Bacterial adhesion on biomedical surfaces covered by yttria stabilized zirconia

Pérez‐Tanoira, Ramón; Horwat, David; Kinnari, Teemu J.; Pérez‐Jorge, Concepción; Gómez‐Barrena, Enrique; Migot, Sylvie; Esteban, Jaime

doi:10.1007/s10856-015-5625-x

Cited by 8 publications

(6 citation statements)

References 31 publications

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“…6 The adherence and colonization of either bacterial cells or tissue cells on biomaterials have often been addressed as separate issues. [7][8][9][10] On the one hand, the outcome of the race for the surface between bacteria and tissue cells appears to be dependent on the initial number of bacteria present prior to cell seeding, as suggested by the fact that both the number of host cells and spread area per host cell decreases with increasing density of adhering bacteria. 11,12 However, the influence of the critical initial event of Additional Supporting Information may be found in the online version of this article.…”

Section: Introductionmentioning

confidence: 99%

Competitive colonization of prosthetic surfaces bystaphylococcus aureusand human cells

Pérez‐Tanoira

Han

Soininen

et al. 2016

J Biomedical Materials Res

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Implantation of a biomaterial provides an adhesion substratum both to host cell integration and to contaminating bacteria. We studied simultaneous competitive adhesion of Staphylococcus aureus in serial 1:10 dilutions of 10 colony forming units (CFU)/mL and human osteogenic sarcoma (SaOS-2) or primary osteoblast (hOB) cells, both 1x10 cells/mL, to the surfaces of titanium, polydimethylsiloxane and polystyrene. The bacterial adherence and human cell proliferation, cytotoxicity and production of reactive oxygen species (ROS) were studied using fluorometric (fluorescent microscopy and flow cytometry) and colorimetric methods (MTT, LDH and crystal violet). The bacterial cell viability was also evaluated using the drop plate method. The presence of bacteria resulted in reduced adherence of human cells to the surface of the biomaterials, increased production of ROS, and into increased apoptosis. On the other hand, the presence of either type of human cells was associated with a reduction of bacterial colonization of the biomaterial with Staphylococcus aureus. These results suggest that increasing colonization of the biomaterial surface in vitro by one negatively affects colonization by the other. Host cell integration to an implant surface reduces bacterial contamination, which opens novel opportunities for the design of infection-resistant biomaterials in current implantology and future regenerative medicine. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 62-72, 2017.

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Section: Introductionmentioning

confidence: 99%

Competitive colonization of prosthetic surfaces bystaphylococcus aureusand human cells

Pérez‐Tanoira

Han

Soininen

et al. 2016

J Biomedical Materials Res

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“…ZrO 2 and particularly YSZ nanoparticles present high ionic conductivity, mechanical strength, chemical inertness, high melting point, low thermal conductivity [50] as well as biocompatibility [51], characteristics that make zirconia nanoparticles attractive for a wide range of applications. Yttria-stabilized zirconia nanoparticles offer multiple advantages compared to pure ZrO 2 in terms of high mechanical properties, antibacterial properties and low thermal conductivity that make them attractive candidates for fillers in dental cement and composite materials [52,53].…”

Section: Discussionmentioning

confidence: 99%

Sol–Gel Synthesis and Characterization of YSZ Nanofillers for Dental Cements at Different Temperatures

et al. 2021

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Background: Yttria-stabilized zirconia nanoparticles can be applied as fillers to improve the mechanical and antibacterial properties of luting cement. The aim of this study was to synthesize yttria-stabilized zirconia nanoparticles by the sol–gel method and to investigate their composition, structure, morphology and biological properties. Methods: Nanopowders of ZrO2 7 wt% Y2O3 (nY-ZrO) were synthesized by the sol–gel method and were sintered at three different temperatures: 800, 1000 and 1200 °C, and their composition, size and morphology were investigated. The biocompatibility was investigated with human gingival fibroblasts (hGFs), while reactive oxygen species (ROS) production was evaluated through fluorescence analysis. Results: All synthesized materials were composed of tetragonal zirconia, while nanopowders sintered at 800 °C and 1000 °C additionally contained 5 and 20 wt% of the cubic phase. By increasing the calcination temperature, the crystalline size of the nanoparticles increased from 12.1 nm for nY-ZrO800 to 47.2 nm for nY-ZrO1200. Nano-sized particles with good dispersion and low agglomeration were received. Cell culture studies with human gingival fibroblasts verified the nanopowders’ biocompatibility and their ROS scavenging activity. Conclusions: the obtained sol–gel derived nanopowders showed suitable properties to be potentially used as nanofillers for dental luting cement.

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“…Similarly, processes aimed at nanotexturing ZrO 2 revealed a reduction in bacterial adhesion [ 107 ] ( Table 2 ). In addition, authors reported [ 108 ] the development of a surface based on Ti-coated alloy with a combination of cubic stabilized zirconia and silver films, significantly reducing the adhesion of bacteria such as Staphylococcus aureus and Staphylococcus epidermidis which are responsible for peri-implant pathology.…”

Section: Biological Responsesmentioning

confidence: 99%

Oral Tissue Interactions and Cellular Response to Zirconia Implant-Prosthetic Components: A Critical Review

et al. 2021

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Background: Dental components manufactured with zirconia (ZrO2) represent a significant percentage of the implant prosthetic market in dentistry. However, during the last few years, we have observed robust clinical and pre-clinical scientific investigations on zirconia both as a prosthetic and an implantable material. At the same time, we have witnessed consistent technical and manufacturing updates with regards to the applications of zirconia which appear to gradually clarify points which until recently were not well understood. Methods: This critical review evaluated the “state of the art” in relation to applications of this biomaterial in dental components and its interactions with oral tissues. Results: The physico-chemical and structural properties as well as the current surface treatment methodologies for ZrO2 were explored. A critical investigation of the cellular response to this biomaterial was completed and the clinical implications discussed. Finally, surface treatments of ZrO2 demonstrate that excellent osseointegration is possible and provide encouraging prospects for rapid bone adhesion. Furthermore, sophisticated surface treatment techniques and technologies are providing impressive oral soft tissue cell responses thus leading to superior biological seal. Conclusions: Dental devices manufactured from ZrO2 are structurally and chemically stable with biocompatibility levels allowing for safe and long-term function in the oral environment.

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Bacterial adhesion on biomedical surfaces covered by yttria stabilized zirconia

Cited by 8 publications

References 31 publications

Competitive colonization of prosthetic surfaces bystaphylococcus aureusand human cells

Competitive colonization of prosthetic surfaces bystaphylococcus aureusand human cells

Sol–Gel Synthesis and Characterization of YSZ Nanofillers for Dental Cements at Different Temperatures

Oral Tissue Interactions and Cellular Response to Zirconia Implant-Prosthetic Components: A Critical Review

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