Bacillus coagulans R11 maintained intestinal villus health and decreased intestinal injury in lead-exposed mice by regulating the intestinal microbiota and influenced the function of faecal microRNAs

Xing, Si-Cheng; Huang, Chun-Bo; Mi, Jiandui; Wu, Yinbao; Liao, Xindi

doi:10.1016/j.envpol.2019.113139

Cited by 35 publications

(24 citation statements)

References 40 publications

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“…In addition, Alistipes indistinctus existed in only the B. coagulans R11-fed groups. As previously mentioned, the abundance of the potential pathogens E. coli, Pseudomonas aeruginosa, and Salmonella was decreased after feeding laying hens exposed to lead B. coagulans R11 (Xing et al, 2019a). In the present study, we also found that the abundances of the potential pathogens Parabacteroides sp.…”

Section: Analysis Of the Cecum Microbiota Structure And Potential Patsupporting

confidence: 86%

“…A breeding experiment was mentioned in a previous study (Xing et al, 2019a), briefly, the 28-week-old Roman Pink laying hens used in this experiment and the weights were approximately 1.68 kg/bird. A total of 60 birds were separated into four treatment groups.…”

Section: Breeding Experimentsmentioning

confidence: 99%

“…There was 1 week before the normal experiment for laying hens to adapt and then 4 weeks for the normal experiment. The details of the breeding experiment were described in a previous study (Xing et al, 2019a).…”

Section: Breeding Experimentsmentioning

confidence: 99%

“…Expression of the atoB protein was upregulated in both Pb exposure groups, but the Kal protein expression was downregulated. Based on the results for group R11, these effects could be due to lead exposure enhancing all physiological metabolism to resist lead toxin stress, and the requirement of energy thus increased (Xing et al, 2019a). Acetyl-CoA is also an important substrate of the citrate cycle (Garland and Randle, 1964;Shepherd and Garland, 1969); thus, the synthesis of acetyl-CoA increased to increase energy production through the citrate cycle.…”

Section: α-Solanine Was a Major Alkaloid Metabolite In The B Coagulamentioning

confidence: 99%

“…Lead is a toxic heavy metal that has been made widely present in the environment through anthropic activities, especially in developing countries (Florea et al, 2005;Zoghi et al, 2014). Oral lead toxins damage the intestinal villi, which may increase their permeability and the possibility of pathogen invasion (Xing et al, 2019a). Probiotics absorb lead ions and decrease their number in the gut, and several studies have reported the effectiveness of different probiotics in heavy metal ion adsorption in animals (Tian et al, 2012;Zhai et al, 2013Zhai et al, , 2017.…”

Section: Introductionmentioning

confidence: 99%

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The Combination of Lead and Bacillus coagulans R11 Increased the Concentration of Alpha-Solanine in the Cecum of Laying Hens and the Pathogens Abundance Decreased

Xing¹,

Chen²,

Chen³

et al. 2020

Front. Microbiol.

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Alpha-solanine is an alkaloid that can inhibit the growth of pathogens and cancer cells, the present study proved that feeding with Bacillus coagulans R11 increases the concentration of alpha-solanine in the cecum of laying hens, which also decreases the abundance of potential pathogens. In addition, the bacteria genera, metabolism pathways and its proteins involved in the biosynthesis of alpha-solanine in the cecum were also characterized. The results showed that B. coagulans R11 feeding could increase the concentration of alpha-solanine, even with lead exposure. Mevalonic acid and MEP/DOXP pathways were both participated in the biosynthesis of alpha-solanine; at the same time, the gut metabolites (S)-2-amino-6-oxohexanoate, N2-succinyl-Lornithine and the bacteria proteins atoB, ispH were shown to be crucial role in the biosynthesis of alpha-solanine in the gut. The genera Faecalibacterium sp. An77 and Faecalibacterium sp. An58 2 were important in the biosynthesis of alpha-solanine, which provided the key proteins atoB and ispH. In addition, alpha-solanine could decrease the abundance of Prevotella sp. 109 and Prevotella marshii. In conclusion, alpha-solanine could be biosynthesized by cecal microorganisms with the stimulation of B. coagulans R11 in the intestine of laying hens, in addition, alpha-solanine was the main compound which also decreased the abundance of gut potential.

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Section: Analysis Of the Cecum Microbiota Structure And Potential Patsupporting

confidence: 86%

Section: Breeding Experimentsmentioning

confidence: 99%

Section: Breeding Experimentsmentioning

confidence: 99%

Section: α-Solanine Was a Major Alkaloid Metabolite In The B Coagulamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Combination of Lead and Bacillus coagulans R11 Increased the Concentration of Alpha-Solanine in the Cecum of Laying Hens and the Pathogens Abundance Decreased

Xing¹,

Chen²,

Chen³

et al. 2020

Front. Microbiol.

