Autoradiographic Study of Insulin Distribution in the Rat with Special Reference to Elastic Tissue

Worthington, W. C.; Jones, Douglas J.; Buse, Maria G.

doi:10.1210/endo-74-6-914

Cited by 9 publications

(5 citation statements)

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“…This was confirmed with the immunoperoxidase technique. Although these findings have not been reported for PTH, localization of insulin (33)(34)(35), glucagon (36), growth hormone (37)(38)(39), luteinizing hormone, and follicle-stimulating hormone (40,41), human chorionic gonadotropin (42), and prolactin (43) (13), and our current data-showing that localization of 125I-labeled and unlabeled preparations are identical, indicate that the metabolism ofthe radioiodinated PTH used in these studies accurately reflects the metabolism of unlabeled hormone.…”

Section: Discussioncontrasting

confidence: 53%

Analysis of Parathyroid Hormone and Its Fragments in Rat Tissues

D'Amour¹,

Segre²,

Roth³

et al. 1979

J. Clin. Invest.

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A B S T R A C T After intravenous injection of [125LI]iodo-parathyroid hormone in the rat, uptake of the hormone was greatest in the liver and kidneys. Uptake was rapid, reaching a maximal concentration by 4 and 8 min, respectively. Extracts, prepared from both these organs at intervals soon after the injection of intact hormone, showed three main radioactive peaks when samples were subjected to gel filtration under proteindenaturing conditions. The first peak coeluted with intact hormone.The second eluted at a position corresponding to the carboxy-terminal fragments previously described in plasma, and the last eluted at the salt volume of the column. Microsequence analysis of the radioiodinated fragments, a method that has proved valuable for chemically defining the circulating fragments resulting from metabolism of injected hormone, showed that extracts of liver and kidney, prepared at 4 and 8 min after injection of the intact hormone, contained different fragments. The radioiodinated fragments in liver extracts were identical to those previously reported in the plasma of rats and dogs, fragments resulting principally from proteolysis between positions 33 and 34, and 36 and 37 of the intact hormone. Although the same fragments were also present in the kidneys, they constituted less than 15% of the amount present in the liver. More than 50% of the

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Section: Discussioncontrasting

confidence: 53%

Analysis of Parathyroid Hormone and Its Fragments in Rat Tissues

D'Amour¹,

Segre²,

Roth³

et al. 1979

J. Clin. Invest.

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“…As in the :rat [cf Worthington et al (2)], 125 I induced silver grains were present at very high concentration in kidney cortex radioautog;rams, and at high concentration in radioautograms of liver and duodenal mucosa (lamina propria areas). Fig.…”

Section: Resultsmentioning

confidence: 94%

“…(2) reported that, in rat liver radioautograms, insulin-125 I induced grains were at 15 minute sacrifice time more highly concentrated near Kupffer than parenchymal cells. In the present experiments this relation was noted at all sacrifice times used; the higher concentration of grains over Kupffer than over parenchymal cells was especially noticeable for the \, 1 and 2 minute sacrifice mice (c/ Fig.…”

Section: *V ••>/-**-' • ' •• •'mentioning

confidence: 97%

“…Their radioautographic findings provide further support for this interpretation; in kidneys of rats sacrificed 15 minutes after insulin-131 I injection, radioactivity was most highly concentrated in proximal tubule areas. Worthington et al (2) substituted insulin-125 I to secure sharper radioautograms, and reported that there was a reversal in position of maximum radioactivity in the rat kidney from above proximal tubule lumina at 1-minute sacrifice to above proximal tubule cells at 15-minute sacrifice. We have endeavored to add to the above-described findings by using mice, by increasing the number of sacrifice times after insulin-125 I injection, and by estimating whether tissues which exhibited significant radioactivity as determined through radioautography also contained significant amounts of intact insulin.…”

