Bacillus mojavensis: biofilm formation and biochemical investigation of its bioactive metabolites

Camele, Ippolito; Elshafie, Hazem S.; Caputo, Lucia; Sakr, Shimaa H.; Feo, Vincenzo De

doi:10.4081/jbr.2019.8296

Cited by 42 publications

(39 citation statements)

References 43 publications

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“…Bacterial biofilm formation is a serious and ongoing concern in the food industry, mostly due to the ability of biofilm inhabitants to withstand adverse conditions [1,2]. The biofilm formation process is divided into separate stages and includes an attachment step of single cells to a surface, followed by microcolony formation and subsequent growth into mature biofilms with self-production of extracellular polymeric substances (EPS) [3].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Eugenol, Trans-Cinnamaldehyde, Citronellol, and Terpineol on Escherichia coli Biofilm Control as Assessed by Culture-Dependent and -Independent Methods

2020

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Bacterial biofilms contribute to problems with preserving food hygiene, jeopardizing any conventional intervention method used by the food industry. Hence, the approach of using essential oil (EO) compounds effective in biofilm control has considerable merit and deserves in-depth research. In this study, the effect of selected EO compounds (eugenol, trans-cinnamaldehyde, citronellol, and terpineol) was assessed on Escherichia coli biofilm control by plate count, resazurin assay, and Syto® 9/PI (-/propidium iodide) staining coupled with flow cytometry (FCM) and confocal laser scanning microscopy (CLSM). The selected EO compounds effectively inhibited the growth of planktonic E. coli at low concentrations of 3–5 mM, revealing a high antimicrobial activity. EO compounds markedly interfered with biofilms too, with trans-cinnamaldehyde causing the most prominent effects. Its antibiofilm activity was manifested by a high reduction of cell metabolic activity (>60%) and almost complete reduction in biofilm cell culturability. In addition, almost 90% of the total cells had perturbed cell membranes. Trans-cinnamaldehyde further impacted the cell morphology resulting in the filamentation and, thus, in the creation of a mesh network of cells. Citronellol scored the second in terms of the severity of the observed effects. However, most of all, it strongly prevented native microcolony formation. Eugenol and terpineol also affected the formation of a typical biofilm structure; however, small cell aggregates were still repeatedly found. Overall, eugenol caused the mildest impairment of cell membranes where 50% of the total cells showed the Syto® 9+/PI– pattern coupled with healthy cells and another 48% with injured cells (the Syto® 9+/PI+). For terpineol, despite a similar percentage of healthy cells, another 45% was shared between moderately (Syto® 9+PI+) and heavily (Syto® 9–PI+) damaged cells. The results highlight the importance of a multi-method approach for an accurate assessment of EO compounds’ action against biofilms and may help develop better strategies for their effective use in the food industry.

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Section: Introductionmentioning

confidence: 99%

The Effects of Eugenol, Trans-Cinnamaldehyde, Citronellol, and Terpineol on Escherichia coli Biofilm Control as Assessed by Culture-Dependent and -Independent Methods

2020

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“…The N. AHLs-deficient mutant (ICMP11096 glad -I) and the complemented one (ICMP11096 glad -IR) were evaluated for their ability to inhibit the growth of B. megaterium and E. coli in dual agar plate assay following the method of Lavermicocca et al (1997) as reported by Camele et al (2019). In particular, small masses of fresh bacterial cultures were transferred in the center of 90 mm Ø MMA Petri dishes.…”

Section: Methodsmentioning

confidence: 99%

Study of the Regulatory Role of N-Acyl Homoserine Lactones Mediated Quorum Sensing in the Biological Activity of Burkholderia gladioli pv. agaricicola Causing Soft Rot of Agaricus spp.

et al. 2019

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Many Burkholderia spp. produce in vitro secondary metabolites with relevant biological activities and potential practical applications. Burkholderia gladioli pv. agaricicola (Bga) possess promising biological activities regulated by N-Acyl homoserine lactones (N.AHLs) based quorum sensing (QS) mechanism. In the current study, N.AHLs-deficient (ICMP11096glad-I) and N.AHLs-complemented (ICMP11096glad-IR) mutants were constructed in which the gene coding for AHL synthase was inactivated by allelic exchange in glad I mutant strain. The aims of this research were to (i) assess the antagonistic activity of the wild type (WT) and the glad-I mutant of Bga against Bacillus megaterium (G+ve) and Escherichia coli (G−ve), (ii) screen their hydrolytic enzymes and hemolytic substances, (iii) monitor the pathogenic effect against Agaricus bisporus, and finally (iv) analyze the bioactive secondary metabolites produced by WT and mutant strain using high performance liquid chromatography (HPLC). Results showed that N.AHLs-deficient mutant exhibited high reduction of antagonistic activity against the tested microorganisms and notable reduction of chitinolytic, proteolytic and glucanolytic activities and complete absence of hemolytic activity, and the glad-IR complemented mutant was able to regain the major part of these activities. Furthermore, N.AHLs-deficient mutant strain was unable to degrade flesh cubes pseudo-tissues of A. bisporus. On the other hand, the virulence effect of complemented mutant was like to the parental WT strain. HPLC analysis revealed that some of the single components produced by WT strain were absent in N.AHLs-deficient mutant and others were highly reduced. The out-findings of the current research gave a spot into the regulatory role of N.AHLs and QS phenomenon in the biological activity of Bga bacterium.

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“…Microbial secondary metabolites (MSM) are the most promising source of novel natural products; hence their discovery and characterization are the objective of many researches for controlling important phyto- and human pathogens [ 53 , 54 , 55 ].…”

Section: Microbial Secondary Metabolitesmentioning

confidence: 99%

An Overview of Metabolic Activity, Beneficial and Pathogenic Aspects of Burkholderia Spp.

Elshafie

Camele

2021

Metabolites

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Burkholderia is an important bacterial species which has different beneficial effects, such as promoting the plant growth, including rhizosphere competence for the secretion of allelochemicals, production of antibiotics, and siderophores. In addition, most of Burkholderia species have demonstrated promising biocontrol action against different phytopathogens for diverse crops. In particular, Burkholderia demonstrates significant biotechnological potential as a source of novel antibiotics and bioactive secondary metabolites. The current review is concerned with Burkholderia spp. covering the following aspects: discovering, classification, distribution, plant growth promoting effect, and antimicrobial activity of different species of Burkholderia, shedding light on the most important secondary metabolites, their pathogenic effects, and biochemical characterization of some important species of Burkholderia, such as B. cepacia, B. andropogonis, B. plantarii, B. rhizoxinica, B. glumae, B. caryophylli and B. gladioli.

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Bacillus mojavensis: biofilm formation and biochemical investigation of its bioactive metabolites

Cited by 42 publications

References 43 publications

The Effects of Eugenol, Trans-Cinnamaldehyde, Citronellol, and Terpineol on Escherichia coli Biofilm Control as Assessed by Culture-Dependent and -Independent Methods

The Effects of Eugenol, Trans-Cinnamaldehyde, Citronellol, and Terpineol on Escherichia coli Biofilm Control as Assessed by Culture-Dependent and -Independent Methods

Study of the Regulatory Role of N-Acyl Homoserine Lactones Mediated Quorum Sensing in the Biological Activity of Burkholderia gladioli pv. agaricicola Causing Soft Rot of Agaricus spp.

An Overview of Metabolic Activity, Beneficial and Pathogenic Aspects of Burkholderia Spp.

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