Avaliação da ação da Aroeira (Schinus terebinthifolius Raddi) na cicatrização de feridas cirúrgicas em bexiga de ratos

Abstract

RESUMOObjetivo: Avaliar a ação cicatrizante do extrato hidroalcoólico da Aroeira em bexigas de ratos. Métodos: Foram utilizados 40 ratos machos da linhagem Wistar, divididos em dois grupos de 20, denominados grupo Aroeira (GA) e grupo controle (GC). Todos foram submetidos à incisão abdominal mediana com cistotomia de 1 cm, seguida de cistorrafia em plano único com pontos separados de poliglactina 910 5-0 (Vicryl ® ). Após este procedimento, nos animais GA injetou-se 100mg por quilo de peso de extrato hidroalcoólico da Aroeira na cavidade peritoneal e nos GC injetou-se 1 ml por quilo de peso de solução salina a 0,9% . Cada grupo foi dividido em dois sub-grupos de 10 animais de acordo com a data da morte: sub-grupo A3 e C3, sacrificados no 3º dia pós-operatório e sub-grupo A7 e C7, sacrificados no 7º dia. A parede, a cavidade abdominal e a sutura da bexiga foram avaliadas macroscopicamente. Amostras de tecido da bexiga foram retiradas e analisadas histologicamente, utilizando a coloração de Hematoxilina-Eosina (HE) e tricrômio de Masson. Resultados: Na análise macroscópica observou-se infecção na incisão cirúrgica em três ratos do GC e em um do GA, e aderências peritoneais em 29 ratos do GC controle e 17 no GA. A avaliação microscópica mostrou processo inflamatório agudo mais severo no 3° (p=0,045) e no 7° dia (p=0,002). Evidenciou-se ainda diferença estatística nos parâmetros utilizados para a avaliação histológica da cicatrização da bexiga nas variáveis colagenização (p = 0.001), reepitelização (p = 0,046) e neoformação (p = 0) nos subgrupos GC e GA no 3º dia e na variável neoformação vascular (p=0,001) no subgrupo do 7º dia. Conclusão: O uso de extrato hidroalcoólico de Aroeira mostrou efeito cicatrizante favorável nas cistotomias em ratos. Descritores: Schinus terebinthifolius. Cicatrização de Feridas. Bexiga. Ratos ABSTRACT Purpose: To evaluate the hydroalcoholic Aroeira extract in the healing process of surgical incisions in the bladder of rats in an experimental comparative study. Methods: Forty male Wistar rats were used. All of them underwent a median abdominal incision, with an 1cm cistotomy, followed by one plan suture with separated stitches of poliglactine 910 5-0. The animals were then divided into two groups of 20 rats each, and named the Aroeira group (GA) and the control group (GC). In the first one, 80% hydro-alcoholic plant extract was injected in the peritoneal cavity, in a one dose of 100mg/kg. The second group, a 0.9% saline solution was injected in place of Aroeira extract. Each group was divided into two subgroups (SGA and SGC) of 10 animals. According to their scheduled death, they were named subgroups SGA3 and SGC3 when killed in the 3th day after surgery and subgroups SGA7 and SGC7 in the 7th day. The abdominal cavity and the bladder suture were macroscopically evaluated. Results: The microscopic analysis of the surgical incision in the bladder was performed by means of the hematoxilin-eosine stain and the trichrome of Masson. The macroscopic analysis showed an infection in the surgical ...

Schinus terebinthifolius Raddi: chemical composition, biological properties and toxicity

“…Although a large number of studies have evaluated the pharmacological properties of this plant, little attention has been given to the identification (Kassem, et al, 2004) continua... Barbosa, et al, 2007, Bendaoud, et al, 2010, Malik, et al, 1994, Pieribatestti, et al, 1981) (Barbosa, et al, 2007, El-Massry, et al, 2009, Jamal and Augusta, 2001) (Barbosa, et al, 2007, Bauer and Brasil, 1973, Bendaoud, et al, 2010, Jamal and Augusta, 2001, Lloyd, et al, 1977, Malik, et al, 1994, Pieribatestti, et al, 1981) (Barbosa, et al, 2007, Jamal and Augusta, 2001, Lloyd, et al, 1977, Malik, et al, 1994, Pieribatestti, et al, 1981) (Barbosa, et al, 2007, Jamal and Augusta, 2001, Lloyd, et al, 1977, Malik, et al, 1994, Pieribatestti, et al, 1981, Stahl, et al, 1983, Vernin and Parkanyi, 2003) (Barbosa, et al, 2007, Bauer and Brasil, 1973, Lloyd, et al, 1977, Malik, et al, 1994, Pieribatestti, et al, 1981…”

“…It has been used in folk medicine as anti-inflammatory, antipyretic, analgesic and as a depurative agent. In addition, S. terebinthifolius has been used to treat sexually transmitted diseases, uterine inflammation, urinary tract infections, skin ulcers and gastroduodenal disorders (Cavalher-Machado, et al, 2008, Diniz, et al, 1997, Martinez, et al, 1996. Bacchi (1986) reports the use of aroeira species in the treatment of skin, mucous membrane injuries, ulcers and infections of the respiratory, digestive and genitourinary systems…”

Schinus terebinthifolius raddi (anacardiaceae) no processo de cicatrização de gastrorrafias em ratos

“…Furthermore, studies on the composition of plant parts, isolated compounds such as: essential oils, flavonoids, fractions enriched of flavonoids, Shinol (flavonoid) and masticadienoic acid, biflavonoids isolated and triterpenes. Have been studied the morphology and anatomy of the seed, barks and leaves, chemical and microscopic examination of the plant, barks development and fruits development, even clinical drug trials (phase I) of an herbal compound (Schinus, Plectranthus and Eucalyptus) 1,3,4,5,7,8,9,10,11,15,18,19,20,21,23,25,26,28,30,31,32,33,34 .…”

Mast cell concentration and skin wound contraction in rats treated with Brazilian pepper essential oil (Schinus terebinthifolius Raddi)

“…In popular medicine, it has been used as a treatment for respiratory infections and digestive disorders, such as gastritis, gastric atony and diarrhea. Extracts from BPT have antimicrobial 9,10 , antifungal 11 , antiparasitic 12 and antiinflammatory healing properties 13,14 and aren't toxic to humans 15 . Branco Neto et al 16 refers that the topic use of the hydroalcoholic extract of aroeira delayed the reepitelization of the skin wounds in rats.…”