<i>Bacillus thuringiensis</i> insecticidal delta‐endotoxin: Diversity of crystal proteins and its relatedness to the toxicity spectrum

Haider, Muhammad Zulqurnain; Mahmood, S.

doi:10.1002/jobm.3620300406

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…The molecular weights (kDa) of the major components of the crystal proteins of the standards in our experiment were 130-134 and 65-68 for HD-1 kurstaki, 128-135 and 65-27 for HD500 israelensis, and 130-135 and 60-66 for HD-133 aizawai and were similar to values reported for these varieties by other authors (Chak and Ellar 1987;Carlton 1988;Hofte and Whiteley 1989;Haider and Mahmood 1990). The two major bands seen in HD-1 are identical to those in our kurstaki isolate, A1.2172, which carries two distinct morphologies of protein crystals, a bipyramidal and a cuboidal (Fig.…”

Section: Characterization Of Selected Isolatessupporting

confidence: 90%

ISOLATION, CHARACTERIZATION, AND CULTURE OFBACILLUS THURINGIENSISFROM SOIL AND DUST FROM GRAIN STORAGE BINS AND THEIR TOXICITY FORMAMESTRA CONFIGURATA(LEPIDOPTERA: NOCTUIDAE)

et al. 1998

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One hundred and twoBacillus thuringiensisBerliner strains isolated from six different types of Canadian soil and dust from different grain storage bins were cultured in shake flasks containing Great Northern White Bean (GNWB) protein concentrate (48.6% protein) as the main nitrogen source and dextrose as the main carbohydrate source. The resulting endotoxins were bioassayed against the bertha armyworm,Mamestra configurataWlk. Thirty-three percent of soil and 66% of grain dust samples were positive forB. thuringiensis. The bacterium was found most frequently in organic-rich soil. The four most toxic soil isolates (which were seven to 15 times more toxic than the international standard, HD1-S-1980), and two nontoxic grain dust isolates were characterized by serological typing, biochemical analysis, carbohydrate utilization, plasmid profile analysis, protein profile analysis using sodium dodecyl sulfate – polyacrylamide gels, and polymerase chain reaction. Four isolates were determined to be subsp.kurstakicontaining 130–140 and 63–65 kDa proteins, and two isolates (tested for comparison) were subsp.canadensiscontaining 31 and 38 kDa proteins. Nonpyramidal-crystal-producing strains did not grow well in culture media containing GNWB, degossypellized cotton seed meal (61% protein), defatted soy flour (55% protein), or peptone as nitrogen sources. Excess of GNWB protein concentrate in shake flask culture media (30 g/L) inhibited bacterial growth and reduced the toxicity of isolate A1.2/72 subsp.kurstaki, which was the most toxic soil isolate. Isolate A1.2/72, which was 15 times more toxic for bertha armyworm larvae than the international standard (HD1-S-1980), contained threecry1Agenes (cry1Aa,cry1Ab, and cry1Ac), whereas HD-1 lacked thecry1Abgene. This strain was synergistic with strain HD-551 subsp.kenyae(cry1A,cry2A, andcry1Bgenes) but not with HD-133 subsp.aizawai(cry1Ab,cry1B,cry1C, andcry1Dgenes) when the strains were cultured together in a cotton seed meal medium and fed toM. configurata. The growth rate, economic yield, and toxicity of the new isolate, A1.2/72, produced in a 14-L laboratory fermenter declined when the fermentation ingredients were tripled. We believe that the indigenous strain A1.2/72 warrants further research development for bertha armyworm control.

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Section: Characterization Of Selected Isolatessupporting

confidence: 90%

ISOLATION, CHARACTERIZATION, AND CULTURE OFBACILLUS THURINGIENSISFROM SOIL AND DUST FROM GRAIN STORAGE BINS AND THEIR TOXICITY FORMAMESTRA CONFIGURATA(LEPIDOPTERA: NOCTUIDAE)

et al. 1998

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Section: Screening Of New Isolates Against Other Insect Speciessupporting

confidence: 90%

Effect of Pheromone Permeation on Sustained Flight of Male Spruce Budworm

Sanders

1998

Can Entomol

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“…The relation between the potential activity of the isolates and their protein pattern was investigated by many authors e.g. [10], [18], [34], [35], [36], [37].…”

Section: Discussionmentioning

confidence: 99%

Diversity of Bacillus thuringiensis isolates from Egyptian soils as shown by molecular characterization

Salama¹,

El-Ghany²,

Sakr³

2015

Journal of Genetic Engineering and Biotechnology

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Different techniques were adopted for molecular characterization of several indigenous strains of Bacillus thuringiensis (Bt) previously isolated from Egyptian soil samples. These isolates show different toxicity levels against neonate larvae of both insect species; Spodoptera littoralis (Biosduval); and Helicoverpa armigera (Hübner). The parasporal crystals among the most potent isolates contained polypeptides of about 127 and 130 kDa. PCR screening for genes encoding different Cry genes was performed. The Cry 1 gene is the most abundant in these isolates (83.33%) among tested Cry-type genes, followed by Cry 1 gene subfamilies (Cry 1B and Cry 1C) with percentage of 38.88% and 77.77%, respectively. The tested isolates showed the presence of Cry 2A(a,b) gene, but not all of these isolates were positive for Cry 2 gene (55.55%). Only 27.77% and 16.66% of the tested isolates harbor Cry 4 and Cry 3 genes, respectively. All strains were negative in PCR assays for the Vip 3Aa1 gene. Moreover, DNA fingerprinting using RAPD-PCR was performed to detect the genetic similarities and dissimilarities among the different isolates and standard strains. Assessment of Bt diversity based on the combined analysis of their protein and RAPD-PCR banding patterns was performed. This study demonstrates that Bt strains isolated from Egyptian soil samples can be distinguished and identified on the basis of the distribution of Cry-type genes and RAPD fingerprints.

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Bacillus thuringiensis insecticidal delta‐endotoxin: Diversity of crystal proteins and its relatedness to the toxicity spectrum

Cited by 5 publications

References 15 publications

ISOLATION, CHARACTERIZATION, AND CULTURE OFBACILLUS THURINGIENSISFROM SOIL AND DUST FROM GRAIN STORAGE BINS AND THEIR TOXICITY FORMAMESTRA CONFIGURATA(LEPIDOPTERA: NOCTUIDAE)

ISOLATION, CHARACTERIZATION, AND CULTURE OFBACILLUS THURINGIENSISFROM SOIL AND DUST FROM GRAIN STORAGE BINS AND THEIR TOXICITY FORMAMESTRA CONFIGURATA(LEPIDOPTERA: NOCTUIDAE)

Effect of Pheromone Permeation on Sustained Flight of Male Spruce Budworm

Diversity of Bacillus thuringiensis isolates from Egyptian soils as shown by molecular characterization

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