Bacterial Communities Associated with the Biofilms Formed in High-Altitude Brackish Water Pangong Tso Located in the Himalayan Plateau

Chaudhari, Diptaraj; Dhotre, Dhiraj; Jani, Kunal; Sharma, Avinash; Singh, Yadvinder; Shouche, Yogesh S.; Rahi, Praveen

doi:10.1007/s00284-020-02244-4

Cited by 13 publications

(21 citation statements)

References 52 publications

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“…Signi cant differences recorded in the prokaryotic community structure of water and sediments of the Dal Lake suggested that the lake water is not a mere suspension of sediments as reported previously for the high-altitude Pangong Lake (Chaudhari et al 2020b). The higher abundance of prokaryotic genera including Geobacter, Thiobacillus, Desul tobacterium¸ Desulfatiglans, Thermoanaerobaculum, Thermincola, Ferruginibacter, Lutispora and Candidatus Nitrosotalea, which have been known for various biogeochemical processes, was noted in sediments of the Dal Lake.…”

supporting

confidence: 66%

Prokaryotic communities adapted to microhabitats on the Indian lotus (Nelumbo nucifera) growing in the high-altitude urban Dal Lake

et al. 2022

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Indian lotus (Nelumbo nucifera) is one of the dominant aquatic plants cultivated in Dal Lake, situated at 1586 m above MSL in the northeast of Srinagar, Kashmir. Despite their economic and ecological role, the microbial communities associated with the lotus plant are still unexplored. In this study, we investigated prokaryotic communities harboured by different lotus microhabitats (roots, rhizome, leaves, owers and fruits), lake water, and sediments using 16S rRNA gene amplicon sequencing. Overall, prokaryotic diversity decreased signi cantly on the lotus in comparison to the lake water and sediments. Among the microhabitats of lotus, roots and leaves harboured more diverse communities in comparison to rhizomes, fruits and owers. A total of 98 genera were shared by lotus and the Dal Lake sediments and water.However, signi cant differences were found in their relative abundance, like Pseudomonas was the most dominant genus on most lotus microhabitats. On the other hand, Flavobacterium was highly abundant in the lake water, while a higher abundance of Acinetobacter was recorded in sediments. Additionally, signi cant differences were recorded in the proportion of several genera including Pseudomonas, Clostridium, Sphingomonas, Erwinia, Lactococcus, Rhizobium, Weissella, and Raoultella among the lotus microhabitats. Predicted functions of prokaryotic communities revealed a higher abundance of genes associated with nutrient uptake in the microhabitats of the lotus. This study offered rst-hand information on the prokaryotic communities harboured by lotus plants and water and sediments of the Dal Lake and demonstrated the adaptation of diverse communities to microhabitats of lotus.

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confidence: 66%

Prokaryotic communities adapted to microhabitats on the Indian lotus (Nelumbo nucifera) growing in the high-altitude urban Dal Lake

et al. 2022

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“…The reconstructed summer salinity of lake-water varies from 4 to 6 ppt (Figure 9). Modern lake salinity and surface water temperature of the Pangong Tso are 8 ± 0.2 ppt and 14°C ± 1°C, respectively (Chaudhari et al, 2020). The reconstructed shell growth temperature (using equation (2)), that is, the water temperature at 0.5–2 m depth, varied from 5°C to 7°C with a mean value of 6°C (Figure 9).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, near the points of input from the streams the lake has a relatively high concentration of zooplankton such as red coloured copepods, Diaptomus sp. and their larvae (Bhat et al, 2011; Chaudhari et al, 2020). But the overall biodiversity of the Pangong Tso is limited due to its high salinity and extreme environmental conditions such as high temperature variability, high UV radiation, low nutrient level and buffering capacity.…”

Section: Study Area: Geomorphologic and Climatic Settingmentioning

confidence: 99%

“…in the littoral zone), suggesting the probable paleo-lake level (Martini et al, 2017) that if mapped and dated can provide important records of lake level fluctuations vis-à-vis climate change. Pangong Tso is a oligotrophic high-altitude lake (Chaudhari et al, 2020). The regional ISM rainfall pattern modifies the discharge of rivers as well as the volume of large lakes in the north-western Himalaya that apparently have broad correlations with the hydrological conditions of glaciers, glacial-lakes and northern hemisphere temperature regulation of the ISM and westerlies (Bookhagen and Burbank, 2010; Mehta et al, 2021; Sharma et al, 2016; Shukla et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Late-Holocene hydrological variability from the NW Himalaya and southwestern Tibetan Plateau: Paleo-salinity records from Pangong Tso