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Lead exposure results in defective behavior as well as alteration of gut microbiota composition in flies and their offsprings

Sun

Tang

et al. 2020

Intl J of Devlp Neuroscience

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Background: Lead (Pb) has become one of the most dangerous metals to human health, especially to the nervous system as its persistent accumulation and high toxicity. However, how the gut microbiota influence the Pb-related neurotoxicity remains unclear. The aim of our study was to explore the link among Pb exposure, behavior changes, and gut microbiota. Methods: Using Drosophila melanogaster as model, climbing assay, social avoidance, social space, and short-term memory analysis were preformed to study the behavioral changes in flies exposed to Pb and their offspring. 16S rRNA sequencing was used to explore the changes in the gut microbiota of the flies with/without Pb-exposure. Results: The crawling ability, memory, and social interactions of Pb-exposed parent flies decreased significantly. For the offspring, behaviors were more significantly affected in male offspring whose male parent was exposed to Pb. The alpha diversity and the beta diversity of gut microbiota were significantly different between the Pbexposed flies and the controls, as well as between the male offspring and the controls. Two genera, Lactobacillus and Bifidobacterium were found significantly decreased in the Pb-exposed flies when compared to the controls and significantly correlated with the learning and memory. Four genera, Bilophila, Coprococcus, Desulfovibrio, and Ruminococcus were found depleted in the female offspring of the Pb-exposed flies. Conclusions: Lead exposure resulted in defective behavior and alteration of gut microbiota composition in flies and their offspring, alteration in gut microbiota might be the link between behavioral changes induced by Pb-exposure. K E Y W O R D S behavior analysis, Drosophila melanogaster, gut microbiota, lead exposure 700 | SUN et al.

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Sea buckthorn (Hippophae rhamnoides L.) fermentation liquid protects against alcoholic liver disease linked to regulation of liver metabolome and the abundance of gut microbiota

Ran

Guo

et al. 2020

J Sci Food Agric

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BACKGROUND Alcoholic liver disease (ALD) refers to liver damage caused by long‐term heavy drinking, which causes oxidative stress and changes in gut microbiota. In this paper, we investigated the hepatoprotective effect of sea buckthorn fermentation liquid on ALD in mice and the interaction between ALD and gut microbiota using animal experiments and gut microbiota measurements. RESULTS We found that the contents of total flavonoids, total triterpenes and related short‐chain fatty acids (SCFAs) in sea buckthorn fermentation liquid (SFL) were significantly greater. Liver index, kidney index, spleen index, serum indexes of liver injury – alanine aminotransferase (ALT) and spartate aminotransferase (AST), inflammatory factors in liver tissues – tumor necrosis factor‐α (TNF‐α) and interleukin‐6 (IL‐6), oxidation indexes – malondialdehyde (MDA) and superoxide dismutase (SOD), and lipid metabolism indexes – high‐density liptein cholesterol (HDL‐C), low density lipoprotein cholesterol (LDL‐C), and triglyceride (TG), suggested that SFL significantly ameliorates liver injury caused by alcohol. By measuring gut microbiota in mice feces samples, we found that the high‐dose group of SFL reversed the declining trend of the gut microbiota Firmicutes/Bacteroidetes (F/B) ratio caused by alcohol, reducing the number of gram‐negative bacteroidetes. Patescibacteria was tightly connected with the indicators of ALD. At the genus level, high‐dose SFL significantly downregulated Akkermansia, Turicibacter, Alistipes and Ruminiclostridium, and improved the abundance of beneficial bacteria in Lactobacillus. In addition, Alistipes and Ruminiclostridium was closely connected with the indicators of ALD. CONCLUSION Sea buckthorn fermentation liquid protected against alcoholic liver disease and modulated the composition of gut microbiota. © 2020 Society of Chemical Industry

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Bacillus coagulans R11 maintained intestinal villus health and decreased intestinal injury in lead-exposed mice by regulating the intestinal microbiota and influenced the function of faecal microRNAs

Cited by 35 publications

References 40 publications

The Combination of Lead and Bacillus coagulans R11 Increased the Concentration of Alpha-Solanine in the Cecum of Laying Hens and the Pathogens Abundance Decreased

The Combination of Lead and Bacillus coagulans R11 Increased the Concentration of Alpha-Solanine in the Cecum of Laying Hens and the Pathogens Abundance Decreased

Lead exposure results in defective behavior as well as alteration of gut microbiota composition in flies and their offsprings

Sea buckthorn (Hippophae rhamnoides L.) fermentation liquid protects against alcoholic liver disease linked to regulation of liver metabolome and the abundance of gut microbiota

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