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confidence: 99%

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Evidence from Combined Immunoassay and Radioautography Procedures That Intact Insulin-¹²⁵I Molecules Are Concentrated by Mouse Kidney Proximal Tubule Cells

et al. 1967

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Tissue samples were obtained from mice sacrificed 4 to 25 min after tail vein injection of m I-labeled beef insulin, 12 m^g and 0.1 Mc/g or 60 m/ug and 0.5 fic/g mouse. The extent to which 126 I present in tissues was attached to intact insulin-m I molecules was judged through measurement of the reactivity of 125 I in each tissue homogenate with cellulose and with guinea pig anti-insulin serum (AIS), which abolishes insulin reactivity with cellulose. Tissue radioautograms were also prepared. In the liver, the 126 I was preferentially concentrated by Kupffer cells and rapidly lost its reactivity to cellulose and, by inference, to AIS also. Kidney 125 I concentration continued to increase for at least 10 min; at 5 min and shorter sacrifice times, over 40 % of the 126 I was still reactive with both cellulose and AIS, i.e., still attached to intact insulin molecules. From the radioautograms, it was judged that by far the greater part of the 126 I was in the cortex. This 125 I was present at highest concentration in the glomeruli at \ min, in glomeruli and lumina of the necks between glomeruli and proximal tubules at 1 min, in proximal tubule lumina at 2 min, and in proximal tubule cells at 5, 10 and 25 min. These data indicate that the ability of mouse kidney to concentrate 125 I of labeled insulin is due to abilities of the glomeruli to filter labeled insulin molecules, and of proximal tubule cells to re-absorb these labeled insulin molecules. {Endocrinology 81: 475, 1967)

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“…2]3) and the reports of others are contradictory. Thus parenchymal cells [10], Kupffer cells [1], both [20] and neither [18] of these hepatic cell types have been reported to accumulate injected labeled insulin in normal animals. In the kidneys, as uniformly reported elsewhere [1, t0, 20], labeled in-derived from endogenous sources but it is clear from the results shown in Table 2 that unlabeled as well as labeled insulin concentrates transiently in both the livers and kidneys of normal rats.…”

Section: Discussionmentioning

confidence: 98%

Distribution of injected insulin and insulin-antibody complexes in normal and insulin-immunized animals

et al. 1974

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The distribution of insulin injeebed into normal rats and guinea pigs as the free hormone or as insulin-antibody complexes was studied by extraction of immunoreaetive insulin and by following the fate of 12aI-or ~31I-labeled insulin in the plasma, liver, kidneys and spleens of the injected animals. Injected free hormone rapidly disappears from the plasma to accumulate transiently in the liver, and, to a greater extent, in the proximal convoluted tubules of the kidneys. When insulin-antibody complexes formed in vitro are injected, the hormone disappears more slowly from the plasma and concentrates in the liver and spleen where it can be demonstrated by autoradiography in reticulo-endothelial cells; little or none is found in the kidneys. After injection into insulin-immunized guinea pigs, the hormone disappears very slowly from the plasma and accumulates almost exclusively in the liver with no evidence of concentration by any particular cell type; little or none is found in the kidneys or spleen.

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Autoradiographic Study of Insulin Distribution in the Rat with Special Reference to Elastic Tissue

Cited by 9 publications

References 0 publications

Analysis of Parathyroid Hormone and Its Fragments in Rat Tissues

Analysis of Parathyroid Hormone and Its Fragments in Rat Tissues

Evidence from Combined Immunoassay and Radioautography Procedures That Intact Insulin-¹²⁵I Molecules Are Concentrated by Mouse Kidney Proximal Tubule Cells

Distribution of injected insulin and insulin-antibody complexes in normal and insulin-immunized animals

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Autoradiographic Study of Insulin Distribution in the Rat with Special Reference to Elastic Tissue

Cited by 9 publications

References 0 publications

Analysis of Parathyroid Hormone and Its Fragments in Rat Tissues

Analysis of Parathyroid Hormone and Its Fragments in Rat Tissues

Evidence from Combined Immunoassay and Radioautography Procedures That Intact Insulin-125I Molecules Are Concentrated by Mouse Kidney Proximal Tubule Cells

Distribution of injected insulin and insulin-antibody complexes in normal and insulin-immunized animals

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Evidence from Combined Immunoassay and Radioautography Procedures That Intact Insulin-¹²⁵I Molecules Are Concentrated by Mouse Kidney Proximal Tubule Cells