In this study, the silk floss sheet (SFS) modified with β‐cyclodextrin (β‐CD) is used to adsorb various dyes (anionic: Acid Red 33/ AR33, cationic: Basic Blue 9/ BB9, and non‐ionic: Disperse Yellow 26/ DY26) from colored solutions. The various analyses (fourier transform infrared spectroscopy (FTIR), X‐ray diffraction (XRD), ultraviolet‐visible spectroscopy (UV‐Vis), and elemental analysis) are used to study the inclusion complex between β‐CD and the mentioned dyes. In addition, the influence of experimental parameters on the adsorption process (such as dye molecular size, adsorbent dosage, initial dye concentration, contact time, and pH) is assessed. The results show that the molecular size and pH have more effect on the adsorption capacity of SFS. It is found that optimum adsorption of acid and disperse dyes occurrs at pH 4, while basic dye can adsorb at around pH 10. Also, the experimental data have a good fit with the Langmuir equation and the kinetic data can correlate with the second‐order equations. Finally, the desorption studies show that this natural adsorbent can be reused many times without any change in its adsorption capacity.