Avaliações clínica, ecográfica e anatomofisiológica do alotransplante parcial de vesícula urinária com células-tronco mesenquimais alogênicas derivadas do tecido adiposo em coelhos

Filho, Saulo Tadeu Lemos Pinto; Oliveira, Marília Teresa de; Aramburú, Jaime Sardá; Glanzner, Werner Giehl; Silva, T. O.; Feranti, João P.S.; Gonçalves, P. B. D.; Cruz, I. B. M.; Brun, Maurício Veloso; Pippi, Ney Luís

doi:10.1590/1678-4162-8148

Cited by 3 publications

(6 citation statements)

References 14 publications

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“…Haematuria happened 24 hours after surgery, but no on the subsequent days. As a verified by previous studies (Pinto Filho et al 2015Filho et al , 2016, it is a common finding during early postoperative of urinary bladder surgery. WBC showed no significant differences between pre-and postoperative period and between groups; during this time, leukocyte counting was in the normal range for the specie.…”

Section: Discussionsupporting

confidence: 67%

“…Cyclosporine, used in the CGcy, is preferred by some researchers as an immunosuppressive agent in transplanted animals (Teixeira et al 2007, Chaves et al 2008). Pinto Filho et al (2015) used cyclosporine to prevent rejection for 30 days after partial cystectomy surgery in rabbits and found no signs of implant rejection in all treated animals. Chaves et al (2008) verified that cyclosporine prolonged survival of skin grafts in rabbits.…”

Section: Discussionmentioning

confidence: 99%

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Expression patterns of mesenchymal stem cell-specific proteins in adipose tissue-derived cells: possible immunosuppressing agent in partial allograft for restoring the urinary bladder in rabbits

et al. 2018

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Adipose tissue-derived stem cells (ADSCs) are an attractive source of mesenchymal stem cells (MSCs) for use in tissue engineering and clinical applications. This paper focuses on the characterization of ADSCs used as immunosuppressive agent in rabbits undergoing partial allograft for urine bladder restorage. For this study highlighted the characterization of the ADSCs used as immunosuppressive agents in rabbits submitted to partial allograft for restoration of the urinary vesicle, using 25 animals, six months old, New Zealand. ADSCs at the third peal were characterized by the MSC-specific CD105, CD73 and CD90 expression and by the absence of the hematopoietic marker CD45, as revealed by flow cytometry analysis. Moreover, ADSCs were efficient in preventing allograft rejection from the urinary bladder, as judged by biochemical, clinical and ultrasonography analysis. Together, these results compose characterization of protein expression profiles and immunosuppressive functionality of ADSCs in rabbits, which had undergone partial allografts of the urinary bladder, foreseeing future applications in clinical practice.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Expression patterns of mesenchymal stem cell-specific proteins in adipose tissue-derived cells: possible immunosuppressing agent in partial allograft for restoring the urinary bladder in rabbits

et al. 2018

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“…Several authors have reported a simple continuous pattern, the same as we used to suture the graft to the bladder wall and had no urine extravasation (ALBRECHT et al, 2006;OLIVEIRA et al, 2008;PINTO FILHO et al, 2015). However, IIJIMA et al ( 2007) observed uroperitoneum followed by death with the same suture pattern.…”

Section: Rodrigues Et Almentioning

confidence: 80%

“…No animal had surgical wound complications, that is no local sensitivity, hyperthermia, suture dehiscence, behavior changes or any signs of infection or graft rejection as also seen by PINTO FILHO et al (2015) in rabbits underwent bladder allograft. This fact can be related to an adequate surgical technique and to the tunica albuginea graft satisfactory consolidation with the receptor organ.…”

Section: Rodrigues Et Almentioning

confidence: 96%

Bovine tunica albuginea conserved in honey as xenograft for cystoplasty in rats

et al. 2021

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Urinary bladder damages leading to few viable bladder tissue available might demand a challenging reconstructive surgery. In this context, biomaterials are valid alternatives for bladder reconstruction. This study evaluated the bovine tunica albuginea fragment as graft material for cystoplasty in rats and honey-preserved implant viability.Thirty Wistar rats were assigned to two groups: (1) a test group (T) with a circular 1.0-cm-diameterbovine tunica albuginea graft application in the apex region by a continuous absorbable (Polyglactin 910 5-0) suture with stitching of all bladder layers and (2) a simulation group (S) in which animals underwent only partial cystectomy. In addition, each of these groups was further divided into three subgroups according to euthanasia period on post-surgery day 7, 15 and 30.Two animals had self-limiting hematuria at postsurgical period. At necropsy, frequent crystals and adhesion to the peritoneum were observed. At the histopathological evaluation, animals from the T group euthanized by 15th postoperative day had layers disorganization and initial muscle development, while T group rats euthanized by 30th postoperative day showed complete urothelization. Urothelization pattern was similar in both groups. Moreover, the muscular layer formation was present in both groups, but more evident in S group animals. Nevertheless, inflammatory infiltrate and neovascularization were remarkably more intense in T group rats.It might be concluded that bovine tunica albuginea graft was successful in repairing rats’ bladder, being a good biomaterial option in reconstructive urinary vesicle surgery.

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“…Pinto Filho., et al [21] reported good recovery of surgical wound with no local sensitivity, no hyperthermia, or suture dehiscence in animals. During necropsy, no signs of infection or graft rejection were recorded indicating good application of surgical technique and adequate use of biomaterial as graft.…”

Section: Resultsmentioning

confidence: 97%

Ovine Tunica Albuginea as Xenograft for Cystoplasty in Rats

Ferreira¹,

Castañon²,

Medeiros³

et al. 2020

Act Sci VetSci

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Avaliações clínica, ecográfica e anatomofisiológica do alotransplante parcial de vesícula urinária com células-tronco mesenquimais alogênicas derivadas do tecido adiposo em coelhos

Abstract: Avaliações clínica, ecográfica e anatomofisiológica do alotransplante parcial de vesícula urinária com células-tronco mesenquimais alogênicas derivadas do tecido adiposo em coelhos [Clinical, sonographic, anatomic

Cited by 3 publications

References 14 publications

Expression patterns of mesenchymal stem cell-specific proteins in adipose tissue-derived cells: possible immunosuppressing agent in partial allograft for restoring the urinary bladder in rabbits

Expression patterns of mesenchymal stem cell-specific proteins in adipose tissue-derived cells: possible immunosuppressing agent in partial allograft for restoring the urinary bladder in rabbits

Bovine tunica albuginea conserved in honey as xenograft for cystoplasty in rats

Ovine Tunica Albuginea as Xenograft for Cystoplasty in Rats

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