Auxin-Regulated Transcription

Tj, Guilfoyle; Hagen, Gretchen; Li, Y; Ulmasov, Tim; Liu, ZB; Strabala, Timothy J.; Gee, Melissa A.

doi:10.1071/pp9930489

Cited by 66 publications

(53 citation statements)

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“…The GH3 gene identified in our study as upregulated by P. fluorescens in wild-type M. truncatula is part of a small multigene family in soybean (Glycine max; Hagen et al, 1991), and related genes exist in Arabidopsis (Guilfoyle et al, 1993). It belongs to early auxin-responsive genes that are rapidly activated at the transcriptional level by auxin-related compounds (Hagen and Guilfoyle, 1985;McClure et al, 1989;Koshiba et al, 1995).…”

Section: Root Colonization By P Fluorescens Triggers Enhanced Expresmentioning

confidence: 99%

Pseudomonas fluorescens and Glomus mosseae Trigger DMI3-Dependent Activation of Genes Related to a Signal Transduction Pathway in Roots of Medicago truncatula

et al. 2005

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Plant genes induced during early root colonization of Medicago truncatula Gaertn. J5 by a growth-promoting strain of Pseudomonas fluorescens (C7R12) have been identified by suppressive subtractive hybridization. Ten M. truncatula genes, coding proteins associated with a putative signal transduction pathway, showed an early and transient activation during initial interactions between M. truncatula and P. fluorescens, up to 8 d after root inoculation. Gene expression was not significantly enhanced, except for one gene, in P. fluorescens-inoculated roots of a Myc Nod2 genotype (TRV25) of M. truncatula mutated for the DMI3 (syn. MtSYM13) gene. This gene codes a Ca 21 and calmodulin-dependent protein kinase, indicating a possible role of calcium in the cellular interactions between M. truncatula and P. fluorescens. When expression of the 10 plant genes was compared in early stages of root colonization by mycorrhizal and rhizobial microsymbionts, Glomus mosseae activated all 10 genes, whereas Sinorhizobium meliloti only activated one and inhibited four others. None of the genes responded to inoculation by either microsymbiont in roots of the TRV25 mutant. The similar response of the M. truncatula genes to P. fluorescens and G. mosseae points to common molecular pathways in the perception of the microbial signals by plant roots.Root exudates play an active role in the regulation of symbiotic and pathogenic interactions with microbes (Hirsch et al., 2003). Rhizosphere microorganisms in turn can have a decisive influence on plant health. The most commonly studied beneficial interactions are the arbuscular mycorrhizal (AM) symbiosis, between the majority of land plant families and fungi in the phylum Glomeromycota (Smith and Read, 1997;Schü ssler et al., 2001), and the nodule symbiosis restricted to legumes and bacteria of the family Rhizobiaceae (Dénarié et al., 1992;Franssen et al., 1992;Perret et al., 2000). Several other genera of soil bacteria, including Pseudomonas and Bacillus species, can stimulate root proliferation or have antagonistic effects on pathogens in the rhizosphere (Kloepper and Schroth, 1978;Ellis et al., 2000;Whipps, 2001). Contrary to the AM and nodule symbioses, few investigations have focused on the molecular bases of plant responses to rhizobacteria such as Pseudomonas, and studies have mainly been concerned with elucidating the basis of induced systemic resistance by Pseudomonas species in Arabidopsis (Arabidopsis thaliana; Cartieaux et al., Verhagen et al., 2004;Wang et al., 2005).The lack of information about plant gene expression during root colonization by Pseudomonas fluorescens prompted us to exploit suppressive subtractive hybridization (SSH; Diatchenko et al., 1996) to identify plant gene responses during early-stage colonization of Medicago truncatula Gaertn. roots by P. fluorescens strain C7R12. P. fluorescens strain C7R12 is a spontaneous rifampicin-resistant mutant of a wild-type strain C7 (Eparvier et al., 1991) previously isolated from the rhizosphere of flax cultivated in a soil ...

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Section: Root Colonization By P Fluorescens Triggers Enhanced Expresmentioning

confidence: 99%

Pseudomonas fluorescens and Glomus mosseae Trigger DMI3-Dependent Activation of Genes Related to a Signal Transduction Pathway in Roots of Medicago truncatula

et al. 2005

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“…This class comprises related genes GHZ from soybean (Guilfoyle et al, 1993), PS-IAA4/5 and PS-IAAG from pea (Theologis et al, 1985), ARG3 and ARG4 from mung bean (Yamamoto et al, 1992), and at least 14 expressed genes from Arabidopsis, IAAZ to IAAZ4, which include AtAux2-2 1 (IAA4) and AtAux2-27 (IAA5) (Conner et al, 1990;Abel et al, 199513). In a specific response to auxin, steady-state mRNA levels of most Auxl IAA genes rapidly increase within 5 to 60 min.…”

Section: Prlmary Cenes Responsive To a U X I Nmentioning

confidence: 99%

Early Genes and Auxin Action

Abel

Theologis²

1996

Plant Physiology

695

427

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“…SAURs are abundant in the zone of elongation in soybean hypocotyls and are expressed most strongly in epidermal and cortical cells (6). Meanwhile SAURs have been identified in a variety of organisms, in Vigna radiata (7), Pisum sativum (8), Arabidopsis thaliana (9), Nicotiana tabacum (10), Raphanus sativus (11), Malus domestica (12) and more recently also in Zea mays (13). Genome analysis in A. thaliana reveals more than 70 SAUR homologs, which mostly are found in clusters (3).…”

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confidence: 99%

The Auxin-induced Maize Gene ZmSAUR2 Encodes a Short-lived Nuclear Protein Expressed in Elongating Tissues

Knauss

Rohrmeier

Lehle

2003

Journal of Biological Chemistry

112

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By differential screening of a cDNA library from auxin-induced maize coleoptiles we have isolated and characterized a SAUR gene, designated ZmSAUR2, belonging to a not yet characterized subtype of the SAUR family. ZmSAUR2 encodes a 15.3-kDa protein and is specifically induced by auxin in elongating coleoptile tissue but not in primary leaves or in roots. The transcript level rapidly increased within minutes and preceded auxin-stimulated elongation of coleoptile segments. Cycloheximide also induced ZmSAUR2 transcription, as has been shown for other early auxin-induced genes, whereas abscisic acid, brassinolide, ethylene, gibberellic acid, kinetin, and methyl jasmonate did not provoke an increase in ZmSAUR2 mRNA abundance. In pulse-chase experiments using auxin-induced coleoptiles and an anti-Zm-SAUR2 antibody we were able to precipitate a protein of the expected molecular mass and to determine a half-life of about 7 min, which is among the shortest known in eukaryotes. In gel shift assays binding of calmodulin to ZmSAUR2 was demonstrated, suggesting the possibility of post-transcriptional regulation. Upon transformation of onion epidermal cells with a ZmSAUR2::GUS construct the corresponding chimeric protein was detected in the nucleus. The results suggest that ZmSAUR2 encodes a short-lived nuclear protein that might be involved in auxin-mediated cell elongation.

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Auxin-Regulated Transcription

Cited by 66 publications

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Pseudomonas fluorescens and Glomus mosseae Trigger DMI3-Dependent Activation of Genes Related to a Signal Transduction Pathway in Roots of Medicago truncatula

Pseudomonas fluorescens and Glomus mosseae Trigger DMI3-Dependent Activation of Genes Related to a Signal Transduction Pathway in Roots of Medicago truncatula

Early Genes and Auxin Action

The Auxin-induced Maize Gene ZmSAUR2 Encodes a Short-lived Nuclear Protein Expressed in Elongating Tissues

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