2013
DOI: 10.1590/s0100-736x2013000300006
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Abstract: Objetivou-se com esse trabalho realizar um inquérito epidemiológico da infecção por Mycobacterium avium subsp. paratuberculosis (MAP) em bovinos leiteiros da microrregião de Garanhuns, Pernambuco, Brasil. Para este estudo foram coletadas amostras sanguíneas de 408 animais, provenientes de 19 rebanhos localizados em 15 municípios. O exame sorológico foi realizado por Ensaio Imunoenzimático (ELISA) indireto para detecção de anticorpos frente ao MAP. Em todas as propriedades, foi aplicado um questionário investig… Show more

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Cited by 10 publications
(7 citation statements)
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References 18 publications
(20 reference statements)
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“…However, MAP has been detected in raw and pasteurized milk (Grant et al, 2002;O'Reilly et al, 2004;Ayele et al, 2005;Slana et al, 2009;Okura et al, 2012), and the habit of consuming raw cow milk is common in the State of Paraíba, predominantly in family farms. Therefore, the confirmation of a zoonotic link between MAP and CD could have serious consequences for the dairy industry of the State of Paraíba and Brazil, the latter of which assuming a prominent position in world milk production, with an estimated milk production of around 37 billion liters.…”
Section: Discussionmentioning
confidence: 99%
“…However, crude numbers suggest that 82 % is the value obtained c Random sampling of herds cannot be confirmed from the information provided in the publication d Proportional selection of herds according to the cattle population of each region cannot be confirmed from the information provided in the publication (Medeiros et al 2012;Sá et al 2013). The lowest prevalence was obtained in Costa Rica with 18.7 % (Dolz et al 1999).…”
Section: Herd-level Prevalence In Cattlementioning
confidence: 99%
“…Previous studies conducted PTB serodiagnoses in other Brazilian states, and found that the occurrence of seropositive animals aged 24 months or older was 34.5% (864/2,504) in Paraíba (VILAR et al, 2015) and to identify risk factors associated with herd-level prevalence for bovine paratuberculosis in the State of Para\u00edba, Northeastern Brazil. The state was divided into three sampling groups: sampling stratum 1 (mesoregion of Sert\u00e3o, 2.7% (11/408) in Pernambuco (SÁ et al, 2013), 14.7% (5/34) in in Rio Grande do Sul (FISS et al, 2015), and 13.2% (165/1,450) in Espírito Santo (COSTA et al, 2010). Compared to the abovementioned results, those of the present study showed a relatively lower prevalence of seropositive animals, although these are results for populations that differed in terms of both their gender and age.…”
Section: Introductionmentioning
confidence: 99%
“…In our study, a higher occurrence of MAP DNA was observed in coalho type cheese samples, perhaps due to the influence of the area where these samples were collected. In the state of Pernambuco, paratuberculosis has been reported with a certain frequency in cattle (Mota et al, 2007;Sá et al, 2013), and its DNA was detected in milk samples in the Garanhuns microregion (Albuquerque et al, 2017). It is also important to highlight that the qPCR technique used in our study has a greater sensitivity and specificity in the detection of this microorganism when compared to conventional PCR, and also has the capacity to quantify the bacteria (Mackay, 2004).…”
Section: Discussionmentioning
confidence: 66%
“…Coalho cheese can be produced in considerable quantity from raw milk, which, if it is not properly hygienic, may not present microbiological safety or standardization (Almeida et al, 2013;Feitosa et al, 2003). In the State of Pernambuco, paratuberculosis has been reported in some studies involving cattle (Mota et al, 2007;Sá et al, 2013) and buffaloes (Mota et al, 2010) in dairy farms. The presence of DNA has also been detected in milk samples of cows from several properties in the same State (Albuquerque et al, 2017).…”
Section: Introduction mentioning
confidence: 99%