B Cells in Leprosy

Kreisler, M.; A, Arnaiz; Cruz, Elena de la; Bootello, A; Pérez, Berta Fernández

doi:10.1016/s0140-6736(73)91939-9

Cited by 1 publication

(1 citation statement)

References 5 publications

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“…These outcomes seem to be dependent both upon cellular conditions (e.g., stress due to nutritional deficiency) [15,16] and on irradiation parameters (wavelength, fluence and rate fluence, for instance) [17]. With such well-documented effects on cell proliferation, LLLT faces opposition when light is clinically applied to body sites that present tumor cells or lesions with metastatic potential, mainly because literature is still contradictory about this issue [19][20][21][22]. For example, Frigo et al observed that fluence of 150 J cm -2 did not increase melanoma cell viability, but 1050 J cm -2 showed a significant increase in tumor mass volume in a murine model [23].…”

Section: Discussionmentioning

confidence: 99%

Exploring the effects of low‐level laser therapy on fibroblasts and tumor cells following gamma radiation exposure

Silva¹,

Cabral²,

Camargo³

et al. 2016

Journal of Biophotonics

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Ionizing radiation (IR) induces DNA damage and low-level laser therapy (LLLT) has been investigated to prevent or repair detrimental outcomes resulting from IR exposure. Few in vitro studies, however, explore the biological mechanisms underlying those LLLT benefits. Thus, in this work, fibroblasts and tumor cells are submitted to IR with doses of 2.5 Gy and 10 Gy. After twenty-four-h, the cells are exposed to LLLT with fluences of 30 J cm , 90 J cm , and 150 J cm . Cellular viability, cell cycle phases, cell proliferation index and senescence are evaluated on days 1 and 4 after LLLT irradiation. For fibroblasts, LLLT promotes - in a fluence-dependent manner - increments in cell viability and proliferation, while a reduction in the senescence was observed. Regarding tumor cells, no influences of LLLT on cell viability are noticed. Whereas LLLT enhances cell populations in S and G /M cell cycle phases for both cellular lines, a decrease in proliferation and increase in senescence was verified only for tumor cells. Putting together, the results suggest that fibroblasts and tumor cells present different responses to LLLT following exposure to gamma-radiation, and these promising results should stimulate further investigations. Senescence of tumor cells and fibroblasts on the 4 day after ionizing radiation (IR) and low-level laser therapy (LLLT) exposures. The number of senescent cells increased significantly for tumor cells (a) while for fibroblasts no increment was observed (b). The blue collor indicates senescence activity.

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