Avaliação da atividade antibacteriana de extratos etanólico e de ciclohexano a partir das flores de camomila (Matricaria chamomilla L.)

Abstract

Recebido para publicação em 23/11/2012 Aceito para publicação em 19/03/2014 RESUMO: Devido à crescente seleção de microrganismos resistentes aos antimicrobianos atuais, temse valorizado a busca por alternativas naturais. O presente estudo teve por objetivo avaliar a atividade antibacteriana de extratos etanólico e de ciclohexano de flores de camomila, espécie vegetal de uso antigo pela medicina tradicional, frente às bactérias ATCC Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli e Salmonella enterica subsp. enterica sorovar Typhimurium pelas técnicas de difusão em ágar e diluição em caldo. Foi observada inibição do crescimento de P. aeruginosa frente ao extrato etanólico bruto (1g/mL) na técnica de diluição em caldo, e confirmada pela técnica de difusão em ágar (halo de inibição de 10 mm de diâmetro). Para as demais bactérias testadas, os extratos e suas diluições não apresentaram efeito bacteriostático em nenhuma das técnicas. Podese concluir que o extrato etanólico bruto da camomila apresentou atividade antibacteriana frente à P. aeruginosa, porém não foi eficaz frente à S. aureus, E. coli e Salmonella enterica subsp. enterica sorovar Typhimurium. Portanto, são necessários novos estudos com diferentes linhagens de microrganismos, com o intuito de corroborar e assegurar os resultados apresentados, para definir o potencial antimicrobiano do extrato da camomila. Palavras-chave: extrato de camomila, atividade biológica, Pseudomonas aeruginosa ABSTRACT: Evaluation of the antibacterial activity of ethanolic and cyclohexane extracts of chamomile flowers (Matricaria chamomilla L.).Due to the growing selection of microorganisms resistant to antimicrobial, the search for natural alternatives has become popular. The objective of the present study was to evaluate the antibacterial activity of ethanolic and cyclohexane extracts of chamomile flowers, a plant species long used by traditional medicine, against ATCC bacteria Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Salmonella enterica subsp. enterica serovar Typhimurium by agar diffusion and broth dilution techniques. Growth of P. aeruginosa was inhibited when crude ethanolic extract (1g/mL) was used broth dilution, and was confirmed by agar diffusion, (10 mm diameter inhibition zone). For the other bacteria tested, the extracts and their dilutions did not show any bacteriostatic effect in any of the techniques. It may be concluded that pure ethanolic extract of chamomile presents antibacterial action against P. aeruginosa, and none against S. aureus, E. coli e Salmonella enterica subsp. enterica sorovar Typhimurium. However, other studies with different strains of microorganisms may be useful in order to corroborate and ensure these results, to define evaluation of the antimicrobial activity of chamomile extract.

Matricaria recutita extract associated with norfloxacin or cephalexin enhances the antimicrobial activity of these drugs against Staphylococcus aureus

“…Some studies considered the kinetics satisfactory when there was a reduction greater than or equal to 2log [10] CFU/ mL, for a period shorter than or equal to 24 h (SHELBURNE et al, 2004). Other authors have demonstrated that an M. recutita extract has excellent in vitro bactericidal activity against Pseudomonas aeruginosa (CARVALHO et al, 2014). OLIVEIRA et al, 2011).…”

“…Samples were obtained from 30 naturally infected lactating bovines. After washing the teat with soap and water, drying it with paper towels and disinfecting the ostium with 70º GL ethyl alcohol, we aseptically collected approximately 5 mL of milk from each mammary quarter reactive to the "California Mastistis Test" (CMT), using a slant tube in horizontal position (CAVALCANTI-DANTAS et al, 2016). Nasal swabs were collected and all samples were stored in sterile threaded tubes, identiied and sent under refrigeration in isothermal boxes, to the Laboratory of Genetics and Microbiology at the Universidade Federal da Paraíba -CCEN, for microbiological examination and identiication of S. aureus.…”

Matricaria Recutita Extract (Chamomile) to reduce Candida Albicans and Entrobacter Cloacae biofilms: in vitro study

“…The goal of this research study was to verify the antibiofilm action of a chamomile formulation, in this case, the hydroalcoholic extract, as it has components with antimicrobial properties, such as the cis-trans-spiroethers, and coumarins, such as the herniarine and umbelliferone [21]. A concern was also to investigate published studies considering microorganisms in the planktonic phase presenting satisfactory results [22,23], low cost and with potential to be used as a mouthwash. In the present study, to determine the minimum concentration of chamomile extract to be used in C. albicans and E. cloacae biofilms, as well as the effectiveness of the chlorhexidine formulation 0.12% (Periogard®), the broth microdilution test for these microorganisms when in the planktonic phase was carried out, which were respectively inhibited at 83 and 100 mg/mL and also by 0.12% chlorhexidine.…”

A ação antimicrobiana dos extratos alcoólicos e aquosos da folha da goiabeira (Psidium guajava L.) no controle de Staphylococcus aureus ATTCC 27922, Escherichia coli ATTCC 25922 e Listeria monocytogenes SCOTT A

“…Programas oficiais de saúde apoiam o uso de plantas medicinais para o tratamento de doenças comuns [3]. De acordo com a Organização Mundial de Saúde (OMS), aproximadamente 80% da população mundial faz uso da medicina tradicional na busca do alívio de sintomas desagradáveis [4] .…”

“…E-mail: alesantana72@yahoo.com.br. ORCID: https://orcid.org/0000-0001-7559-97304 Nutricionista. Departamento de Nutrição, Centro Universitário Estácio do Ceará.…”