Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests

Sauka, Diego Herman; Peralta, Cecilia; Pérez, Maximiliano; Onco, Maria Ines; Fiodor, Angelika; Caballero, Javier; Caballero, Primitivo; Berry, Colin; Valle, Eleodoro E. Del; Palma, Leopoldo

doi:10.1016/j.biocontrol.2022.104838

Cited by 8 publications

(7 citation statements)

References 43 publications

(55 reference statements)

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“…Moreover, it showed many antimicrobial properties [61] and targeted activity against B. cereus and Listeria monocytogenes through its toyoncin production ability [20]. A soil-recovered strain of B. toyonensis has also shown promising insecticidal activities in former studies [62].…”

Section: Discussionmentioning

confidence: 99%

Identification, Characterization, and Production Optimization of 6-Methoxy-1H-Indole-2-Carboxylic Acid Antifungal Metabolite Produced by Bacillus toyonensis Isolate OQ071612

El-Sayed,

Abdelaziz,

Ali

et al. 2023

Microorganisms

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Fungal infections currently pose a real threat to human lives. In the current study, soil bacterial isolates were screened for the production of antifungal compounds to combat human fungal pathogens. Notably, the bacterial F1 isolate exhibited antimycotic action towards the Candida albicans ATCC 10231 and Aspergillus niger clinical isolates. By employing phenotypic and molecular techniques, we identified the F1 isolate as the Bacillus toyonensis isolate OQ071612. The purified extract showed stability within a pH range of 6–7 and at temperatures of up to 50 °C. It demonstrated potential antifungal activity in the presence of various surfactants, detergents, and enzymes. The purified extract was identified as 6-methoxy-1H-Indole-2-carboxylic acid using advanced spectroscopic techniques. To optimize the antifungal metabolite production, we utilized response surface methodology (RSM) with a face-centered central composite design, considering nutritional and environmental variables. The optimal conditions were as follows: starch (5 g/L), peptone (5 g/L), agitation rate of 150 rpm, pH 6, and 40 °C temperature. A confirmatory experiment validated the accuracy of the optimization process, resulting in an approximately 3.49-fold increase in production. This is the first documented report on the production and characterization of 6-methoxy-1H-Indole-2-carboxylic acid (MICA) antifungal metabolite from Bacillus toyonensis.

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Section: Discussionmentioning

confidence: 99%

Identification, Characterization, and Production Optimization of 6-Methoxy-1H-Indole-2-Carboxylic Acid Antifungal Metabolite Produced by Bacillus toyonensis Isolate OQ071612

El-Sayed,

Abdelaziz,

Ali

et al. 2023

Microorganisms

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“…SDS-PAGE gel analysis revealed no detectable protein band for Mpp60Ba3. Sauka et al [65] discovered an mpp homolog in a B. toyonensis strain's genome but no protein bands by SDS-PAGE analysis, implying that these proteins are secreted and present in remnant fractions. The expression of Mpp60Aa and Mpp60Ba proteins has been detected in Bti AM65-52 [19] and Bt jegathesan [58] in low abundance.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Genomic, and Proteomic Characterization of a Novel Neotropical Strain of Bacillus thuringiensis with Mosquitocidal Activities

et al. 2023

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The combination of genomic and proteomic analyses is a useful tool for the study of novel Bacillus thuringiensis (Bt) strains, as these approaches allow the accurate identification of pesticidal proteins and virulence factors produced. Here, we isolated and evaluated the potential of a novel Neotropical Bt strain (TOD651) for controlling larvae of Aedes aegypti and Culex quinquefasciatus mosquitoes. Aiming for the full comprehension of the TOD651 larvicidal potential, we further evaluated the whole TOD651 genome and conducted the proteomic analysis of the TOD651 spore–crystal mixtures. Our results showed that Bt TOD651 similarly killed both A. aegypti (0.011 µg/mL) and C. quinquefasciatus (0.023 µg/mL) larvae, exhibiting similar potency to the commercial Bt strain. The genome sequence revealed that Bt TOD651 harbors cry11Aa3, cry10Aa4, cry4Aa4, cry4Ba5, cyt1Aa5, cyt1Ca1, cyt2Ba13, mpp60Aa3, and mpp60Ba3. The proteomic analysis revealed no expression of Mpp60Aa3, while all the other pesticidal proteins were expressed (Cry4Ba5 was more abundant than Cyt1Aa5). The expression of the Mppe showed the major proportions between proteases. The virulent factor neutral protease B and spore coat proteins were also expressed. The expression of relevant pesticidal proteins (e.g., Cry, Cyt, Mpp, and other pathogenic factors), whose actions can occur in a synergic relation, indicates that the biocontrol using Bt TOD651 may contribute to delaying the selection of resistant individuals.

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“…Although the Cry proteins with which Bt crops have been nuclearly armed have shown excellent performance in controlling different pests, the expression of lectins and other proteins with insecticidal capacity from Bacillus toyonensis and Lysinibacillus sphaericus has also been shown to be effective in controlling pests such as Alphitobius diaperinus, Spodoptera exigua, Cydia pomonella, Anthonomus grandis, Aedes aegypti, and Myzus persicae [131,132]. Furthermore, while the expression of these proteins in chloroplasts has not been thoroughly investigated, it is important to note them because despite the efficacy of Bt crops in controlling Lepidoptera and Coleoptera, control of Hemiptera has not been entirely successful, as many Hemipteran species have now become significant pests of Bt crops [133,134].…”

Section: Dunaliellaceaementioning

confidence: 99%

Chloroplasts: The Future of Large-Scale Protein Production

Chávez¹,

Ornelas²,

Cruz³

et al. 2024

Physiology

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Chloroplast engineering has matured considerably in recent years. It is emerging as a promising tool to address the challenges related to food security, drug production, and sustainable energy posed by an ever-growing world population. Chloroplasts have proven their potential by efficiently expressing transgenes, encapsulating recombinant proteins, and protecting them from cellular machinery, making it possible to obtain highly functional proteins. This quality has also been exploited by interfering RNA technology. In addition to the practical attributes offered by chloroplast transformation, such as the elimination of position effects, polycistronic expression, and massive protein production, the technique represents an advance in biosafety terms; however, even if its great biotechnological potential, crops that have efficiently transformed are still a proof of concept. Despite efforts, other essential crops have remained recalcitrant to chloroplast transformation, which has limited their expansion. In this chapter, we address the most recent advances in this area and the challenges that must be solved to extend the transformation to other crops and become the de facto tool in plant biotechnology.

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Bacillus toyonensis biovar Thuringiensis: A novel entomopathogen with insecticidal activity against lepidopteran and coleopteran pests

Cited by 8 publications

References 43 publications

Identification, Characterization, and Production Optimization of 6-Methoxy-1H-Indole-2-Carboxylic Acid Antifungal Metabolite Produced by Bacillus toyonensis Isolate OQ071612

Identification, Characterization, and Production Optimization of 6-Methoxy-1H-Indole-2-Carboxylic Acid Antifungal Metabolite Produced by Bacillus toyonensis Isolate OQ071612

Isolation, Genomic, and Proteomic Characterization of a Novel Neotropical Strain of Bacillus thuringiensis with Mosquitocidal Activities

Chloroplasts: The Future of Large-Scale Protein Production

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