Babesia spp. infection in Boophilus microplus engorged females and eggs in São Paulo State, Brazil

Oliveira, M. C. de S.; Oliveira-Sequeira, T.C.G.; Araújo, João Pessoa; Amarante, Alessandro Francisco Talamini do; Oliveira, Henrique Nunes de

doi:10.1016/j.vetpar.2005.03.007

Cited by 44 publications

(27 citation statements)

References 20 publications

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“…However, a comparatively higher prevalence of B. babesiosis has been reported from various regions viz: Pakistan (29 %) by Chaudhry et al (2010), Zimbabwe (35 %) by Smeenk et al (2000) using PCR assays. A very high sensitivity of PCR/ nPCR for the detection of B. bigemina was reported by Oliveira et al (2005) in both cattle and R. microplus and Silva et al (2009) in cattle. In India, only few reports employing PCR based assay to know the status of the disease are available from different areas of the country (Ravindran et al 2002;Singh et al 2007Nair et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Also, a high degree of sero-prevalence (59.47 %) has been reported using IFAT in female cattle of Ludhiana district of Punjab (Singh et al 2007). Therefore, owing to the higher sensitivity of nested PCR (Oliveira et al 2005) and abundance of specific vector, higher prevalence value of B. bigemina infection in the cattle of Punjab is not surprising. B. bigemina is protozoa of major concern in Indian cattle herds, parasitizing both cattle and buffaloes (Muraleedharan et al 1984).…”

Section: Discussionmentioning

confidence: 99%

“…Alternative diagnostic approaches like indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) are capable of detecting the antibody in carrier animals but suffer serious drawbacks of cross-reaction between B. bigemina and B. bovis and the lack of discrimination between previous exposure and current infections (Wagner et al 1992). However, recently a number of molecular techniques have been described that can detect and differentiate various species of Babesia in carrier animals (Oliveira et al 2005;Buling et al 2007;Ravindran et al 2008;Singh et al 2010). The application of PCR-based assays for epidemiological studies of babesiosis is still incipient, but its characteristics of high sensitivity and specificity have been verified by several authors for the detection of infection (Almeria et al 2001;Oliveira et al 2005;Ravindran et al 2008;Singh et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…However, recently a number of molecular techniques have been described that can detect and differentiate various species of Babesia in carrier animals (Oliveira et al 2005;Buling et al 2007;Ravindran et al 2008;Singh et al 2010). The application of PCR-based assays for epidemiological studies of babesiosis is still incipient, but its characteristics of high sensitivity and specificity have been verified by several authors for the detection of infection (Almeria et al 2001;Oliveira et al 2005;Ravindran et al 2008;Singh et al 2010). Nested PCR based studies to ascertain the status of babesiosis in healthy carrier animals exhibiting low parasitaemia has not been carried yet, especially from this part of the country.…”

Section: Introductionmentioning

confidence: 99%

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Molecular detection of Babesia bigemina infection in apparently healthy cattle of central plain zone of Punjab

Bhat

Singh

et al. 2014

J Parasit Dis

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Bovine babesiosis is an important tick-borne disease caused by the parasites belonging to the genus Babesia, distributed worldwide and infecting a wide range of domesticated and wild cattle, occasionally man. The present study was conducted to determine the prevalence of B. bigemina infection in apparently healthy cattle from central plain zone of Punjab, India. Examination of Giemsa-stained peripheral thin blood smears revealed 2.45 % (5/204) animals to be positive for piroplasms of B. bigemina. However, genomic DNA isolated from these blood samples when subjected to primary PCR revealed a positivity of 7.35 % (15/204) as detected by the amplification of a 278-bp product in the agarose gel. PCR products obtained from the primary PCR of B. bigemina, when employed as template in nested PCR produced the amplicons of desired size (170 bp) was detected in 30.39 % (62/ 204) of the samples. It can thus be concluded that B. bigemina infection is prevalent in apparently healthy cattle population of this region and PCR assays can serve as a valuable tool for epidemiological studies in endemic areas.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular detection of Babesia bigemina infection in apparently healthy cattle of central plain zone of Punjab

Bhat

Singh

et al. 2014

J Parasit Dis

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“…Thus, we are sure that the acaricide has no confounding effect on our findings. (Oliveira et al, 2005) so that the females adult would have little chance to infect itself. Cafrune et al (1993) described the vulnerability of tick severely infected by Babesia bovis so that these ticks die.…”

Section: B: Dorsal View (Male)mentioning

confidence: 99%

Cattle ticks population and prevalence of <i>Babesia spp</i> amongst it vector: <i>Rhipicephalus (Boophilus) microplus</i> in a zone of Ivory Coast

Touré¹,

Komoin-Oka²,

Sylla³

2012

Int. J. Bio. Chem. Sci

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The aim of this survey is to evaluate prevalence of Babesia spp in Rhipicephalus (Boophilus) microplus and the importance of ticks species existing in Bingerville area. In this cross sectional study, thirty six cattle from 6 farms have been sampled in Bingerville area (Ivory coast Southeast). From 511 ticks collected, 102 Ambyomma spp (19.97%) and 409 Rhipicephalus (Boophilus) microplus (80.03%) were identified. Then, the prevalence of Babesia spp parasites amongst Rhipicephalus (Boophilus) microplus was focused. With 120 engorged females of Rhipicephalus (Boophilus) microplus, 24 pools of 5 ticks each were done. The Giemsa stain test had shown prevalences of 8.33% and 1.73% of Babesia spp respectively in pool and individual levels.

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RNA interference of cytosolic leucine aminopeptidase reduces fecundity in the hard tick, Haemaphysalis longicornis

et al. 2006

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Ticks are effective vectors of pathogens because of their blood feeding and high fecundity. This high fecundity is related to the size of the blood meal. Therefore, knowledge of how blood proteins are degraded and converted to proteins, including yolk protein, is important for the development of ways to inhibit the utilization of blood proteins by ticks. RNA interference (RNAi) is becoming a powerful post-transcriptional gene silencing technique that provides insight into gene function. We constructed a double-stranded RNA (dsRNA) based on a previously cloned Haemaphysalis longicornis leucine aminopeptidase (HlLAP) gene to reevaluate the biological role in tick blood digestion. Gene specific transcriptional, translational, and functional disruptions were achieved by the introduction of dsRNA into the ticks. Significantly delayed onset of egg-laying and reduced egg oviposition resulted from the RNAi for the HlLAP gene. These results suggest that HlLAP actually works as a blood digestive enzyme and affects tick fecundity via unknown mechanisms. The reduction of egg oviposition may be caused by a decrease in nutrients, especially free amino acids generated by HlLAP, from the blood meal. This is the first report of an impact on tick reproduction caused by gene silencing of a blood digestion-related molecule.

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Babesia spp. infection in Boophilus microplus engorged females and eggs in São Paulo State, Brazil

Cited by 44 publications

References 20 publications

Molecular detection of Babesia bigemina infection in apparently healthy cattle of central plain zone of Punjab

Molecular detection of Babesia bigemina infection in apparently healthy cattle of central plain zone of Punjab

Cattle ticks population and prevalence of <i>Babesia spp</i> amongst it vector: <i>Rhipicephalus (Boophilus) microplus</i> in a zone of Ivory Coast

RNA interference of cytosolic leucine aminopeptidase reduces fecundity in the hard tick, Haemaphysalis longicornis

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