et al. 2023

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Variations in lake-levels and hydrology are connected to climatic dynamics over the Trans-Himalaya and Tibetan Plateau (TP). Pangong Tso, a ~140 km long hypersaline lake, is sensitive to changes in air temperature, precipitation and snow-glacier melt over the southwestern TP. The incised tributaries entering the lake expose deltaic sequences constituting topset, foreset and bottomset. Sedimentology, chronology and diatom analysis of delta sediments; and stable isotopic and sclerochronological analysis of gastropods helped delineate the Late-Holocene variation in lake levels, surface temperature and salinity. The elevation of the topsets is considered as representing past lake high-stands from where molluscs were also recovered. Three phased lake level changes are observed during the past 3 ka. The first high-stand (+1.4–3.0 m) was at ~2.8–2.0 ka when lake surface salinity and temperature were 4.67–6.01 ppt (parts per thousand) and 5°C–7°C, respectively, against the modern average values of 7.7 ± 0.09 ppt and 16.1°C ± 2.0°C. Followed by a brief decline, another high-stand (+3.0–3.6 m) is observed at 1.1 ka when the salinity is reduced to 4.03–5.72 ppt and lake surface temperature to 5°C–8°C. A corresponding increase in freshwater diatom concentration is also observed here. The third phase over the past 1 ka witnessed a fall of ~3.6–6 m in lake level that is attributed to an abrupt rise in aridity over the TP. We demonstrate that lake level variation in the region is a function of the variability of the Indian Summer Monsoon (ISM) and the westerlies, however during the high-stand, the hydrology of the lake was dominated by ISM precipitation.

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“…Microbial diversity analysis of the lake has been previously undertaken using both culturable and molecular approaches. Yadav et al [ 14 ] described the composition of psychrotrophic bacteria by using traditional cultivation-based approaches and identified the phylum Bacillota (genus Bacillus ) to be predominant with other reports targeting psychrotrophic bacteria in an array of biotechnological applications and their role in biogeochemical cycles [ 15 , 16 ] Culture-independent analysis has identified Pseudomonadota (genera Loktanella , Rhizobium , Marinobacter , Pseudomonas , Methylophaga ) as the dominant taxa followed by Bacteroidota (genus Gramella ) and Bacillota (genus Zunogwangia ) [ 16 , 17 , 18 ] and an analysis of a soil–water mix collected beneath frozen ice revealed the dominance of Bacteroidota followed by Bacillota and Pseudomonadota [ 19 ]. Further, in high-altitude lakes (Pangong Tso, Dashair, Gurudongmar, Chandratal, Dal) of the Indian trans-Himalayan region [ 20 ] and in other continents (Spain, Dry-Andes, Australian Alps, and California [ 21 , 22 , 23 , 24 ]), similar patterns of limited bacterial diversity have been reported.…”

Section: Introductionmentioning

confidence: 94%

Analysis of Culturable Bacterial Diversity of Pangong Tso Lake via a 16S rRNA Tag Sequencing Approach

Yadav,

Das,

Sundharam

et al. 2024

Microorganisms

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The Pangong Tso lake is a high-altitude freshwater habitat wherein the resident microbes experience unique selective pressures, i.e., high radiation, low nutrient content, desiccation, and temperature extremes. Our study attempts to analyze the diversity of culturable bacteria by applying a high-throughput amplicon sequencing approach based on long read technology to determine the spectrum of bacterial diversity supported by axenic media. The phyla Pseudomonadota, Bacteriodetes, and Actinomycetota were retrieved as the predominant taxa in both water and sediment samples. The genera Hydrogenophaga and Rheinheimera, Pseudomonas, Loktanella, Marinomonas, and Flavobacterium were abundantly present in the sediment and water samples, respectively. Low nutrient conditions supported the growth of taxa within the phyla Bacteriodetes, Actinomycetota, and Cyanobacteria and were biased towards the selection of Pseudomonas, Hydrogenophaga, Bacillus, and Enterococcus spp. Our study recommends that media formulations can be finalized after analyzing culturable diversity through a high-throughput sequencing effort to retrieve maximum species diversity targeting novel/relevant taxa.

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Bacterial Communities Associated with the Biofilms Formed in High-Altitude Brackish Water Pangong Tso Located in the Himalayan Plateau

Cited by 13 publications

References 52 publications

Prokaryotic communities adapted to microhabitats on the Indian lotus (Nelumbo nucifera) growing in the high-altitude urban Dal Lake

Prokaryotic communities adapted to microhabitats on the Indian lotus (Nelumbo nucifera) growing in the high-altitude urban Dal Lake

Late-Holocene hydrological variability from the NW Himalaya and southwestern Tibetan Plateau: Paleo-salinity records from Pangong Tso

Analysis of Culturable Bacterial Diversity of Pangong Tso Lake via a 16S rRNA Tag Sequencing Approach

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