Avaliação dos efeitos das raízes de Pfaffia glomerata (Spreng.) Pedersen sobre o tempo de sono e crescimento bacteriano

Vigo, C.L.S.; Narita, E.; Nakamura, Celso V.; Marques, Lays Costa

doi:10.1590/s0102-695x2003000400006

Cited by 6 publications

(2 citation statements)

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“…However, there are few pharmacological studies that prove the efficacy reported in popular medicine. According to Vigo et al (2003) and collaborators, the freeze-dried hydroalcoholic extract of the roots presented stimulant effects in mice, in addiction shows positive effects on learning and memory in aged rats.…”

Section: Introductionmentioning

confidence: 99%

Pfaffia glomerata: Gallic acid and phenolic compounds determination

Silva¹,

Neves²,

Kummer³

et al. 2022

RSD

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Pfaffia glomerata, known as ginseng-brasileiro, is a root widely used in folk medicine, being effective against inflammation, rheumatism, and fatigue, in addition to present aphrodisiacs and antidiabetics properties. Among the classes of substances reported for the specie, the phenolic acids, in special gallic acid, has shown positive activity to learning and memory, having a considerable effect against neurotoxic and pro-oxidant substances. Therefore, the article’s objective was to quantify gallic acid in the chloroform and ethyl acetate fractions from P. glomerata. Thus, the aerial parts of ginseng were collected, dried and subjected to extraction with ethanol followed by fractionation with organic solvents in different polarities, obtaining the hexanic, dichloromethane (CHCl3) and ethyl acetate (AcOEt) fractions. Then, gallic acid was identified by TLC and quantified by HPLC in the ethyl acetate fraction. The total phenolic content was determined in the chloroform and ethyl acetate fractions, presenting values of 70,3 mg/g and 118,6 mg/g, respectively. The gallic acid content determined by HPLC in the AcOEt fraction was 904,15 µg/g, while in the CHCl3 fraction it was not possible to quantify. So, the species can be a source of phenolic compounds for possible applications in the pharmaceutical area.

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Section: Introductionmentioning

confidence: 99%

Pfaffia glomerata: Gallic acid and phenolic compounds determination

Silva¹,

Neves²,

Kummer³

et al. 2022

RSD

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“…Regarding to the central nervous system action, the administration of a crude ethanol extract of P. glomerata by intraperitoneal route produces a depressor effect in the barbiturate sleeping time test and an amnesic effect in adult rodents (De-Paris et al, 2000;Vigo et al, 2003). On the other hand, Marques et al (2004) reported a barbiturate sleeping time decrease and an improvement in learning and memory in old mice chronically treated.…”

Section: Introductionmentioning

confidence: 99%

Hypnotic effect of ecdysterone isolated from Pfaffia glomerata (Spreng.) Pedersen

Fenner¹,

Zimmer²,

Neves³

et al. 2008

Rev. bras. farmacogn.

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RESUMO: "Efeito hipinótico de ecdisterona isolada de Pfaffi a glomerata (Spreng.) Pedersen". Neste trabalho foi avaliado, em roedores, o efeito depressor das frações clorofórmio (CHCl 3 ), acetato de etila (EtOAc) e n-butanol, obtidas das partes subterrâneas de Pfaffi a glomerata, empregando-se o teste de tempo de sono barbitúrico como referência. Somente a fração lipofílica (CHCl 3 :EtOAc, 1:1, m/m) (i.p. 500 mg/kg; v.o. 1000 mg/kg) potenciou o tempo de sono induzido por pentobarbital. A ecdisterona foi isolada e identifi cada como constituinte majoritário (1,4% m/m) desta fração, através de cromatografi a líquida de alta efi ciência e métodos espectroscópicos, respectivamente. Este composto potenciou o tempo de sono barbitúrico (100 mg/kg, i.p.; 400 mg/kg, v.o), sem causar hipotermia. Nestas mesmas doses, a ecdisterona não alterou a performance dos animais no rota-rod, esquiva inibitória e labirinto em cruz-elevado, além de não alterar o padrão de convulsões induzidas por pentilenotetrazol. Este perfi l indica que esta substância, nestas doses, não apresenta perfi l ansiolítico ou neurotóxico. Estes resultados indicam que a ecdisterona é o componente responsável pela ação hipnótica apresentada pela fração lipofílica obtida das partes subterrâneas de P. glomerata. Unitermos:Pfaffi a glomerata, Amaranthaceae, ecdisterona, efeito depressor, sistema nervoso central, tempo de sono induzido por pentobarbital. ABSTRACT:In this study the depressant effect of fractions from P. glomerata was initially evaluated using the mice barbiturate sleeping time test as reference. The fractions tested were the CHCl 3 , the EtOAc, the n-BuOH and the aqueous fraction obtained from P. glomerata subterraneous parts. Only the pretreatment with the lipophilic fraction (CHCl 3 : EtOAc, 1:1, w/w) increased the barbiturate sleeping time (i.p 500 mg/kg; v.o. 1000 mg/kg). Ecdysterone, the main substance isolated from this lipophilic fraction, was identifi ed by spectroscopic methods and its content in the ethanol extract was determined as 1.4% (w/w) by HPLC. In order to investigate the hypothesis of ecdysterone displaying a depressant effect on nervous central system, an evaluation toward the hypnotic-sedative and anxiolytic effects of this drug was carried out. Ecdysterone 100 mg/kg, i.p, increased the barbiturate sleeping time without provoking hypothermia; when administered by oral route its minimal effective dose was 400 mg/kg. On the other hand, ecdysterone (100 mg/kg, i.p; 400 mg/kg, p.o) did not impair motor coordination and was ineffective on pentylenetetrazoleinduced convulsion, elevated plus-maze and step-down inhibitory avoidance tests, indicating that at these doses the drug does not present an anxiolytic profi le and does not cause manifest neurotoxic effects as well. In conclusion, the lipophilic fraction from P. glomerata presents a hypnotic effect being ecdysterone one of the compounds responsible for this CNS activity.

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Phytochemical composition of aerial parts and roots of Pfaffia glomerata (Spreng.) Pedersen and anticholinesterase, antioxidant, and antiglycation activities

Franco,

Justino

et al. 2024

Protoplasma

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Avaliação dos efeitos das raízes de Pfaffia glomerata (Spreng.) Pedersen sobre o tempo de sono e crescimento bacteriano

Cited by 6 publications

References 1 publication

Pfaffia glomerata: Gallic acid and phenolic compounds determination

Pfaffia glomerata: Gallic acid and phenolic compounds determination

Hypnotic effect of ecdysterone isolated from Pfaffia glomerata (Spreng.) Pedersen

Phytochemical composition of aerial parts and roots of Pfaffia glomerata (Spreng.) Pedersen and anticholinesterase, antioxidant, and antiglycation activities

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