"Avaliação de três métodos de extração de DNA de dentes humanos submetidos ao calor"

Remualdo, Vanessa Rosália

doi:10.11606/d.23.2004.tde-21062005-073816

Cited by 4 publications

(4 citation statements)

References 3 publications

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“…Remualdo et al [24] evaluated DNA retrieval from the teeth subjected to heat (200ºC, 400ºC, 500ºC and 600ºC) during 60 minutes, testing 3 different extraction methods (organic; ammonia acetate/isopropanol and silica) . The organic method for genomic DNA extraction useful for 50% of samples, but only at lower temperatures (200ºC and 400ºC).…”

Section: Discussionmentioning

confidence: 99%

Determination of Sex of an Individual by Identifying SRY Gene through PCR Analysis of DNA Extracted From Incinerated Teeth-A Single Blind Pilot Study

Muthusamy¹

2017

IJFSC

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Introduction: Sex determination using skeletal fragments presents a great difficulty to forensic investigators. The teeth are resistant to all conditions due to relatively high degree of physical and chemical resistance of the dental structure. With the development of Polymerised Chain Reaction (PCR), DNA analysis of teeth for sex determination could be performed in bodies that are severely mutilated. Aim and Objective: To determine the reliability of sex determination from incinerated teeth samples using Polymerised Chain Reaction. Materials and Methods: The study was carried on 10 maxillary and mandibular premolars and permanent molars (5male, 5-female). Ten samples were subjected to analysis after the storage period of 6 months. All samples were incinerated at 3000 C for 60 minutes to mimic the forensic scene. DNA extraction was done using Real Genomics YGB 100 DNA extraction kit. DNA quantification was performed using Nano-drop 100 spectrophotometer. SRY gene detection was done by the Real Plex Master Cycler. Results: Gender identification was done by amplification of sex determining region on Y chromosome gene using real time polymerase chain reaction. The prediction rate for males was 100 % and for females was 80% .The difference in the prediction of gender was found to be statistically significant (p value-0.04). Conclusion: DNA extraction was successful in all the incinerated samples which were incinerated till 3000C. Hence, teeth are reliable source for sex determination with 100 % accuracy using Real time PCR.

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Section: Discussionmentioning

confidence: 99%

Determination of Sex of an Individual by Identifying SRY Gene through PCR Analysis of DNA Extracted From Incinerated Teeth-A Single Blind Pilot Study

Muthusamy¹

2017

IJFSC

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“…On the other hand, the material obtained from the hard dental tissues showed satisfactory analysis only by the PCR technique. Remualdo 29 (2004) evaluated the PCR amplification of DNA retrieved from teeth subjected to heat (200°C, 400°C, 500°C and 600°C) during 60 minutes, testing 3 different extraction methods (organic; ammonia acetate/isopropanol and silica). Using the organic method for genomic DNA extraction, 50% of samples subjected to burning were amplified, but only at lower temperatures (200°C and 400°C).…”

Section: Literature Reviewmentioning

confidence: 99%

Use of DNA technology in forensic dentistry

et al. 2007

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The established importance of Forensic Dentistry for human identification, mainly when there is little remaining material to perform such identification (e.g., in fires, explosions, decomposing bodies or skeletonized bodies), has led dentists working with forensic investigation to become more familiar with the new molecular biology techniques. The currently available DNA tests have high reliability and are accepted as legal proofs in courts. This article presents a literature review referring to the main studies on Forensic Dentistry that involve the use of DNA for human identification, and makes an overview of the evolution of this technology in the last years, highlighting the importance of molecular biology in forensic sciences.

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“…It has generally been reported that dental DNA can withstand temperatures up to 400 °C for one hour but that its quantity and quality are superior after exposure to lower temperatures for shorter time periods (1–20 min), facilitating forensic identification 9 . Only a few studies have studied teeth exposed for more than 15 min at temperatures above 90 °C 16 , 17 , 18 , 19 . Most researchers have reported that it is very difficult to extract DNA from teeth after their exposure to temperatures of 200–400 °C 18 , 19 , 20 , and no consensus has been reached on the degree of cremation at which teeth will still yield nuclear DNA signals 7 .…”

Section: Introductionmentioning

confidence: 99%

DNA degradation in human teeth exposed to thermal stress

Lozano-Peral

Rubio

Santos

et al. 2021

Sci Rep

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Human identification from burned remains poses a challenge to forensic laboratories, and DNA profiling is widely used for this purpose. Our aim was to evaluate the effect of temperature on DNA degradation in human teeth. Thirty teeth were exposed to temperatures of 100, 200, or 400 °C for 60 min. DNA was quantified by Real-Time qPCR (Quantifiler Human DNA Quantification Kit) and fluorescence spectroscopy (Qubit 3.0 Fluorometer). DNA degradation was evaluated by using STR markers (AmpFLSTR Identifiler Plus PCR Amplification Kit) to determine the allele and locus dropout, inter-locus balance, and degradation slope (observed (Oa) to expected (Ea) locus peak height ratio against the molecular weight). Most of the genomic DNA was degraded between 100 °C and 200 °C. At 100 °C, locus dropout ratios showed significant differences between the largest loci (FGA, D7S820, D18S51, D16S539, D2S1338 and CSF1PO) and amelogenin. Inter-locus balance values significantly differed between all dye channels except between NED and PET. The dropout ratio between D18S51 (NED) and amelogenin (PET) can be recommended for the evaluation of DNA degradation. The Oa/Ea regression model can predict locus peak heights in DNA degradation (R2 = 0.7881). These findings may be useful to assess the reliability of DNA typing for human identification in teeth subjected to prolonged incineration.

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"Avaliação de três métodos de extração de DNA de dentes humanos submetidos ao calor"

Cited by 4 publications

References 3 publications

Determination of Sex of an Individual by Identifying SRY Gene through PCR Analysis of DNA Extracted From Incinerated Teeth-A Single Blind Pilot Study

Determination of Sex of an Individual by Identifying SRY Gene through PCR Analysis of DNA Extracted From Incinerated Teeth-A Single Blind Pilot Study

Use of DNA technology in forensic dentistry

DNA degradation in human teeth exposed to thermal stress

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