Bacterial communities regulate temporal variations of the antibiotic resistome in soil following manure amendment

Cheng, Jianhua; Tang, Xiangyu; Liu, Chen

doi:10.1007/s11356-021-12746-8

Cited by 9 publications

(3 citation statements)

References 49 publications

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“…It is common to quantify the contribution of biotic factors, such as taxonomy, MGEs, and their interaction, to ARG variation through a variation partitioning analysis (VPA). , Further, as mentioned above, in addition to taxonomy and MGEs, the function also has a strong and significant correlation with ARGs and may contribute to the ARG profile. In this work, VPA was first performed on the basis of taxonomy, MGEs, and ARGs, and then function (e.g.…”

Section: Results and Discussionmentioning

confidence: 99%

“…The results are consistent with previous findings that microbial phylogeny structures soil resistomes. 44,45 Next, with the contribution of taxonomy, MGEs, and their interaction to ARG variation as a reference, we analyze the effect of introduction of function on an explanation of ARG variation. For the introduction of CAZy (Figure S7a), taxonomy (17.70%) defines a much larger contribution in comparison to MGEs (7.70%), CAZy (6.90%), and their interaction (4.90%), while the unexplained ARG variation is as high as 63.00%, revealing the inhibition of CAZy in explaining ARG variation.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Metagenomic Insight into Sulfonamide-Induced Variation in Antibiotic Resistome of Soil Associated with Taxonomy, Mobile Genetic Elements (MGEs), and Function

Xiao

Jiang

et al. 2021

ACS Agric. Sci. Technol.

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Antibiotic resistance genes (ARGs) are emerging environmental pollutants, and the effect of both abiotic (e.g., anthropogenic pollutants such as sulfonamides (SAs)) and biotic (e.g., microbial community (taxonomy), mobile genetic elements (MGEs)) factors on the antibiotic resistome (collection of ARGs) has received much attention recently. In addition, function (e.g., CAZy, eggNOG and KEGG) is also an important biotic factor; however, how function correlates to ARGs and thus contributes to the antibiotic resistome remains ambiguous. This work explores the effect of sulfonamides (SAs) on ARGs and MGEs by a metagenomic analysis, a principal component analysis (PCA), and a nonmetric multidimensional scaling analysis (NMDS). Then, the work establishes a correlation between ARGs and function with a co-occurrence network and reveals the contribution and positive (negative)/direct (indirect) effect of taxonomy, MGEs, and function to the antibiotic resistome by variation partitioning analysis (VPA) and partial least-squares-path modeling (PLS-PM). The results show that the soil has diverse ARGs and several MGEs and SAs impose significant effects on them. Further, CAZy, eggNOG, and KEGG (including pathway and orthology) show strong and significant correlation with ARGs on the basis of the co-occurrence network; however, in our system it seems that only the KEGG pathway promotes the interpretation of ARGs and should be regarded as the priority function on considering the role of function in structuring the antibiotic resistome. This work reveals the importance and necessity of function as a biotic factor in shaping the antibiotic resistome in diverse resistome-related fields.

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Section: Results and Discussionmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Metagenomic Insight into Sulfonamide-Induced Variation in Antibiotic Resistome of Soil Associated with Taxonomy, Mobile Genetic Elements (MGEs), and Function

Xiao

Jiang

et al. 2021

ACS Agric. Sci. Technol.

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“…Among them, sul2 was signi cantly correlated with 8 bacterial genera except Pseudomonas (P < 0.001).Both tetA and tetR were carried by Aeromonas, Mucilaginibacter, Pseudomonas, Comamonas, Shewanella, Lactococcus and Algoriphagus. Different from tetA and tetR, the potential host bacteria of tetX were Algoriphagus, Lactococcus, Luteolibacter, Paenibacillus, Shewanella, Flavobacterium and Aeromonas.The large number of potential host bacteria of ARGs indicates that the evolution of bacterial community structure may be an important mechanism affecting the distribution of ARGs in the manure-amended soil-lettuce-snail food chain system.Chen 's results also suggest that soil microbes may be transferred to the leaf surface by some way, and the change of ARGs in the phyllosphere can be achieved by changing the phyllosphere microorganisms(CHEN Q-L et al,2018).The composition and distribution of bacterial communities at different interfaces such as soil, river and sludge are important factors affecting the dynamic changes of ARGs spectrum (TELMA F, et al,2019).These results suggest that bacterial community structure as another driving factor plays an important role in the spread of ARGs( CHENG J et al,2021).By studying the changes in the distribution of bacterial community composition and the host relationship with ARGs, the risk of ARGs transmission in the food chain can be further clari ed.…”

Section: Co-occurrence Of Args and Bacterial Community In Food Chain ...mentioning

confidence: 99%

Fecal antibiotic resistance genes were transferred through the distribution of soil-lettuce-snail food chain

Zhang

Zhao

Chen

et al. 2023

Environ Sci Pollut Res

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Massive antibiotic resistance genes (ARG) were detected in the soil modi ed by manure, which may affect human life safety through the food chain. However, the transmission of ARGs through the soil-plant-animal food chain is still unclear. Therefore, this study used high-throughput quantitative PCR technology to explore the effects of pig manure application on ARGs and bacterial communities in soil, lettuce phyllosphere and snail excrement.The results showed that a total of 384 ARGs and 48 MEGs were detected in all samples after 75 days of incubation. The diversity of ARGs and MGEs in soil components increased signi cantly by 87.04%and 40%with the addition of pig manure. The absolute abundance of ARGs in the phyllosphere of lettuce was signi cantly higher than that of the control group, with a growth rate of 212.5%.Six common ARGs were detected between the three components of the fertilization group, indicating that there was internal transmission of fecal ARGs between the trophic levels of the food chain.Firmicutes and Proteobacteria were identi ed as the dominant host bacteria in the food chain system, which were more likely to be used as carriers of ARGs to promote the spread of resistance in the food chain.The results were used to evaluate the potential ecological risks of livestock and poultry manure and provide theoretical basis and scienti c support for the formulation of ARGs prevention and control policies.

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Effect of chicken manure on soil microbial community diversity in poultry keeping areas

Minkina

Sushkova

Delegan

et al. 2022

Environ Geochem Health

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Bacterial communities regulate temporal variations of the antibiotic resistome in soil following manure amendment

Cited by 9 publications

References 49 publications

Metagenomic Insight into Sulfonamide-Induced Variation in Antibiotic Resistome of Soil Associated with Taxonomy, Mobile Genetic Elements (MGEs), and Function

Metagenomic Insight into Sulfonamide-Induced Variation in Antibiotic Resistome of Soil Associated with Taxonomy, Mobile Genetic Elements (MGEs), and Function

Fecal antibiotic resistance genes were transferred through the distribution of soil-lettuce-snail food chain

Effect of chicken manure on soil microbial community diversity in poultry keeping areas

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