Apyap1affects aflatoxin biosynthesis duringAspergillus parasiticusgrowth in maize seeds

Reverberi, Massimo; Zjalić, Slaven; Punelli, Federico; Ricelli, Alessandra; Fabbri, Anna Adele; Fanelli, C.

doi:10.1080/02652030701553244

Cited by 48 publications

(38 citation statements)

References 19 publications

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“…S4) than the wild-type. NapA orthologues in A. ochraceus and A. parasiticus have been reported to be negative regulators of ochratoxin and aflatoxin in these two species, thus fitting with our observations (Reverberi et al, 2007(Reverberi et al, , 2012. In solid-state cultivation, OE : : zipA also showed some increase in sterigmatocystin (Fig.…”

Section: Discussionsupporting

confidence: 80%

“…Yap-like bZIPs have also been associated with negative regulation of secondary metabolites. For example, deletion of Aoyap1 and Apyap1 in A. ochraceus and A. parasiticus resulted in increases of ochratoxin and aflatoxin, respectively (Reverberi et al, 2007(Reverberi et al, , 2012. These latter two studies led to the hypothesis that fungal SM can be triggered by stress-response pathways.…”

Section: Introductionmentioning

confidence: 96%

“…Whereas RsmA regulation of sterigmatocystin is positive via DNA binding of aflR (Yin et al, 2012), studies of NapA deletants in A. ochraceus and A. parasiticus (Aoyap1 and Apyap1, respectively) suggested that the effect of this bZIP factor on SM was indirect. Specifically, a hypothesis was proposed that the loss of Aoyap1/Apyap1 disabled the redox balance in fungi, leading to activation of secondary metabolite synthesis (Reverberi et al, 2007(Reverberi et al, , 2008(Reverberi et al, , 2012. We therefore set out to examine the relationship of oxidative stress regulation and sterigmatocystin production more exhaustively in the napA mutants.…”

Section: Napa Involvement In Sm Is Stress-relatedmentioning

confidence: 99%

“…Similar to the reported roles in yeast, these proteins typically are associated with resistance to ROS or antifungals. The Yap1 orthologues Afyap1, Aoyap1, Apyap1 and MoAP1 in the human pathogen Aspergillus fumigatus, the mycotoxic pathogens Aspergillus ochraceus and Aspergillus parasiticus, and the rice pathogen Magnaporthe oryzae, respectively, all mediate the oxidative stress response, and in the latter pathogen, MoAP1 is critical for pathogenicity (Guo et al, 2011;Lessing et al, 2007;Qiao et al, 2008;Reverberi et al, 2007Reverberi et al, , 2012. Yap-like proteins in Aspergillus nidulans and Neurospora crassa have also been characterized in ROS and heavy metal resistance Tian et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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bZIP transcription factors affecting secondary metabolism, sexual development and stress responses in Aspergillus nidulans

et al. 2013

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The eukaryotic basic leucine zipper (bZIP) transcription factors play critical roles in the organismal response to the environment. Recently, a novel YAP-like bZIP, restorer of secondary metabolism A (RsmA), was found in a suppressor screen of an Aspergillus nidulans secondary metabolism (SM) mutant in which overexpression of rsmA was found to partially remediate loss of SM in Velvet Complex mutants. The Velvet Complex is a conserved fungal transcriptional heteromer that couples SM with sexual development in fungi. Here we characterized and contrasted SM in mutants of RsmA and four other A. nidulans bZIP proteins (NapA, ZipA, ZipB and ZipC) with predicted DNA binding motifs similar to RsmA. Only two overexpression mutants exhibited both SM and sexual abnormalities that were noteworthy: OE : : rsmA resulted in a 100-fold increase in sterigmatocystin and a near loss of meiotic spore production. OE : : napA displayed decreased production of sterigmatocystin, emericellin, asperthecin, shamixanthone and epishamixanthone, coupled with a shift from sexual to asexual development. Quantification of bZIP homodimer and heterodimer formation using fluorescence resonance energy transfer (FRET) suggested that these proteins preferentially self-associate.

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 96%

Section: Napa Involvement In Sm Is Stress-relatedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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bZIP transcription factors affecting secondary metabolism, sexual development and stress responses in Aspergillus nidulans

et al. 2013

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“…An intriguing relation between oxidative stress, secondary metabolite production and sexual development has been unfolding during the last few years in Aspergillus, which was initiated by the work of Reverberi et al (2007) on Aspergillus parasiticus and Aspergillus ochraceus, and further studied by many others (Baidya et al, 2014;Grintzalis et al, 2014;Reverberi et al, 2012;Wartenberg et al, 2012;Yin et al, 2013). In A. nidulans, the stress response proteins NapA and DlpA have been shown to play a role in developmental processes and sterigmatocystin production (Wartenberg et al, 2012;Yin et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Further characterization of the role of the mitochondrial high-mobility group box protein in the intracellular redox environment of Aspergillus nidulans

et al. 2015

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zé p fasor 52, Hungary HmbB, a predominantly mitochondrial high-mobility group box (HMGB) protein, of Aspergillus nidulans affects diverse biological activities, such as sterigmatocystin production, the maintenance of mitochondrial DNA copy number, germination of asexual and sexual spores, and protection against oxidative stress agents. We hypothesized that the latter correlates with an unbalanced intracellular redox state, in which case, a not yet fully characterized physiological function could be attributed to this mitochondrial HMGB protein. Here, we studied the intracellular redox environment and oxidative stress tolerance in hmbB + and hmbBD strains under normal and oxidative stress conditions by measuring glutathione redox couple, intracellular reactive oxygen species (ROS) content and ROS-protecting enzyme activities. Our results revealed that the intracellular redox environment is different in hmbBD conidia and mycelia from that of hmbB + , and shed light on the seemingly contradictory difference in the tolerance of hmbBD mycelia to diamide and menadione oxidative stressors.

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Waking Sleeping Pathways in Filamentous Fungi

Spraker

Keller

2014

Natural Products

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Apyap1affects aflatoxin biosynthesis duringAspergillus parasiticusgrowth in maize seeds

Cited by 48 publications

References 19 publications

bZIP transcription factors affecting secondary metabolism, sexual development and stress responses in Aspergillus nidulans

bZIP transcription factors affecting secondary metabolism, sexual development and stress responses in Aspergillus nidulans

Further characterization of the role of the mitochondrial high-mobility group box protein in the intracellular redox environment of Aspergillus nidulans

Waking Sleeping Pathways in Filamentous Fungi

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