 364,735 total paired-end reads were 27 obtained on an Illumina MiSeq sequencing platform, belonging to 508 identified genera, with the 28 most and least abundant are Cetobacterium, Edwardsiella, Serratia, Clostridium sensu stricto, 29Paludibacter and Campylobacter, Snodgrassella, Albirhodobacter, Lentilitoribacter, respectively. We 30 detected that, by sex and origin, Proteobacteria, Fusobacteria, Firmicutes and Bacteroidetes phyla 31 are the core gut microbiome of the adults' A. tropicus. We discover the Deinococcus-Thermus 32 phylum sequence, wildtype males only, with extremophile capacity in another freshwater fish. 33We also identified the species Lactococcus lactis strains CAU929 and CAU6600, Cp6 and CAU9951, 34Cetobacterium strain H69, Aeromonas hydrophila strains P5 and WR-5-3-2, Aeromonas sobria strain 35 CP DC28 and Aeromonas hydrophila with probiotic potential in aquaculture within the three 36 dominant phyla, especially in wild-type organisms. 37

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Bacterial flora associated with diseased freshwater ornamental fish

Cited by 60 publications

References 25 publications

High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)

High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)

Bacterial Diversity and Antibiotic Susceptibility of Sparus aurata from Aquaculture

Gut Microbiome Analysis In Adult Tropical Gars (Atractosteus tropicus)

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