Bacteria isolated on TCBS media associated with hatched Artemia cysts of commercial brands

Phaeobacter inhibens as probiotic bacteria in non-axenic Artemia and algae cultures

“…This may potentially be a problem when supplying probionts through the live feed as different kinds of live feed already have different established microbiotas (Austin and Allen, 1982;Berland et al, 1970;Grossart and Simon, 2007;López-Torres and Lizárraga-Partida, 2001;Nakase and Eguchi, 2007;Salvesen et al, 2000;Van Stappen, 1996). P. inhibens have previously been shown to colonize axenic algae (Seyedsayamdost et al, 2011b(Seyedsayamdost et al, , 2014 as well as other live feed subjects such as Artemia and rotifers (D'Alvise et al, 2012;Grotkjaer et al, 2016), but our data suggest that this is also the case when P. inhibens is co-cultured with an existing microbiota.…”

“…The nauplii of the brine shrimp Artemia are the most commonly used live feed organism in aquaculture industry (Van Stappen, 1996). Unfortunately the Artemia nauplii are also a vector for introducing bacteria into the hatchery systems (Austin and Allen, 1982;Benavete and Gatesoupe, 1988) herein opportunistic pathogenic bacteria (Gomez-Gil et al, 1994;López-Torres and Lizárraga-Partida, 2001). The bacterial community of newly hatched nauplii is dominated by uncultured members of Gammaproteobacteria and Planctomycetales situated in the gut rather than at their external surfaces (Høj et al, 2009), and can reach densities of up to 10 7 within 24 h (Austin and Allen, 1982).…”

Proliferation, colonization, and detrimental effects of Vibrio parahaemolyticus and Vibrio harveyi during brine shrimp hatching

“…The ability of brine shrimp nauplii to accumulate bacteria has been broadly documented (Gómez-Gil et al, 2004;López-Torres and Lizárraga-Partida, 2001;Soto-Rodriguez et al, 2003). This attribute has been used to produce biocapsule and to induce experimental vibriosis in fish and shellfish larvae.…”

“…is a main vector of microbes for cultured organisms, i.e., cysts are carriers of vibrios (Austin and Allen, 1982;López-Torres and Lizárraga-Partida, 2001), pathogenic yeasts (Moore and Strom, 2003) and viruses (Chang et al, 2002). Most of the cyst-associated microbes are acquired at harvest sites and can change with time (harvest season) and during harvesting processes (Straub and Dixon, 1993).…”

Phenotypic and genetic diversity ofVibrio alginolyticus strains recovered from juveniles and olderSparus aurata reared in a Tunisian marine farm

“…strains (Overman et al, 1985;Elliot et al, 1995). Several studies have demonstrated that these miniaturized biochemical tests, especially API 20 E and 20 NE were used with success to identify bacteria belonging to Vibrionaceae family isolated from different environmental biotopes (Croci et al, 2001, López-Torres and Lizárraga-Partida, 2001, O'Hara et al, 2003. Good identification of large environmental Vibrio strains was obtained when applying the biochemical keys proposed by Blanch (1994a, 1994b) and the same scheme modified by Ottaviani et al (2003).…”