Bacillary dysentery from World War 1 and NCTC1, the first bacterial isolate in the National Collection

Mather, Alison E.; Baker, Kate S.; McGregor, Hannah; Coupland, Paul; Mather, Pamela L; Deheer-Graham, Ana; Parkhill, Julian; Bracegirdle, P.; Russell, Julie E.; Thomson, Nicholas R.

doi:10.1016/s0140-6736(14)61790-6

Cited by 15 publications

(10 citation statements)

References 2 publications

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“…The first bacterial isolate deposited in the United Kingdom's National Collection of Type Cultures (NCTC) was a strain of the dysentery-causing bacterium Shigella flexneri that killed a young soldier in World War I. Despite being isolated in 1915 (long before the discovery and use of antibiotics), this human pathogen was found to carry resistance genes for penicillin and erythromycin when it was revived and sequenced in 2014 (Mather et al 2014).…”

Section: Links To the Environment And Gene Mobilizationmentioning

confidence: 99%

“…Besides the studies mentioned in the introduction to this review (Mather et al 2014;Warinner et al 2014), few instances of resistance have been found in the limited number of sequenced of ancient microorganisms from human samples. The genome sequence of Vibrio cholera from 19th century Philadelphia did not reveal any candidate resistance genes apart from efflux , nor were any specific resistance genes found in several strains of the plague-causing bacillus Yersinia pestis isolated from the plague of Justinian (541-543 AD) (Wagner et al 2014).…”

Section: Ancient Antibiotic Resistancementioning

confidence: 99%

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The Prehistory of Antibiotic Resistance

Perry

Waglechner

Wright

2016

Cold Spring Harb Perspect Med

149

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Section: Links To the Environment And Gene Mobilizationmentioning

confidence: 99%

Section: Ancient Antibiotic Resistancementioning

confidence: 99%

The Prehistory of Antibiotic Resistance

Perry

Waglechner

Wright

2016

Cold Spring Harb Perspect Med

149

View full text Add to dashboard Cite

“…Shigella is one of the major pathogens that causes diarrheal diseases in humans and animals [1,2]. Since the rst highly toxigenic Shigella dysenteriae was isolated in 1898 [3], four species has been described in Shigella genus.…”

Section: Introductionmentioning

confidence: 99%

Epidemic and molecular characterization of fluoroquinolone-resistant Shigella dysenteriae 1 isolates from calves with diarrhea

Cao

Wang

Zhang

et al. 2020

Preprint

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Background: The widespread distribution of antimicrobial-resistant Shigella has become a recurrent challenge in many parts of the developing world. Previous studies indicate that the host of Shigella has expanded from humans to animals. This study aimed to investigate the prevalence of fluoroquinolone resistance and associated molecular characterization of S. dysenteriae 1 isolated from calves. Results: All 38 unduplicated S. dysenteriae 1 isolates were collected from calves in Gansu Province from October 2014 to December 2016. According to MLST and PFGE analysis, these isolates were separated into 4 and 28 genotypes, respectively. The most common STs identified were ST228 (34.21%, 13/38) and ST229 (39.47%, 15/38), which were first found in the present study. All isolates harbored virulence genes, and the incidence of the seven virulence genes were ipaH (100%), ipaBCD (92.11%), stx (73.68%), ial (57.89%), sen (28.95%), set1A and set1B. According to the results of antimicrobial susceptibilities, 76.32% (29/38) were resistant to fluoroquinolone and showed multidrug resistance. In a study on the polymorphism of quinolone resistance–determining region (QRDR) of gyrA/B and parC/E genes, we identified two mutations in gyrA (Ser83→Leu and Asp87→Asn) and parC (Ser80→Ile and Ser83→Leu), respectively. Among them, 55.17% (16/29) of resistant strains had the gyrA point mutations (Ser83→Leu) and parC point mutation (Ser83→Leu). Moreover, 41.38% (12/29) of isolates had all five point mutations of gyrA and parC. In addition, the prevalence of the plasmid-mediated quinolone resistance (PMQR) determinant genes was also investigated. All 29 fluoroquinolone-resistant isolates were positive for the aac(6’)-Ib-cr gene but negative for qepA, except SD001. In addition, only 6 (20.69%, 6/29) isolates harbored the qnr gene, including two with qnrB (6.90%, 2/29) and four with qnrS (13.79%, 4/29). Conclusion: Given the increased common emergence of multidrug resistant isolates, uninterrupted surveillance will be necessary to understand the actual epidemic burden and control this infection.

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Section: Introductionmentioning

confidence: 99%

Epidemic and molecular characterization of fluoroquinolone-resistant Shigella dysenteriae 1 isolates from calves with diarrhea

Cao

Wang

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: The widespread distribution of antimicrobial-resistant Shigella has become a recurrent challenge in many parts of the developing world. Previous studies indicate that the host of Shigella has expanded from humans to animals. This study aimed to investigate the prevalence of fluoroquinolone resistance and associated molecular characterization of S. dysenteriae 1 isolated from calves. Results: All 38 unduplicated S. dysenteriae 1 isolates were collected from calves in Gansu Province from October 2014 to December 2016. According to MLST and PFGE analysis, these isolates were separated into 4 and 28 genotypes, respectively. The most common STs identified were ST228 (34.21%, 13/38) and ST229 (39.47%, 15/38), which were first found in the present study. All isolates harbored virulence genes, and the incidence of the seven virulence genes were ipaH (100%), ipaBCD (92.11%), stx (73.68%), ial (57.89%), sen (28.95%), set1A and set1B (0%). According to the results of antimicrobial susceptibilities, 76.32% (29/38) were resistant to fluoroquinolone and showed multidrug resistance. In a study on the polymorphism of quinolone resistance–determining region (QRDR) of gyrA/B and parC/E genes, we identified two mutations in gyrA (Ser83→Leu and Asp87→Asn) and parC (Ser80→Ile and Ser83→Leu), respectively. Among them, 55.17% (16/29) of resistant strains had the gyrA point mutations (Ser83→Leu) and parC point mutation (Ser83→Leu). Moreover, 41.38% (12/29) of isolates had all five point mutations of gyrA and parC. In addition, the prevalence of the plasmid-mediated quinolone resistance (PMQR) determinant genes was also investigated. All 29 fluoroquinolone-resistant isolates were positive for the aac(6’)-Ib-cr gene but negative for qepA, except for SD001. In addition, only 6 (20.69%, 6/29) isolates harbored the qnr gene, including two with qnrB (6.90%, 2/29) and four with qnrS (13.79%, 4/29). Conclusion: Given the increased common emergence of multidrug resistant isolates, uninterrupted surveillance will be necessary to understand the actual epidemic burden and control this infection.

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Bacillary dysentery from World War 1 and NCTC1, the first bacterial isolate in the National Collection

Cited by 15 publications

References 2 publications

The Prehistory of Antibiotic Resistance

The Prehistory of Antibiotic Resistance

Epidemic and molecular characterization of fluoroquinolone-resistant Shigella dysenteriae 1 isolates from calves with diarrhea

Epidemic and molecular characterization of fluoroquinolone-resistant Shigella dysenteriae 1 isolates from calves with diarrhea

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